崔燕;白承连;徐涛;王晓彤;陈元红;靳大庆;

• 1:温州医学院水域科学与环境生态研究所 浙江省模式生物技术与应用重点实验室

摘要(Abstract)：

全氟辛酸(PFOA)是一种分布广泛的环境持久性有机污染物,在野生动物与人体内普遍被检出。目前PFOA发育毒性的特点和机制尚未阐明,已有的研究显示,PFOA毒性与受试物种及性别密切相关。因此,利用不同物种进行PFOA毒性研究对阐明其毒性机制十分重要。本研究选用了斑马鱼这种理想的脊椎动物模型,考察了PFOA暴露对其胚胎发育的自主运动、心跳、行为、细胞凋亡和DNA损伤的影响。研究发现,始于6hpf的PFOA暴露会导致斑马鱼胚胎自主运动异常,心率降低,且具有一定的剂量依赖性;6～24hpf的高浓度的PFOA暴露(>414.0mg·L-1)会导致胚胎发生显著的细胞凋亡,凋亡主要出现在眼部、头部、心脏和尾部。较高浓度的PFOA暴露(165.6mg·L-1)会使仔鱼的光刺激应激行为模式发生变化。此外,PFOA暴露会致使斑马鱼发生DNA损伤,且损伤程度随PFOA浓度的升高而加重,表明PFOA具有一定的基因毒性。上述结果表明,PFOA对斑马鱼胚胎具有发育毒性,具体表现为自主运动异常,心率降低和行为反应能力变弱,同时伴随畸形、细胞凋亡和DNA损伤。

关键词(KeyWords)： 全氟辛酸(PFOA);斑马鱼胚胎;发育毒性;行为反应;DNA损伤

Abstract:

Keywords:

基金项目(Foundation): 浙江省教育厅科研计划项目(Y201010056);; 温州市科技局国际合作项目(H20100062);; 浙江省自然科学基金一般项目(Y2110659);; 国家环保公益性行业科研专项(200909089)

作者(Author): 崔燕;白承连;徐涛;王晓彤;陈元红;靳大庆;

Email:

DOI:

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