李世凯;江敏;吴昊;彭晓叶;张健龙;谢芹;刘利平;

• 1:上海海洋大学水产与生命学院
• 2:贵州省水产研究所

摘要(Abstract)：

为探讨抗寄生虫药物伊维菌素(IVM)对鱼类的毒性效应,以雄性吉富罗非鱼(Oreochromis niloticus)为实验对象,设定了A(空白对照)、B(乙醇对照)、C(0.1 mg·kg~(-1))、D(0.5 mg·kg~(-1))、E(1 mg·kg~(-1))5个实验组,研究IVM对吉富罗非鱼肝脏和血液生理生化的影响。研究发现:正常生理状态下吉富罗非鱼肝脏中的丙二醛(MDA)含量较低,IVM作用后除最高剂量E组中MDA含量在4 h、16 h和24 h时极显著高于对照组外,其余各组均未受到显著影响。较低注射剂量C组中超氧化物歧化酶(SOD)活性均得到了诱导,且均显著高于对照组,较高注射剂量组中除E组在第4 h、16 h和24 h SOD活性显著高于对照组外,其余均未发现有显著变化。肝脏中谷草转氨酶(AST)和谷丙转氨酶(ALT)在IVM作用下均发生了一定的变化,尤其是E组均显著低于对照组。与肝脏中的相反,血液中的AST则随剂量的升高而呈现增加的趋势,在某些时间点与对照组相比显著升高,而血液中ALT除C、D组个别时间点外其余的均未有显著变化。B、C、D、E 4组肝脏中的碱性磷酸酶(ALP)相对A组均发生了显著下降,但C、D、E组与B组除个别时间点外均未有显著差异,因此肝脏中的ALP变化可能是无水乙醇作用的结果,而非IVM。血液中ALP则均未有显著变化。研究表明高剂量的IVM对吉富罗非鱼的肝脏造成了一定影响,因此在实际使用过程中应选择合适的给药方式以及合理的给药剂量。

关键词(KeyWords)： 伊维菌素;吉富罗非鱼;酶活性;丙二醛

Abstract:

Keywords:

基金项目(Foundation): 欧盟科研架构计划(EU-FP7-SEAT222889);; 上海市教委重点学科建设项目(J50701);; 上海市高校知识服务平台项目(ZF1206)

作者(Author): 李世凯;江敏;吴昊;彭晓叶;张健龙;谢芹;刘利平;

Email:

DOI:

