史雅静;徐晓宇;王宇;韩雪松;李田春;王玉荣;史雅娟;

• 1:辽宁科技学院生物医药与化学工程学院辽宁省生物医药与化学工程重点实验室
• 2:中国科学院生态环境研究中心城市与区域生态国家重点实验室

摘要(Abstract)：

以自然土壤为介质,考察赤子爱胜蚓(Eisenia fetida)的生长抑制率、蛋白质含量、超氧化物歧化酶(SOD)活性、谷胱甘肽转移酶(GST)活性等指标,进行了急性(14 d)、亚急性(28 d)暴露下四溴双酚A(tetrabromobisphenol A,TBBPA)非致死剂量胁迫对蚯蚓的生长和抗氧化防御效应研究。结果表明:在急性试验中TBBPA对蚯蚓的生长抑制均存在显著的剂量效应关系,而在亚急性暴露期,400 mg·kg~(-1)时显著抑制蚯蚓的生长(P<0.05);TBBPA对蚯蚓体内蛋白含量影响,在急性试验中各处理间差异不显著(P=0.712),进入亚急性期蛋白质含量在400 mg·kg~(-1)时被显著诱导(P=0.039,P<0.05);TBBPA对蚯蚓SOD酶活性的影响,在急性试验中50 mg·kg~(-1)时被显著诱导(P<0.01),在亚急性试验中400 mg·kg~(-1)时SOD酶活性显著升高(P<0.05);TBBPA对蚯蚓GST酶活性影响,在急性试验中400 mg·kg~(-1)时呈现显著诱导效应(P<0.05),在亚急性期各处理间差异不显著(P=0.428)。蚯蚓生长抑制率、蛋白质含量、体内各生化酶系对TBBPA暴露的时间效应和剂量效应的敏感性存在不同程度差异,应依据污染暴露指标的有效性和敏感性选择多时间段检测和多指标进行土壤安全评价诊断。

关键词(KeyWords)： 四溴双酚A;赤子爱胜蚓;生长抑制率;蛋白质含量;SOD;GST

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(No.41671483);; 国家国际科技合作专项(No.2012DFA91150)

作者(Author): 史雅静;徐晓宇;王宇;韩雪松;李田春;王玉荣;史雅娟;

Email:

DOI:

