三氯酚暴露致稀有鮈鲫肝脏损伤的比较蛋白质组研究Proteomic Analysis of Hepatic Protein Profiles in Rare Minnow (Gobiocypris rarus)Exposed to Trichlorophenol(TCP)
房彦军;高先军;査金苗;宁保安;李晓丽;马新华;高志贤;晁福寰;
摘要(Abstract):
为探讨2,4,6-三氯酚(2,4,6-TCP)暴露致鱼类肝脏损伤的毒性效应,以稀有鮈鲫(Gobiocypris rarus)为模式生物,TCP暴露浓度为1、10、100μg·L-1,进行流水暴露实验(28d),利用二维凝胶电泳方法(2-DE),结合质谱分析技术(MALDI-TOF-MS),对TCP致稀有鮈鲫肝脏损伤的蛋白表达谱进行了研究.结果显示,共发现35个差异蛋白点,其中11个蛋白被成功鉴定(C.I.>95%).对鉴定的蛋白根据GOTerms进行功能分类,发现它们主要参与脂类代谢与转运、氧化应激以及蛋白修复和氧化磷酸化等生物学过程。以上结果表明,TCP对稀有鮈鲫具有明显的肝脏毒性效应.
关键词(KeyWords): 三氯酚;稀有鮈鲫;肝脏;蛋白质组
基金项目(Foundation): 国家自然科学青年基金项目(No.20707039)
作者(Author): 房彦军;高先军;査金苗;宁保安;李晓丽;马新华;高志贤;晁福寰;
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