磷酸三(2,3-二氯丙基)酯阻燃剂对稀有鮈鲫的毒性效应Toxic Effect of Tri( 2,3-Dichloroprophyl) Phosphate Flame Retardant on Rare Minnow( Gobiocypris rarus)
塔娜;房彦军;林本成;张伟;田蕾;袭著革;
摘要(Abstract):
为研究新型阻燃剂磷酸三(2,3-二氯丙基)酯(TDCPP)的毒性效应,以稀有鮈鲫(Gobiocypris rarus)为实验生物,采用半静态实验方法,分别进行96 h和28 d的染毒,研究TDCPP对稀有鮈鲫的急性毒性和慢性毒性效应,并通过对稀有鮈鲫脑组织中与神经纤维的生长、发育、轴突再生等相关基因mRNA表达量的检测,初步探讨了TDCPP的神经毒性作用。结果表明:TDCPP对稀有鮈鲫的96 h-LC50为2.99(2.20~3.38)mg·L-1,根据化学物质对鱼类毒性分级标准TDCPP属于剧毒性。经0.9、1.5、2.1和2.7 mg·L-1TDCPP染毒28 d,与对照组相比,暴露组稀有鮈鲫肝脏及脑组织中SOD和GSH-Px的活性均受到抑制,且随着TDCPP的暴露浓度增加,其抑制作用显著增强;暴露组稀有鮈鲫脑组织中与神经纤维的生长、发育相关的微管蛋白α/β、神经丝NF-M以及关键蛋白GAP-43等基因的mRNA表达量,与对照相比均发生显著下调。可知,TDCPP暴露可以诱发稀有鮈鲫神经毒性作用。
关键词(KeyWords): 磷酸三(2,3-二氯丙基)酯;稀有鮈鲫;毒性效应;基因表达
基金项目(Foundation): 国家自然科学青年基金(20707039);; 天津市科技攻关课题(11ZCKFSF01100)
作者(Author): 塔娜;房彦军;林本成;张伟;田蕾;袭著革;
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