参考文献(References)：

• [1]魏建良.四溴双酚A(TBBPA)的研发生产现状与质量提升[J].盐业与化工,2014,43(4):4-7Wei J L.Research and development production status and quality improvement of tetrabromobisphenol A(TBBPA)[J].Journal of Salt and Chemical Industry,2014,43(4):4-7(in Chinese)
• [2]Iasur-Kruh L,Ronen Z,Arbeli Z,et al.Characterization of an enrichment culture debrominating tetrabromobisphenol A and optimization of its activity under anaerobic conditions[J].Journal of Applied Microbiology,2010,109(2):707-715
• [3]Celada L J,Whalen M M.The effect of TBBPA on MAP3Ks in human natural killer cells[J].The FASEB Journal,2013,27(1):1042-1045
• [4]Fujii Y,Nishimura E,Kato Y,et al.Dietary exposure to phenolic and methoxylated organohalogen contaminants in relation to their concentrations in breast milk and serum in Japan[J].Environment International,2014,63:19-25
• [5]Harrad S,Abdallah A E,Rose N L,et al.Current-use brominated flame retardants in water,sediment,and fish from English lakes[J].Environmental Science and Technology,2009,43(24):9077-9083
• [6]Johnson-Restrepo B,Adams D H,Kannan K.Tetrabromobisphenol A(TBBPA)and hexabromocyclododecanes(HBCDs)in tissues of humans,dolphins,and sharks from the United States[J].Chemosphere,2008,70(11):1935-1944
• [7]Lu Z,Letcher R J,Chu S,et al.Spatial distributions of polychlorinated biphenyls,polybrominated diphenyl ethers,tetrabromobisphenol A and bisphenol A in Lake Erie sediment[J].Journal of Great Lakes Research,2015,41(3):808-817
• [8]Ni H-G,Zeng H.HBCD and TBBPA in particulate phase of indoor air in Shenzhen,China[J].Science of the Total Environment,2013,458:15-19
• [9]钱艳园,刘莉莉,于晓娟,等.四溴双酚A好氧降解菌的筛选及其降解特性研究[J].环境科学,2012,33(11):3962-3966Qian Y Y,Liu L L,Yu X J,et al.Isolation of aerobic degrading strains for TBBPA and the properties of biodegradation[J].Environmental Science,2012,33(11):3962-3966(in Chinese)
• [10]Colnot T,Kacew S,Dekant W.Mammalian toxicology and human exposures to the flame retardant 2,2',6,6'-tetrabromo-4,4'-isopropylidenediphenol(TBBPA):Implications for risk assessment[J].Archives of Toxicology,2014,88(3):553-573
• [11]He M-J,Luo X-J,Yu L-H,et al.Diasteroisomer and enantiomer-specific profiles of hexabromocyclododecane and tetrabromobisphenol A in an aquatic environment in a highly industrialized area,South China:Vertical profile,phase partition,and bioaccumulation[J].Environmental Pollution,2013,179:105-110
• [12]陈海,孙云娜,魏东洋.溴代阻燃剂的环境危害[J].广东化工,2011,38(8):91-92Chen H,Sun Y N,Wei D Y.Bromination flame retardants environmental damage[J].Guangdong Chemical Industry,2011,38(8):91-92(in Chinese)
• [13]胡玲,林玉锁.呋喃丹对赤子爱胜蚓体内蛋白含量,SOD和TCh E活性的影响[J].安徽农业科学,2006,34(13):3165-3167Hu L,Lin Y S.Effect of carbofuran on protein content and the SOD and TCh E activity of the Eisenia foetida earthworm[J].Journal of Agricultural Science,2006,34(13):3165-3167(in Chinese)
• [14]刘凯,杨亚军,田俊策,等.种植Bt水稻后的土壤对赤子爱胜蚓生长发育及酶活性的影响[J].生物安全学报,2015,24(3):225-231Liu K,Yang Y J,Tian J C,et al.Effect of soil planted with Bt rice on the development and enzyme activities of Eisenia foetida[J].Journal of Biosafety,2015,24(3):225-231(in Chinese)
• [15]王飞菲,郑梦梦,刘树海,等.两种除草剂对蚯蚓的急性毒性及氧化胁迫效应[J].生态毒理学报,2014,9(6):1210-1218Wang F F,Zheng M M,Liu S H,et al.Acute toxicity and oxidative stress of two herbicides on earthworm Eisenia fetide[J].Asian Journal of Ecotoxicology,2014,9(6):1210-1218
• [16]Gerecke A C,Giger W,Hartmann P C,et al.Anaerobic degradation of brominated flame retardants in sewage sludge[J].Chemosphere,2006,64(2):311-317