参考文献(References)：

• [1]Prichard R,Ménez C,Lespine A.Moxidectin and the avermectins:Consanguinity but not identity[J].International Journal for Parasitology Drugs and Drug Resistance,2012,2(12):134-153
• [2]江敏,彭章晓,吴昊,等.伊维菌素在水产养殖中的应用及其水生态风险[J].渔业现代化,2008,35(4):47-50Jiang M,Peng Z X,Wu H,et al.Application of ivermectin in aquaculture and corresponding aqua-ecosystem risk[J].Fishery Modernization,2008,35(4):47-50(in Chinese)
• [3]Campbel W C,Benz G W.Ivermectin:A review of efficacy and safety[J].Journal of Veterinary Pharmacology and Therapeutics,1984,7(1):1-16
• [4]Sanderson H,Laird B,Pope L,et al.Assessment of the environmental fate and effects of ivermectin in aquatic mesocosms[J].Aquatic Toxicology,2007,85(4):229-240
• [5]Palmer R,Rodger H,Drinan E,et al.Preliminary trials on the efficacy of ivermectin against parasitic copepods of Atlantic salmon[J].Bul etin of the European Association of Fish Pathologists,1987,7(2):47-54
• [6]Davies I M,Rodger G K.A review of the use of ivermectin as a treatment for sea lice[Lepeophtheirus salmonis(Kroyer)and Caligus elongatus Nordmann]infestation in farmed Atlantic salmon(Salmo salar L.)[J].Aquaculture Research,2000,31(11):869-883
• [7]Johnson S C,Margolis L.Efficacy of ivermectin for control of the salmon louse Lepeophtheirus salmonis on Atlantic salmon[J].Diseases of aquatic organisms,1993,17(2):101-105
• [8]吴昊,江敏,彭章晓,等.伊维菌素在环境中的降解及其对七种水生生物的急性毒性研究[J].水生生物学报,2012,36(5):965-970Wu H,Jiang M,Peng Z X,et al.Research on the degradation of ivermectin and its acute toxicity to seven aquatic organisms[J].Acta Hydrobiologica Sinica,2012,36(5):965-970(in Chinese)
• [9]Katharios P,Iliopoulou-Georgudaki J,Kapata-Zoumbos K,et al.Toxicity of intraperitoneally injected ivermectin in sea bream,Sparus aurata[J].Fish Physiology and Biochemistry,2001,25(2):99-108
• [10]Varo I,Rigos G,Navarro J C,et al.Effect of ivermectin on the liver of gilthead sea bream(Sparus aurata):A proteomic approach[J].Chemosphere,2010,80(5):570-577
• [11]Hy T,Horsberg T E,Nafstad I.The disposition of ivermectin in Atlantic salmon(Salmo salar)[J].Pharmacology&Toxicology,1990,67(4):307-312
• [12]Katharios P,Pavlidis M,Iliopoulou-Georgudaki J.Accumulation of ivermectin in the brain of sea bream,Sparus aurata after intraperitoneal administration[J].Environmental Toxicology and Pharmacology,2004,17(1):9-12
• [13]Toovey J P G,Lyndon A R,Duffus J H.Ivermectin inhibits respiration in isolated rainbow trout(Oncorhynchus mykiss Walbaum)gil tissue[J].Bul etin-European Association of Fish Pathologists,1999,19(4):149-152
• [14]Ucán-Marín F,Ernst W,O'Dor R K,et al.Effects of food borne ivermectin on juvenile Atlantic salmon(Salmo salar L.):Survival,growth,behavior,and physiology[J].Aquaculture,2012,334:169-175
• [15]韩冰,王荻,卢彤岩.伊维菌素对松浦镜鲤的急性毒性与组织损伤[J].中国水产科学,2015,22(4):687-697Han B,Wang D,Lu T Y.Acute toxicity and tissue injury studies of ivermectin for Songpu mirrorcarp(Cyprinus specularis Songpu)[J].Journal of Fishery Sciences of China,2015,22(4):687-697(in Chinese)
• [16]王荻,李绍戊,耿龙武,等.伊维菌素对雄性鲫的生殖毒性评价[J].应用生态学报,2015,26(10):3174-3180Wang D,Li S W,Geng L W,et al.Evaluation of ivermectins reproductive toxicity to male Carassius auratus[J].Chinese Journal of Applied Ecology,2015,26(10):3174-3180(in Chinese)
• [17]李世凯,张健龙,江敏,等.伊维菌素对斑马鱼(Danio rerio)生理生化特性的影响[J].安全与环境学报,2014,14(1):300-305Li S K,Zhang J L,Jiang M,et al.Effects of ivermectin on the physiological and biochemical characteristic features of Danio rerio[J].Journal of Safety and Environment,2014,14(1):300-305(in Chinese)
