牛海岗;王宏元;张育辉;

• 1:陕西师范大学生命科学学院

摘要(Abstract)：

为评价双酚A(BPA)对中国林蛙(Rana chensinensis)蝌蚪的急性毒性,将26期的蝌蚪暴露于浓度为2.4×10-5mol·L-1～4.2×10-5mol·L-1BPA的水体中进行急性毒性实验.结果表明,24、48、72、96h蝌蚪的死亡几率(y)与浓度对数(x)的回归方程分别为y=16.915x-4.1157、y=22.11x-6.1905、y=20.766x-5.3871、y=20.715x-5.351;半数致死浓度(LC50)分别为3.47×10-5、3.28×10-5、3.20×10-5、3.16×10-5mol·L-1.安全浓度(SC)为0.88×10-5mol·L-1.为探讨在安全浓度以下BPA对中国林蛙蝌蚪生长发育的影响,将林蛙蝌蚪分别于10-5、10-6、10-7mol·L-1BPA的水体中连续暴露直至完全变态,并设10-8、10-9mol·L-1雌二醇(E2)的阳性对照和空白对照,分别测定并统计蝌蚪发育所需的发育时间、体长和体质量.结果表明,蝌蚪对10-5、10-6、10-7mol·L-1BPA与10-8、10-9mol·L-1E2的效应相似,可延缓林蛙幼体的发育时间,导致体长和体质量降低.推测低浓度BPA抑制蝌蚪生长发育的机制之一是干扰了正常的内分泌活动.

关键词(KeyWords)： 双酚A;中国林蛙;蝌蚪;生长发育;毒性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(No.30770243)

作者(Author): 牛海岗;王宏元;张育辉;

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参考文献(References)：

• Alford R A,Dixon P M,Pechmann J H K.2001.Ecology:Global amphibian population declines[J].Nature,412:499-500
• Bgi C,Schwaiger J,Ferling H,Mallow U,Steineck C,Sinowatz F,Kalbfus W,Negele R D,Lutz I,Kloas W.2003.Endocrine effects of environmental pollution on Xenopus laevisand Rana temporaria[J].Environmental Research,93(2):195-201
• Duan Z H,Zhu L,Zhu L Y,Yao K,Zhu X S.2008.Individualand joint toxic effects of pentachlorophenol and bisphenol A onthe development of zebrafish(Danio rerio)embryo[J].Ecotoxicologyand Environmental Safety,71(3):774-780
• Gosner K L.1960.A simplified table for staging anuran embryosand larvae with notes of identification[J].Herpetologica,16:183-190
• Hayashi H,Nishimoto A,Oshima N,Iwamuro S.2007.Expression of the estrogen receptor alpha gene in the anal fin ofJapanese medaka,Oryzias latipes,by environmental concentrationsof bisphenol A[J].Journal of Toxicological Sciences,32(1):91-96
• Hayes T B,Case P,Chui S,Chung D,Haeffele C,Haston K,Lee M,Mai V P,Marjuoa Y,Parker J,Tsui M.2006.Pesticidemixtures,endocrine disruption,and amphibian declines:are weunderestimating the impact[J].Environmental Health Perspectives,114(Supppl.1):40-50
• Imai S,Koyama J,Fujii K.2005.Effects of17β-estradiol onthe reproduction of Java-medaka(Oryzias javanicus),a new testfish species[J].Marine Pollution Bulletin,51(8-12):708-714
• Iwamuro S,Sakakibara M,Terao M,Ozawa A,Kurobe C,Shigeura T,Kato M,Kikuyama S.2003.Teratogenic and anti-metamorphic effects of bisphenol A on embryonic and larvalXenopus laevis[J].General and Comparative Endocrinology,133(2):189-198
• Kloas W,Lutz I,Einspanier R.1999.Amphibians as a model tostudy endocrine disrupters:II.Estrogenic activity of environmentalchemicals in vitro and in vivo[J].Science of the TotalEnvironment,225(1-2):59-68
• Krishnan A V,Stathis P,Permuth S F,Tokes L,Feldman D.1993.Bisphonel-A:an estrogenic substance is released frompolycarbonate flasks during autoclaving[J].Endocrinology,132:2279-2286
• Levy G,Lutz I,Krüger A,Kloas W.2004.Bisphenol A inducesfeminization in Xenopus laevis tadpoles[J].Environmental Research,94(1):102-111
• Liu H L,Liu X H,Wang X Y,Wang X R,Yu H X.2007.Toxicity of BPA and TBBPA to Daphnia magna and ZebrafishBrachydanio rerio[J].Environmental Science,28(8):1784-1787(in Chinese)
• Lv X F,Zhou Q F,Song M Y,Jiang G B,Shao J.2007.Vitellogenic responses of17β-estradiol and bisphenol A in maleChinese loach(Misgurnus anguillicaudatus)[J].EnvironmentalToxicology and Pharmacology,24(2):155-159
• Marquis O,Millery A,Guittonneau S,Miaud C.2006.Solventtoxicity to amphibian embryos and larvae[J].Chemosphere,63(5):889-892
• Pickford D B,Hetheridge M J,Caunter J E,Hall A T,Hutchinson T H.2003.Assessing chronic toxicity of bisphenol Ato larvae of the African clawed frog(Xenopus laevis)in a flow-through exposure system[J].Chemosphere,53(3):223-235
• Ramakrishnan S,Wayne N L.2008.Impact of bisphenol-A onearly embryonic development and reproductive maturation[J].Reproductive Toxicology,25(2):177-183
• Shi G,Wang J X,Wang R X.2007.Toxic effects of Cr6+ongrowth and development of tadpole of Rana chensinensis[J].Acta Agriculturae Zhejiangensis,19(3):220-224(in Chinese)
• Sone K,Hinago M,Kitayama A,Morokuma J,Ueno N,Watanabe H,Iguchi T.2004.Effects of17beta-estradiol,nonylphenol,and bisphenol-A on developing Xenopus laevisembryos[J].General and Comparative Endocrinology,138(3):228-236
• Wen R S,Fang Z Q,Chen W T.2008.Effects of17β-estradiolon vitellogenin inducement and gonad development in maleTanichthys albonubes[J].Zoological Research,29(1):43-48(inChinese)
• Wistuba J,Brinkworth M H,Schlatt S,Chahoud I,Nieschlag E.2003.Intrauterine bisphenol A exposure leads to stimulatoryeffects on Sertoli cell number in rats[J].EnvironmentalResearch,91(2):95-103
• Zhuang H S,Yang G.2005.Study on the acute and subacutetoxicities of Bisphenol A on the carp[J].EnvironmentalChemistry,24(6):682-684(in Chinese)
• 刘红玲,刘晓华,王晓祎,王晓蓉,于红霞.2007.四溴双酚A对大型溞和斑马鱼的毒性［J].环境科学,28(8):1784-1787
• 石戈,王健鑫,王日昕.2007.Cr6+对中国林蛙蝌蚪生长发育的毒性效应[J].浙江农业学报,19(3):220-224
• 温茹淑,方展强,陈伟庭.2008.17β-雌二醇对雄性唐鱼卵黄蛋白原的诱导及性腺发育的影响[J].动物学研究,29(1):43-48
• 庄惠生,杨光.2005.双酚A对鲤鱼急性和亚急性毒性的研究[J].环境化学,24(6):682-684