• [17]Hu F,Pan L,Xiu M,et al.Bioaccumulation and detoxification responses in the scallop Chlamys farreri exposed to tetrabromobisphenol A(TBBPA)[J].Environmental Toxicology and Pharmacology,2015,39(3):997-1007
• [18]Ji C,Li F,Wang Q,et al.An integrated proteomic and metabolomic study on the gender-specific responses of mussels Mytilus galloprovincialis to tetrabromobisphenol A(TBBPA)[J].Chemosphere,2016,144:527-539
• [19]陈玛丽,瞿璟琰,刘青坡,等.四溴双酚-A和五溴酚对红鲫肝脏组织和超微结构的影响[J].安全与环境学报,2008,8(4):8-11Chen M L,Qu J Y,Liu Q P,et al.Effects of tetrabromobisphenol A and pentabromophenol on the liver histological and ultra-microstructure of Carassius auratus[J].Journal of Safety and Environment,2008,8(4):8-11(in Chinese)
• [20]苏燕,范涤敏,罗义,等.四溴双酚A对鲫鱼血清抗氧化系统的影响[J].南京大学学报:自然科学版,2007,43(2):164-170Su Y,Fan D M,Luo Y,et al.Antioxidant response in serum of Carassius auratus under tetrabromobisphenol A exposure[J].Journal of Nanjing University:Science Edition,2007,43(2):164-170(in Chinese)
• [21]张丽,蒋凤华,曹为,等.四溴双酚A(TBBPA)对中肋骨条藻的毒性效应研究[J].生态毒理学报,2014,9(6):1219-1225Zhang L,Jiang F H,Cao W,et al.Toxic effects of tetrabromobisphenol A(TBBPA)on Skeletonema costatum[J].Asian Journal of Ecotoxicology,2014,9(6):1219-1225(in Chinese)
• [22]Chen C,Xue S,Zhou Q,et al.Multilevel ecotoxicity assessment of polycyclic musk in the earthworm Eisenia fetida using traditional and molecular endpoints[J].Ecotoxicology,2011,20(8):1949-1958
• [23]范学铭,史小航,王哲娟,等.玉迪安对蚯蚓3种抗氧化酶活性的影响[J].中国农学通报,2010,26(13):319-323Fan X M,Shi X H,Wang Z J,et al.Effect of dean on Eisenis foetida three antioxidant enzymes activities[J].Chinese Agricultural Science Bulletin,2010,26(13):319-323(in Chinese)
• [24]郑丽萍,王国庆,林玉锁,等.贵州省典型矿区土壤重金属污染对蚯蚓的毒性效应评估[J].生态毒理学报,2015,10(2):258-265Zheng L P,Wang G Q,Lin Y S,et al.Evaluation of toxicity effects of heavy metal contaminated soils on earthworm(Eisenia foetida)in a mining area of Guizhou Province[J].Asian Journal of Ecotoxicology,2015,10(2):258-265(in Chinese)
• [25]Organization for Economic Co-operation and Development.Test 207:Earthworm,Acute Toxicity Tests[R].Paris:OECD,1984
• [26]郑晓奇,史雅娟,吕永龙,等.全氟辛烷磺酸对赤子爱胜蚓的抗氧化酶,代谢酶和DNA损伤的影响[J].环境科学学报,2013,33(11):3153-3159Zheng X Q,Shi Y J,LüY L,et al.Effects of perfluorooctane sulfonate on antioxidant and metabolic enzymes and DNA damage of earthworms(Eisenia fetida)[J].Acta Scientiae Circumstantiae,2013,33(11):3153-3159(in Chinese)
• [27]Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Analytical Biochemistry,1976,72(1-2):248-254
• [28]Habig W H,Pabst M J,Jakoby W B.Glutathione S-transferases the first enzymatic step in mercapturic acid formation[J].Journal of Biological Chemistry,1974,249(22):7130-7139
• [29]Saint-Denis M,Labrot F,Narbonne J,et al.Glutathione,glutathione-related enzymes,and catalase activities in the earthworm Eisenia fetida andrei[J].Archives of Environmental Contamination and Toxicology,1998,35(4):602-614
• [30]张薇,宋玉芳,孙铁珩,等.土壤低剂量荧蒽胁迫下蚯蚓的抗氧化防御反应[J].土壤学报,2007,44(6):1049-1057Zhang W,Song Y F,Sun T H,et al.Antioxiding defensive responses of earthworms(Eisenia fetida)exposed to lowdosage of fluoranthenein soil[J].Acta Pedologica Sinica,2007,44(6):1049-1057(in Chinese)
• [31]BielskáL,HovorkováI,KomprdováK,et al.Variability of standard artificial soils:Physico-chemical properties and phenanthrene desorption measured by means of supercritical fluid extraction[J].Environmental Pollution,2012,163(4):1-7
