郑丽萍;王国庆;林玉锁;冯艳红;吴春宇;张亚;

• 1:环境保护部南京环境科学研究所
• 2:国家环境保护土壤环境管理与污染控制重点实验室

摘要(Abstract)：

选取我国贵州省某矿区典型的重金属复合污染土壤开展28 d慢性毒性暴露试验,以蚯蚓体内2种抗氧化酶活性——过氧化氢酶(CAT)和超氧化物歧化酶(SOD)、8-羟基脱氧鸟苷(8-ODHG)和金属硫蛋白(MT)含量为生物指标,评估了以上生物指标与土壤重金属含量的相关性,探讨该研究方法用于矿区土壤重金属生态风险评估的可行性。结果表明,CAT和SOD在暴露周期内均出现先诱导后抑制的动态响应过程,表明蚯蚓通过提高CAT和SOD的活性来清除机体因污染胁迫而产生的自由基,以适应环境变化,在毒性缓解后,蚯蚓体内代谢产物的积累抑制了抗氧化氢酶活性,CAT和SOD活性下降并趋于稳定。暴露于重金属污染亚致死浓度时,蚯蚓体内的8-OHDG含量随着暴露时间的延长显著降低,随着暴露浓度的增加,损伤存在由低到高的转变。在28 d暴露周期内,蚯蚓体内MT在重金属胁迫下发挥了解毒作用,MT含量在蚯蚓暴露后第7天增加,随后逐渐降低。本文基于生物毒性试验综合评估了矿区典型重金属污染土壤的生态风险,研究结果对指导重金属复合污染土壤的修复与再利用具有重要意义。

关键词(KeyWords)： 重金属;蚯蚓;抗氧化酶;8-羟基脱氧鸟苷;金属硫蛋白;生态风险评价

Abstract:

Keywords:

基金项目(Foundation): 贵州省重大科技计划课题(黔科合重大专项字[2012]6014号);; 2014年环保公益性行业科研专项项目(201409041和201409042)

作者(Author): 郑丽萍;王国庆;林玉锁;冯艳红;吴春宇;张亚;

Email:

DOI:

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