胡伟;李冬梅;韩晓冬;刘红玲;于红霞;

• 1:南京大学环境学院污染控制与资源化研究国家重点实验室
• 2:南京大学医学院南京大学医学院免疫与生殖生物学实验室

摘要(Abstract)：

以原代培养的大鼠睾丸支持细胞为研究对象,选取环境及生物体中均有检出的一种典型的羟基化PBDE——6-HO-BDE-137作为目标化合物,设置0、0.1、1、10μmol·L-14个浓度梯度,应用噻唑蓝(MTT)试验、荧光显微镜观察和AnnexinV-FITC/PI双染流式细胞术,通过检测大鼠睾丸支持细胞增殖活性、细胞形态和细胞凋亡坏死率的变化,探讨其对支持细胞的损伤情况.研究发现,6-HO-BDE-137显著影响支持细胞的增殖活力,暴露24h,10μmol·L-1浓度组细胞与对照组相比显著增殖(p<0.05),随暴露时间的延长(至48h),10μmol·L-1组转为增殖抑制;支持细胞形态也表现出不同程度的改变,10μmol·L-1浓度组变化最为明显,有大量的细胞变圆飘起;随6-HO-BDE-137暴露浓度的升高,支持细胞死亡率增加,凋亡是支持细胞死亡的主要方式.研究结果提示:6-HO-BDE-137具有生殖毒性.

关键词(KeyWords)： 多溴联苯醚;6-HO-BDE-137;大鼠;睾丸支持细胞

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金委员会与香港研究资助局联合基金项目(No.20518002;No.N_CityU110/05);; 国家自然科学基金委员会重点基金项目(No.20737001)

作者(Author): 胡伟;李冬梅;韩晓冬;刘红玲;于红霞;

Email:

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