房彦军;高先军;査金苗;宁保安;李晓丽;马新华;高志贤;晁福寰;

• 1:军事医学科学院卫生学环境医学研究所天津环境与食品安全风险监控技术重点实验室
• 2:中国科学院生态环境研究中心环境水质学国家重点实验室

摘要(Abstract)：

为探讨2,4,6-三氯酚(2,4,6-TCP)暴露致鱼类肝脏损伤的毒性效应,以稀有鮈鲫(Gobiocypris rarus)为模式生物,TCP暴露浓度为1、10、100μg·L-1,进行流水暴露实验(28d),利用二维凝胶电泳方法(2-DE),结合质谱分析技术(MALDI-TOF-MS),对TCP致稀有鮈鲫肝脏损伤的蛋白表达谱进行了研究.结果显示,共发现35个差异蛋白点,其中11个蛋白被成功鉴定(C.I.>95%).对鉴定的蛋白根据GOTerms进行功能分类,发现它们主要参与脂类代谢与转运、氧化应激以及蛋白修复和氧化磷酸化等生物学过程。以上结果表明,TCP对稀有鮈鲫具有明显的肝脏毒性效应.

关键词(KeyWords)： 三氯酚;稀有鮈鲫;肝脏;蛋白质组

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学青年基金项目(No.20707039)

作者(Author): 房彦军;高先军;査金苗;宁保安;李晓丽;马新华;高志贤;晁福寰;

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