邻苯二甲酸二乙基己酯(DEHP)对金鲫鱼脑细胞DNA的损伤DNA Damage in Brain Cells of Gold Crucian Carp Induced by Di-(2-ethylhexyl) Phthalate(DEHP)
陈莉;李学彬;杨光涛;邓灵福;丁书茂;
摘要(Abstract)：
为了研究邻苯二甲酸二乙基己酯(DEHP)对金鲫鱼(Carassius auratu)脑细胞DNA的损伤作用,采用不同浓度的DEHP溶液(0、25、50、100、200mg·L-1)对体外培养脑细胞进行染毒,应用单细胞凝胶电泳(彗星实验)检测脑细胞DNA的损伤效应.结果表明,染毒1.5h后,与对照组相比,DEHP各染毒组细胞尾部DNA百分率(Tail DNA%)和尾矩(Tail Moment)均显著升高(p<0.01),即DEHP染毒引起了脑细胞DNA的严重断裂;随着DEHP浓度的增加,DNA损伤程度加剧,细胞尾部DNA百分率及尾距与DEHP染毒浓度呈明显的剂量-效应关系.以上结果表明,DEHP可导致金鲫鱼脑细胞DNA损伤。
关键词(KeyWords)： DEHP;单细胞凝胶电泳;DNA损伤;金鲫鱼;脑细胞
基金项目(Foundation): 国家科技支撑计划项目(No.2006BAJ02A10)
作者(Author): 陈莉;李学彬;杨光涛;邓灵福;丁书茂;
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