邻苯二甲酸二乙基己酯(DEHP)对金鲫鱼脑细胞DNA的损伤DNA Damage in Brain Cells of Gold Crucian Carp Induced by Di-(2-ethylhexyl) Phthalate(DEHP)
陈莉;李学彬;杨光涛;邓灵福;丁书茂;
摘要(Abstract):
为了研究邻苯二甲酸二乙基己酯(DEHP)对金鲫鱼(Carassius auratu)脑细胞DNA的损伤作用,采用不同浓度的DEHP溶液(0、25、50、100、200mg·L-1)对体外培养脑细胞进行染毒,应用单细胞凝胶电泳(彗星实验)检测脑细胞DNA的损伤效应.结果表明,染毒1.5h后,与对照组相比,DEHP各染毒组细胞尾部DNA百分率(Tail DNA%)和尾矩(Tail Moment)均显著升高(p<0.01),即DEHP染毒引起了脑细胞DNA的严重断裂;随着DEHP浓度的增加,DNA损伤程度加剧,细胞尾部DNA百分率及尾距与DEHP染毒浓度呈明显的剂量-效应关系.以上结果表明,DEHP可导致金鲫鱼脑细胞DNA损伤。
关键词(KeyWords): DEHP;单细胞凝胶电泳;DNA损伤;金鲫鱼;脑细胞
基金项目(Foundation): 国家科技支撑计划项目(No.2006BAJ02A10)
作者(Author): 陈莉;李学彬;杨光涛;邓灵福;丁书茂;
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参考文献(References):
- Calafat A M,Needham L L,Silva M J,Lambert G.2004.Exposure to di-(2-ethylhexyl)phthalate among premature neonatesin a neonatal intensive care unit[J].Pediatrics,113:429-434
- Dzhekova-Stojkova S,Bogdanska J,Stojkova Z.2001.Peroxisomeproliferators:their biological and toxicological effects[J].Clinical Chemistry and Laboratory Medicine,39:468-474
- Doull J,Cattley R,Elcombe C,Lake B G,Swenberg J,Wilkinson C,Williams G,Van Gemert M.1999.A cancer riskassessment of di(2-ethylhexyl)phthalate:Application of the new U.S.EPA risk assessment guidelines[J].Regulatory Toxicologyand Pharmacology,29:327-357
- Fromme H,Kuchler T,Otto T,Pilz K,Miiller J,Wenzel A.2002.Occurrence of phthalates and bisphenol A and F in theenvironment[J].Water Research,36:1429-1438
- Gaunt I F,Butterworth K R.1982.Autoradiographic study oforally administered di-(2-ethylhexyl)phthalate in the mouse[J].Food Chemical Toxicology,20:215-217
- Harris C A,Henttu P,Parker M G,Sumpter J P.1997.Theestrogenic activity of phthalate esters in vitro[J].Environmental Health Perspectives,105:802-811
- Hu C L,Zhong L F.2007.Genotoxic effects of di(2-ethylhexyl)phthalate in humans and animals[J].Journal of Dalian Medical University,29:185-190(in Chinese)
- Kozumbo W J,Rubin R J.1991.Mutagenicity and metabolismof dimethyl phthalate and its binding to epidermal and hepaticmacromolecules[J].Toxicology and Environmental Health,33:29-46
- Lottrup G,Andersson A M,Leffers H,Mortensen G K,Toppari J,Skakkebaek N E,Main K M.2006.Possible impact ofphthalates on infant reproductive health[J].International Journalof Andrology,29:172-180,discussion181-185
- Lu Z S,Yan Y,Qiao Y,Yao H C,Yang X.2003.Studies ongenotoxicity of gaseous formaldehyde on human buccal cells[J].China Environmental Science,23:566-569(in Chinese)
- Ma J H,LüL X,Li S G.2005.Study on the gene expressedprofile of di(2-ethylhexyl)phthalate-induced brain tissue in Drosophila melanogaster[J].Journal of Environmental and Occupational Medicine,22:91-94(in Chinese)
- Mao C X,Tian X K,Yang G T,Yang C,Qiao Y K,Li Y,Jing L D,Yang X.2007.DNA damage of Hela cells inducedby nanosized Mn O2and normal Mn O2powder[J].Asian Journalof Ecotoxicology,2:190-194(in Chinese)
- Marsee K,Woodruff T J,Axelrad D A,Calafat A M,Swan SH.2006.Estimated daily phthalate exposures in a population ofmothers of male infants exhibiting reduced anogenital distance[J].Environmental Health Perspectives,114:805-809
- Mc Kee R H,Butala J H,David R M,Gans G.2004.NTPcenter for the evaluation of risks to human reproduction reportson phthalates:addressing the data gaps[J].Reproductive Toxicology,18:1-22
- Mihovec-Grdic'M,S#mit Z,Puntaric'D,Bos#nir J.2002.Phthalatesin underground waters of the Zagreb area[J].Croatian Medical Journal,43:493-497
- Park J D,Habeebu S S M,Klaassen C D.2002.Testiculartoxicity of di-(2-ethylhexyl)phthalate in young Sprague-Dawleyrats[J].Toxicology,171:105-115
- Sha Y,Xia X,Yang Z,Huang G H.2006.Distribution of PAEs in the middle and lower reaches of the Yellow River,China[J].Environmental Monitoring and Assessment,124:277-287
- Tice R R,Donner E M,Andrews P W,Vasquez M.1997.The SCG/Comet assay:Applications to environmental and humanbiomonitoring[J].Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis,379:S127
- 胡存丽,仲来福.2007.邻苯二甲酸(2-乙基己基)酯遗传毒性研究进展[J].大连医科大学学报,29:185-190
- 鲁志松,严彦,乔琰,姚汉超,杨旭.2003.气态甲醛对人体颊黏膜细胞遗传毒性的研究[J].中国环境科学,23:566-569
- 马建红,吕立夏,厉曙光.2005.邻苯二甲酸二酯诱导果蝇脑组织基因差异表达谱的研究[J].环境与职业医学,22:91-94
- 毛彩霞,田熙科,杨光涛,杨超,乔永康,李岩,景连东,杨旭.2007.纳米Mn O2与常规Mn O2粉末对Hela细胞DNA损伤的对比研究[J].生态毒理学报,2:190-194
- 王蕊,李厚勇,王子兰,郭启明,刘永霞,陶玉珍,高晓奇.2002.邻苯二甲酸(2-乙基己基)酯致畸致突变实验研究[J].癌变·畸变·突变,14:120-121
- 张蕴晖.2002.邻苯二甲酸二乙基己酯对环境和生物体的危害[J].国外医学:卫生学分册,29:73-77