量子点环境暴露与细胞毒性效应研究进展Research Progress of Quantum Dots in Environmental Exposure and Cytotoxic Effects
柏杉山;张静姝;李青;刘克明;郭昌胜;王玉秋;
摘要(Abstract)：
量子点作为一种新型的荧光标记物近年来已在生物学中得到广泛应用.同时,量子点材料的大规模生产和应用对人体健康与生态环境可能产生的不利影响也引起了人们的普遍关注.论文概括了量子点的暴露途径,综述了机体对量子点的吸收、量子点在生物体内的转移、积累和清除的作用方式,重点阐述了不同类型量子点细胞毒性等方面的研究进展,最后,在目前研究的基础上对量子点生物安全性研究的发展方向进行了展望.
关键词(KeyWords)： 量子点;细胞毒性;Cd2+;氧化损伤
基金项目(Foundation): 教育部博士点基金项目(No.200800550011);; 天津市自然科学基金面上项目(No.2005KYZ45)
作者(Author): 柏杉山;张静姝;李青;刘克明;郭昌胜;王玉秋;
Email:
参考文献(References)：
- Akerman M E,Chan W C,Laakkonen P,Bhatia S N,RuoslahtiE.2002.Nanocrystal targeting in vivo[J].Proceedings of theNational Academy of Sciences,99:12617-12621
- Alivisatos A P.1996.Semiconductor clusters,nanocrystals,andquantum dots[J].Science,271:933-937
- Alivisatos A P.2004.The use of nanocrystals in biologicaldetection[J].Nature Biotechnology,22:47-52
- Ballou B,Lagerholm B C,Ernst L A,Bruchez M P,WaggonerA S.2004.Noninvasive imaging of quantum dots in mice[J].Bioconjugate Chemistry,15:79-86
- Breunig M,Bauer S,Goepferich A.2008.Polymers andnanoparticles:Intelligent tools for intracellular targeting?[J].European Journal of Pharmaceutics and Biopharmaceutics,68:112-128
- Chan W H,Shiao N H.2008.Cytotoxic effect of CdSe quantumdots on mouse embryonic development[J].Acta PharmacologicaSinica,29:259-266
- Chang E,Thekkek N,Yu W W,Colvin V L,Drezek R.2006.Evaluation of quantum dot cytotoxicity based on intracellularuptake[J].Small,2:1412-1417
- Cho S J,Maysinger D,Jain M,Rder B,Hackbarth S,WinnikF M.2007.Long-term exposure to CdTe quantum dots causesfunctional impairments in live cells[J].Langmuir,23:1974-1980
- Choi A O,Cho S J,Desbarats J,Lovric'J,Maysinger D.2007a.Quantum dot-induced cell death involves Fas upregulationand lipid peroxidation in human neuroblastoma cells[J].Journalof Nanobiotechnology,5:1
- Choi H S,Liu W,Misra P,Tanaka E,Zimmer Z P,Itty IpeB,Bawendi M G,Frangioni J V.2007b.Renal clearance ofquantum dots[J].Nature Biotechnology,25:1165-70
- Chu M Q,Wu Q,Wang J X,Hou S K,Miao Y,Peng J L,Sun Y.2007.In vitro and in vivo transdermal delivery capacityof quantum dots through mouse skin[J].Nanotechnology,18:5103-5108
- Derfus A M,Chan W C W,Bhatia S N.2004.Probing thecytotoxicity of semiconductor quantum dots[J].Nano Letter,4:11-18
- Fischer H C,Liu L,Pang K S,Chan W C W.2006.Pharmacokinetics of nanoscale quantum dots:in vivo distribution,sequestration,and clearance in the rat[J].Advanced FunctionalMaterials,16:1299-1305
- GagnéF,Auclair J,Turcotte P,Fournier M,Gagnon C,SauvéS,Blaise C.2008.Ecotoxicity of CdTe quantum dots tofreshwater mussels:Impacts on immune system,oxidative stressand genotoxicity[J].Aquatic Toxicology,86:333-340
- Gao X H,Cui Y Y,Levenson R M,Chung L W K,Nie S M.2004.In vivo cancer targeting and imaging with semiconductorquantum dots[J].Nature Biotechnology,22:969-976
- Green M,Howman E.2005.Semiconductor quantum dots andfree radical induced DNA nicking[J].Chemical Communications,7:121-123
- Hardman R.2006.A toxicologic review of quantum dots:toxicitydepends on physicochemical and environmental factors[J].Environmental Health Perspectives,114:165-172
- Hoshino A,Fujioka K,Oku T,Suga M,Sasaki Y F,Ohta T,Yasuhara M,Suzuki K,Yamamoto K.2004a.Physicochemicalproperties and cellular toxicity of nanocrystal quantum dotsdepend on their surface modification[J].Nano Letter,4:2163-2169
- Hoshino A,Hanaki K,Suzuki K,Yamamoto K.2004b.Applications of T-lymphoma labeled with fluorescent quantumdots to cell tracing markers in mouse body[J].Biochemical andBiophysical Research Communications,314:46-53
- Ipe B I,Lehnig M,Niemeyer C M.2005.On the generation offree radical species from quantum dots[J].Small,1:706-709
- Kim S,Lim Y T,Soltesz E G,De Grand A M,Lee J,Nakayama A,Parker J A,Mihaljevic T,Laurence R G,Dor DM,Cohn L H,Bawendi M G,Frangioni J V.2004.Near-infrared fluorescent type II quantum dots for sentinel lymph nodemapping[J].Nature Biotechnology,22:93-97
- Kirchner C,Liedl T,Kudera S,Pellegrino T,Muoz Javier AM,Gaub H E,Stlzle S,Fertig N,Parak W J.2005.Cytotoxicity of colloidal CdSe and CdSe/ZnS nanoparticles[J].Nano Letter,5:331-338