• [18]王文龙,崔欣,李成成,等.饲料中铜暴露对吉富罗非鱼幼鱼血红细胞微核和组织中铜蓄积的影响[J].生态毒理学报,2014,9(4):757-764(in Chinese)Wang W L,Cui X,Li C C,et al.Effects of dietary copper exposure on micronucleus of erythrocyte and accumulation in different tissues of juvenile GIFT tilapia(Oreochromis niloticus)[J].Asian Journal of Ecotoxicology,2014,9(4):757-764
• [19]农业部渔业渔政管理局.《2015中国渔业统计年鉴》[M].北京:中国农业出版社,2015:23-44
• [20]Rico A,Phu T M,Satapornvanit K,et al.Use of veterinary medicijnes,feed additives and probiotics in four major internationally traded aquaculture species farmed in Asia[J].Aquaculture,2013,412-1413(6):231-243
• [21]Shaikh B,Rummel N,Gieseker C,et al.Residue depletion of tritium-labeled ivermectin in the muscle tissues of aquacultured Atlantic salmon,tilapia,and catfish following oral treatment[J].North American Journal of Aquaculture,2012,74(1):27-33
• [22]Kanak E G,Dogan Z,Eroglu A,et al.Effects of fish size on the response of antioxidant systems of Oreochromis niloticus following metal exposures[J].Fish Physiology and Biochemistry,2014,40(4):1083-1091
• [23]于庆云,王悠,徐彦,等.镉和铅对菲律宾蛤仔脂质过氧化及抗氧化酶活性的影响[J].生态毒理学报,2013,8(4):504-512Yu Q Y,Wang Y,Xu Y,et al.Effects of cadmium and lead on the lipid peroxidation and levels of antioxidant enzymes in Ruditapes philippinarum[J].Asian Journal of Ecotoxicology,2013,8(4):504-512(in Chinese)
• [24]Pathan T S,Shinde S E,Thete P B,et al.Histopathology of liver and kidney of Rasbora daniconius exposed to paper mil effluent[J].Research Journal of Biological Sciences,2010,5(5):389-394
• [25]龙奕,刘珊珊,王萌,等.纳米Al2O3和Cd联合暴露对铜锈环棱螺体内Cd的生物积累和抗氧化酶活性的影响[J].生态毒理学报,2015,10(2):216-223Long Y,Liu S S,Wang M,et al.Effects of Cd and Al2O3-NPs co-exposure on bioaccumulation of Cd and antioxidase enzyme activities in Bel amya aeruginosa[J].Asian Journal of Ecotoxicology,2015,10(2):216-223(in Chinese)
• [26]Qureshi S.Biochemical toxicity of ivermectin in wistar albino rats[J].American-Eurasian Journal of Toxicological Sciences,2013,5(1):15-19
• [27]陈家长,杨光,马晓燕,等.低浓度阿维菌素对鲤鱼超氧化物歧化酶(SOD)的影响[J].生态毒理学报,2009,4(6):823-828Chen J C,Yang G,Ma X Y,et al.Effect of low concentration of avermectins on superoxide dismutase(SOD)ac-tivities in common carp[J].Asian Journal of Ecotoxicology,2009,4(6):823-828(in Chinese)
• [28]Gül爦,Belge-Kuruta爧E,Yildiz E,et al.Pollution correlated modifications of liver antioxidant systems and histopathology of fish(Cyprinidae)living in Seyhan Dam Lake,Turkey[J].Environment International,2004,30(5):605-609
• [29]Kavitha C,Malarvizhi A,Senthil Kumaran S,et al.Toxicological effects of arsenate exposure on hematological,biochemical and liver transaminases activity in an Indian major carp(Catla catla)[J].Food and Chemical Toxicology,2010,48(10):2848-2854
• [30]Nemcsok J,Benedeczky I.Effect of sublethal concentrations of phenol on some enzyme activities and blood sugar level of carp(Cyprinus carpio L.)[J].Environmental Monitoring and Assessment,1990,14(2-3):377-383
• [31]吴斌.小鼠伊维菌素急性与蓄积性毒性试验研究[D].成都:四川农业大学,2010:1-48Wu B.A Study on acute toxicity and accumulative toxicity of ivermectin in mice[D].Chengdu:Sichuan Agricultural University,2010:1-48(in Chinese)
• [32]彭章晓,江敏,吴昊,等.伊维菌素对鲫血液成分影响的初步研究[J].淡水渔业,2012,42(1):93-96Peng Z X,Jiang M,Wu H,et al.Preliminary study on the influence of ivermectin on blood component of Carassius auratus[J].Freshwater Fisheries,2012,42(1):93-96(in Chinese)
• [33]祁明亮,朱敏,赵晓祥.DEP、DOP暴露对鲤鱼的酸碱性磷酸酶的影响[J].安全与环境学报,2012,12(2):10-13Qi M L,Zhu M,Zhao X X.Effects of DEP and DOP exposure on the acid phosphatase and alkaline phosphatase in the crap[J].Journal of Safety and Environment,2012,12(2):10-13(in Chinese)