塔娜;房彦军;林本成;张伟;田蕾;袭著革;

• 1:军事医学科学院卫生学环境医学研究所天津环境与食品安全风险监控技术重点实验室

摘要(Abstract)：

为研究新型阻燃剂磷酸三(2,3-二氯丙基)酯(TDCPP)的毒性效应,以稀有鮈鲫(Gobiocypris rarus)为实验生物,采用半静态实验方法,分别进行96 h和28 d的染毒,研究TDCPP对稀有鮈鲫的急性毒性和慢性毒性效应,并通过对稀有鮈鲫脑组织中与神经纤维的生长、发育、轴突再生等相关基因mRNA表达量的检测,初步探讨了TDCPP的神经毒性作用。结果表明:TDCPP对稀有鮈鲫的96 h-LC50为2.99(2.20~3.38)mg·L-1,根据化学物质对鱼类毒性分级标准TDCPP属于剧毒性。经0.9、1.5、2.1和2.7 mg·L-1TDCPP染毒28 d,与对照组相比,暴露组稀有鮈鲫肝脏及脑组织中SOD和GSH-Px的活性均受到抑制,且随着TDCPP的暴露浓度增加,其抑制作用显著增强;暴露组稀有鮈鲫脑组织中与神经纤维的生长、发育相关的微管蛋白α/β、神经丝NF-M以及关键蛋白GAP-43等基因的mRNA表达量,与对照相比均发生显著下调。可知,TDCPP暴露可以诱发稀有鮈鲫神经毒性作用。

关键词(KeyWords)： 磷酸三(2,3-二氯丙基)酯;稀有鮈鲫;毒性效应;基因表达

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学青年基金(20707039);; 天津市科技攻关课题(11ZCKFSF01100)

作者(Author): 塔娜;房彦军;林本成;张伟;田蕾;袭著革;

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