• [32]张敏,邢永雷,李成涛,等.PBS基共聚物对蚯蚓体内蛋白质含量的影响研究[J].环境科学与技术,2012,35(10):42-45Zhang M,Xing Y L,Li C T,et al.Effect of PBS-based copolymer on protein content of earthworm[J].Environmental Science and Technology,2012,35(10):42-45(in Chinese)
• [33]徐湘博,史雅娟,吕永龙,等.四溴双酚A对赤子爱胜蚓的生长和基因表达的影响[J].生态毒理学报,2014,9(6):1053-1059Xu X B,Shi Y J,Lv Y L,et al.Effects of tetrabromobisphenol A(TBBPA)on growth and gene expression of earthworms(Eisenia fetida)[J].Asian Journal of Ecotoxicology,2014,9(6):1053-1059(in Chinese)
• [34]晋艺聪,刘丽华,罗贤丽,等.四溴双酚-A的降解性和毒性研究进展[J].中央民族大学学报:自然科学版,2010,19(2):5-10Jin Y C,Liu L H,Luo X L,et al.Progress in study of degradability and ecotoxicology of tetrabromobisphenol A[J].Journal of MUC:Natural Sciences Edition,2010,19(2):5-10(in Chinese)
• [35]白桂芬,曾小波.敌百虫对蚯蚓体内3种抗氧化酶活性的影响[J].吉林农业科学,2013,38(1):22-26Bai G F,Zeng X B.Effect of trichlorfon on the activities of three antioxidant enzymes of earthworm[J].Journal of Jilin Agricultural Sciences,2013,38(1):22-26(in Chinese)
• [36]邰托娅,林玉锁,贺静.土壤中Cu和Pb单一及复合污染对蚯蚓体内蛋白含量和SOD活性的影响[J].农业环境科学学报,2008,27(5):1985-1990Tai T Y,Lin Y S,He J.Effect of Cu,Pb single and combined pollution on total protein content and SOD activity of the Eisenia foetida earthworm in soils[J].Journal of Agro-Environment Science,2008,27(5):1985-1990(in Chinese)
• [37]Gestel C A M V,Diepen A M F V.The influence of soil moisture content on the bioavailability and toxicity ofcadmium for Folsomia candida Willem(Collembola:Isotomidae)[J].Ecotoxicology and Environmental Safety,1997,36(2):123-132
• [38]Amorim M J D B,Sousa J P,Nogueira A J A,et al.Bioaccumulation and elimination of 14C-lindane by Enchytraeus albidus in artificial(OECD)and a natural soil[J].Chemosphere,2002,49(3):323-329
• [39]Hofman J,Rhodes A,Semple K T.Fate and behaviour of phenanthrene in the natural and artificial soils[J].Environmental Pollution,2008,152(2):468-475
• [40]Belfroid A C,Sijm D T H M,Gestel C A M V.Bioavailability and toxicokinetics of hydrophobic aromatic compounds in benthic and terrestrial invertebrates[J].Environmental Reviews,1996,4(4):276-299
• [41]Amorim M J,R9mbke J,Scheffczyk A,et al.Effect of different soil types on the enchytraeids Enchytraeus albidus and Enchytraeus luxuriosus using the herbicide phenmedipham[J].Chemosphere,2005,61(8):1102-1114
• [42]Sun K,Gao B,Zhang Z,et al.Sorption of atrazine and phenanthrene by organic matter fractions in soil and sediment[J].Environmental Pollution,2010,158(12):3520-3526
• [43]VlˇckováK,Hofman J.A comparison of POPs bioaccumulation in Eisenia fetida in natural and artificial soils and the effects of aging[J].Environmental Pollution,2012,160(1):49-56
• [44]BielskáL K,Hofman J.Supercritical fluid extraction of persistent organic pollutants from natural and artificial soils and comparison with bioaccumulation in earthworms[J].Environmental Pollution,2013,176(5):48-54
• [45]Amorim M J,R9mbke J,Schallnass H J,et al.Effect of soil properties and aging on the toxicity of copper for Enchytraeus albidus,Enchytraeus luxuriosu s,and Folsomia candida[J].Environmental Toxicology&Chemistry,2005,24(8):1875-1885
• [46]Spurgeon D J,Hopkin S P.Extrapolation of the laboratory-based OECD earthworm toxicity test to metal-contaminated field sites[J].Ecotoxicology,1995,4(3):190-205
• [47]Shi Y,Zhang Q,Huang D,et al.Survival,growth,detoxifying and antioxidative responses of earthworms(Eisenia fetida)exposed to soils with industrial DDT contamination[J].Pesticide Biochemistry and Physiology,2016,128:22-29