王贺威;马胜伟;张喆;陈海刚;黄志斐;巩秀玉;蔡文贵;贾晓平;

• 1:中国水产科学研究院南海水产研究所 广东省渔业生态环境重点实验室 农业部南海渔业资源环境重点野外科学观测实验站
• 2:上海海洋大学海洋科学学院
• 3:上海海洋大学水产与生命学院

摘要(Abstract)：

为了解全氟辛烷磺酸盐(PFOS)对海洋贝类抗氧化防御系统的毒性效应及致毒机理,在实验室条件下研究了PFOS对翡翠贻贝的96h急性毒性,同时探讨PFOS胁迫和净水恢复过程中翡翠贻贝外套膜和内脏团组织中抗氧化指标(SOD活性、GSH和MDA含量)的变化。结果显示,PFOS对翡翠贻贝的96h半致死浓度(LC50)为68.3mg·L-1,安全浓度为6.83mg·L-1。在PFOS胁迫阶段,1mg·L-1浓度组外套膜SOD活性显著性升高(p<0.05),内脏团SOD酶活性显著降低(p<0.05);而PFOS浓度高于1mg·L-1时,外套膜SOD活性显著性降低(p<0.05),内脏团SOD活性显著升高(p<0.05)。PFOS胁迫对翡翠贻贝外套膜和内脏团中GSH含量均有显著的诱导作用(p<0.05),PFOS胁迫15d后各浓度组GSH含量均受到显著的抑制(p<0.05)。翡翠贻贝外套膜MDA含量受PFOS胁迫后显著升高(p<0.05),内脏团MDA含量的变化呈先降低、后升高的规律。净水释放阶段,翡翠贻贝两组织中SOD活性在释放7d后均恢复至对照组水平,GSH含量和MDA含量呈显著升高的趋势(p<0.05)。研究结果表明,PFOS暴露能够引起翡翠贻贝外套膜和内脏团氧化胁迫,但这种损伤的效果不明显,释放短时间后即可自我恢复。

关键词(KeyWords)： 翡翠贻贝;全氟辛烷磺酸钾;超氧化物歧化酶(SOD);谷胱甘肽(GSH);丙二醛(MDA)

Abstract:

Keywords:

基金项目(Foundation): 广东省科技计划项目(2009B030600001);广东省科技计划项目(2011A060902006);; 广东省渔业生态环境重点实验室重点开放课题(GDKL2010-001);; 中央级公益性科研院所基本科研业务费专项资金项目(2009YD01、2010YD04、2010TS12)

作者(Author): 王贺威;马胜伟;张喆;陈海刚;黄志斐;巩秀玉;蔡文贵;贾晓平;

Email:

DOI:

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