低浓度二噁英抑制青鳉幼鱼耳泡生成与SOX9b相关联The Relation Between SOX9b and TCDD Inhibited Development of Otic Vesicle in Medaka Embryos
于永利;王丰;陈形;于华荣;赵宝全;东彦新;杨景峰;董武;
摘要(Abstract)：
2,3,7,8-四氯二苯并对二恶英(2,3,7,8-Tetrachlorodibenzo-p-Dioxin,TCDD)是一种持久性环境有机污染物质,它在有机体内的蓄积性引起研究人员的重视。以青鳉胚胎作为实验动物模型,使用原位杂交和定量聚合酶链锁反应(Polymerase Chain Reaction,PCR)技术从分子水平探讨了TCDD对胚胎耳泡的发育及其毒性作用机制。结果表明,TCDD造成青鳉幼鱼耳泡生成障碍。通过原位杂交和q PCR分析表明,TCDD引起软骨发育的变化与SOX9b表达降低有密切的关联。研究认为青鳉胚胎是一种非常敏感的TCDD模式动物,其耳泡软骨发育以及SOX9b基因表达可能是一种二恶英发育毒性的效应标记物。
关键词(KeyWords)： TCDD;青鳉胚胎;耳泡;毒性
基金项目(Foundation): 内蒙古自治区自然科学基金(Nos.2012MS1203);; 国家自然科学基金项目(Nos.30360090;21267015);; 教育部新世纪优秀人才支持计划项目(Nos.NCET-04-0262);; 内蒙古自治区“草原英才”工程(2014)
作者(Author): 于永利;王丰;陈形;于华荣;赵宝全;东彦新;杨景峰;董武;
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