宋志慧;王庆伟;

• 1:青岛科技大学环境与安全工程学院

摘要(Abstract)：

为了研究重金属对水生生物的联合毒性作用,以斑马鱼为受试生物,采用半静态法,在研究Cu2+、Cd2+和Cr6+对斑马鱼单一毒性的基础上,以获得的Cu2+、Cd2+和Cr6+的96h-LC50数据,利用等效应曲线法研究了Cu-Cd、Cu-Cr和Cd-Cr的联合毒性作用,并综合分析环境毒理学指标的测定结果,考察利用斑马鱼作为水质监测指示生物的可行性。结果表明:Cu2+、Cd2+和Cr6+对斑马鱼的96h-LC50分别为0.296、17.72和64.64mg·L-1,毒性顺序为Cu2+>Cd2+>Cr6+,其中Cu2+为高毒物质,Cd2+和Cr6+为低毒物质;Cu-Cd、Cu-Cr和Cd-Cr对斑马鱼毒性作用类型分别为协同作用、拮抗作用和协同作用。CAT活性与Cu2+、Cd2+和Cr6+浓度均具有显著的剂量-效应关系,因此斑马鱼可以作为监测重金属污染的指示生物。

关键词(KeyWords)： 斑马鱼;重金属;LC50;联合作用;生物预警

Abstract:

Keywords:

基金项目(Foundation): 山东省社会科学规划项目(03CJZ03);; 山东省高等学校实验技术研究项目(2005-188)

作者(Author): 宋志慧;王庆伟;

Email:

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