- Leroueil P R,Hong S,Mecke A,Baker J R Jr,Orr B G,Banaszak Holl M M.2007.Nanoparticle interaction withbiological membranes:does nanotechnology present a Janus face?[J].Accounts of Chemical Research,40:335-342
- Limbach L K,Wick P,Manser P,Grass R N,Bruinink A,Stark W J.2007.Exposure of engineered nanoparticles to humanlung epithelial cells:influence of chemical composition andcatalytic activity on oxidative stress[J].Environment Science&Technology,41:4158-416
- Lin C A J,Liedl T,Sperling R A,Fernández-Argüelles M T,Costa-Fernández J M,Pereiro R,Sanz-Medel A,Chang W H,Parak W J.2007.Bioanalytics and biolabeling with semiconductornanoparticles(quantum dots)[J].Journal of Materials Chemistry,17:1343-1346
- Loumbourdis N S,Danscher G.2008.Autometallographic tracingof Hg-S quantum dots in frogs exposed to inorganic mercury[J].Biometals,21:311-319
- Lovric'J,Bazzi H S,Cuie Y,Fortin G R A.Winnik F M,Maysinger D.2005a.Differences in subcellular distribution andtoxicity of green and red emitting CdTe quantum dots[J].Journal of Molecular Medicine,83:377-385
- Lovric'J,Cho S J,Winnik F M,Maysinger D.2005b.Unmodified cadmium telluride quantum dots induce reactiveoxygen species formation leading to multiple organelle damageand cell death[J].Chemistry&Biology,12:1227-1234
- Maysinger D.2007.Nanoparticles and cells:good companionsand doomed partnerships[J].Organic&Biomolecular Chemistry,5:2335-2342
- Medintz I L,Uyeda H T,Goldman E R,Mattoussi H.2005Quantum dot bioconjugates for imaging,labelling and sensing[J].Nature Materials,4:435-446
- Michalet X,Pinaud F F,Bentolila L A,Tsay J M,Doose S,LiJ J,Sundaresan G,Wu A M,Gambhir S S,Weiss S.2005.Quantum dots for live cells,in vivo imaging,and diagnostics[J].Science,307:538-544
- Nel A,Xia T,Mdler L,Li N.2006.Toxic potential ofmaterials at the nanolevel[J].Science,311:622-627
- Nemmar A,Hoet P H M,Vanquickenborne B,Dinsdale D,Thomeer M,Hoylaerts M F,Vanbilloen H,Mortelmans L,Nemery B.2002.Passage of inhaled particles into the bloodcirculation in humans[J].Circulation,105:411-414
- Oberdrster E.2004.Manufactured nanomaterials(fullerenes,C60)induce oxidative stress in the brain of juvenile largemouth bass[J].Environmental Health Perspectives,112:1058-1062
- Oberdrster G,Oberdrster E,Oberdrster J.2005.Nanotoxicology:an emerging discipline evolving from studies of ultrafine particles[J].Environmental Health Perspectives,113:823-839
- Oberdrster G,Sharp Z,Atudorei V,Elder A,Gelein R,Kreyling W,Cox C.2004.Translocation of inhaled ultrafineparticles to the brain[J].Inhalation Toxicology,16:437-445
- Robe A,Pic E,Lassalle H P,Bezdetnaya L,Guillemin F,Marchal F.2008.Quantum dots in axillary lymph node mapping:biodistribution study in healthy mice[J].BMC Cancer,8:111
- Ryman-Rasmussen J P,Riviere J E,Monteiro-Riviere N A.2006.Penetration of intact skin by quantum dots with diversephysicochemical properties[J].Toxicological Sciences,91:159-165
- Samia A C,Chen X,Burda C.2003.Semiconductor quantumdots for photodynamic therapy[J].Journal of the AmericanChemical Society,125:15736-15737
- Schipper M L,Cheng Z,Lee S W,Bentolila L A,Iyer G,RaoJ H,Chen X Y,Wu A M,Weiss S,Gambhir S S.2007.MicroPET-based biodistribution of quantum dots in living mice[J].Journal of Nuclear Medicine,48:1511-1518
- Shiohara A,Hoshino A,Hanaki K,Suzuki K,Yamamoto K.2004.On the cyto-toxicity caused by quantum dots[J].Microbiology and Immunology,48:669-675
- Tsay J M,Michalet X.2005.New light on quantum dotcytotoxicity[J].Chemistry&Biology,12:1159-1161
- Xi Z G,Lin Z Q.2006.Researching advancements andperspectives of the influences of nanoscale substances onecological environment and biological safety[J].Asian Journal ofEcotoxicology,1:203-208(in Chinese)
- Yang R S,Chang L W,Wu J P,Tsai M H,Wang H J,KuoY H,Yeh T K,Yang C S,Lin P.2007.Persistent tissuekinetics and redistribution of nanoparticles,quantum dot705,inmice:ICP-MS quantitative assessment[J].Environmental HealthPerspectives,115:1339-1343
- Yin F R,Gao Y X,Chen C Y,Wu G.2008.Toxicologicalstudy of quantum dot[J].Journal of Hygiene Research,37:760-763(in Chinese)
- 袭著革,林治卿.2006.纳米尺度物质对生态环境的影响及其生物安全性的研究进展与展望[J].生态毒理学报,1:203-208
- 尹芳蕊,高愈希,陈春英,吴刚.2008.量子点的毒理学研究进展[J].卫生研究,37:760-763