HgCl_2对斜生栅藻(Scenedesmus obliquus)生理生化特性的影响Effects of HgCl2 on Physiological and Biochemical Characteristics of Scenedesmus obliquus
牟文;熊丽;胡芹芹;周巧巧;杨雪;张灿;刘德立;
摘要(Abstract):
为探讨汞对藻类的毒性效应,以斜生栅藻(Scenedesmus obliquus)为实验材料,检测了HgCl2对藻细胞数目、光合色素含量、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量等生化指标的影响.结果表明,各暴露组(0.2~1.5mg·L-1)HgCl2对斜生栅藻的生长均有抑制作用,随HgCl2暴露浓度的增大,藻细胞密度逐渐降低,HgCl2对斜生栅藻的48、72、96hEC50值分别为1.194、1.113、0.986mg·L-1.随着HgCl2暴露浓度的升高(0.01~1.5mg·L-1),叶绿素a、b及类胡萝卜素等光合色素含量均呈逐渐降低趋势;SOD活性表现为先激活后抑制;MDA含量则显著增加(p<0.05,p<0.01).
关键词(KeyWords): 斜生栅藻;HgCl2;超氧化物歧化酶;丙二醛;光合色素
基金项目(Foundation): 国家自然科学基金(No.30771429;No.20672041)
作者(Author): 牟文;熊丽;胡芹芹;周巧巧;杨雪;张灿;刘德立;
Email:
DOI:
参考文献(References):
- Alam M G,Snow E T,Tanaka A.2003.Arsenic and heavy metalcontamination of vegetables grown in Samta village,Bangladesh[J].Science of the Total Environment,308(1-3):83-96
- Alberte R S,Friedman A L,Gustafson D L,Rudnick M S.1981.Light-harvesting systems of brown algae and diatomsisolation and characterization of chlorophyll a/c and chlorophyll afucoxanthin pigment-protein complexes[J].Biochimica etBiophysica Acta,635(2):304-316
- Diao S L.2007.The research of senate compares poison in thealgae acute toxicity monitor[J].Environmental Science andManagement,32(9):139-141(in Chinese)
- Du M.1996.Effect of mercury pollution on plasma membranepermeability of Silenastrum capricornutum and Anacystis nidulans[J].Journal of the Graduates Sun Yat-Sen University(NaturalSciences),17(2):46-49(in Chinese)
- Feng X B,Tang S L,Li Z G,Wang S F,Lian L.2004.Landfill site is an important source of atmospheric mercury[J].Chinese Science Bulletin,49(23):67-71(in Chinese)
- Geoffroy L,Dewez D,Vernet G,Popovic R.2003.differentphysiological parameters used in evaluation of oxyfluorfen effecton S.obliquus:validity of parameters as biomarkers[J].Archivesof Environmental Contamination and Toxicology,45(4):439-454
- Giannopolitis C N,Ries S K.1977.Superoxide dismutases II.Purification and quantitative relationship with water-soluble proteinin seedlings[J].Plant Physiology,59:315-318
- Gurbuz F,Ciftci H,Akcil A.2009.Biodegradation of cyanidecontaining effluents by Scenedesmus obliquus[J].Journal ofHazardous Materials,162(1):74-79
- Hu Q Q,Xiong L,Tianpei X Z,Li W Y.2008.Toxic effectsof dibutyl phthalate(DBP)on Scenedesmus obliquus[J].AsianJournal of Ecotoxicology,3(1):87-92(in Chinese)
- Hua Y F.1985.A study on the behavior of mercury inweathering environment in Wanshan district[J].Geochemica,19(1):1-5(in Chinese)
- Huang G L,Dai S G,Sun H W,Zhu N K,Wang H J,Xia XJ,Liu J L,Lang P Z,Lu G H.1994.Determination of thetoxicity of organic pollutants to algae[J].Environmental Chemistry,13(3):259-262(in Chinese)
- Kjellstrom T,Kennedy P,Wallis S,Mantell C.1986.Physicaland Mental Development of Children with Prenatal Exposure toMercury from Fish.Stage I:Preliminary Tests at Age4[R].Report3080.Solna,Sweden:National Swedish EnvironmentalProtection Board
- Kong F X,Hu W,Li J J,Liu Y.1998.Effects of low pH,Aland ratio of Ca to Al on the glutathione in green algaScenedesmus obliquus[J].Environmental Chemistry,17(5):422-427(in Chinese)
- Li H S,Sun Q,Zhao S J.2003.Manual Book of BotanicalPhysiology and Biochemistry[M].Beijing:Higher EducationPress(in Chinese)
- Li S H,Zhu H,Xia Y Z.1959.Single cell green algae batchculture[J].Acta Hydrobiologia Sinica,(4):463-472(in Chinese)
- Lin Y H,Zhang B Y,Lu J M,Liu L,Guan H H,Liu Z J,Guo S W,Zhao S M.1994.Methylation of mercury andmethylmercury metabolism in fish body[J].Journal of Fisheriesof China,18(4):326-329(in Chinese)
- Liu B Y,Zhou P J,Li J J,Wu Z L,Song L R.2006.Effectsofγ-HCH on the growth and photosystem and lipid peroxidationof Scenedesmus obliqnus kütz[J].Journal of Agro-EnvironmentalScience,25(1):204-207(in Chinese)
- Lowry O H,Rosebrough N J,Farr A L,Randall R J.1951.Protein measurement with the Folin phenol reagent[J].Journalof Biological Chemistry,193(1):265-275
- LüX Q,Sun B Y,Shi Z L.1990.Toxicity of mercury toPhaeodactylum tricornutum[J].Journal of Ocean University ofQingdao,20(4):85-92
- Mendola P,Selevan S G,Gutter S,Rice D.2002.Environmental factors associated with a spectrum ofneurodevelopmental deficits[J].Mental Retardation andDevelopmental Disabilities Research Reviews,8(3):188-197
- Su Q K,Jiang J Y,Ma Z D.2004.Status of mercurycontainment of Wuhan urban lake[J].Geological Science andTechnology Information,23(3):83-87(in Chinese)
- UNEP.2002.Report of the Global Mercury Assessment WorkingGroup on the Work of Its First Meeting[R].Geneva:UNEP
- Wen W R,Li P,Li H Y,Sun Y Z.2009.Research andthinking of the control of mercury pollution in China[J].Environmental Protection,(18):33-35(in Chinese)
- Xiong L,Wu Z B,Kuang Q J,Xia Y,He F.2002.Studies onthe toxicity of cypermethrin to Scenedesmus obliquus[J].ActaHydrobiologica Sinica,26(1):66-73(in Chinese)
- Xiong L,Xie P,Sheng X M,Wu Z B,Xie L Q.2005.Toxicity of cypermethrin on growth,pigments and superoxidedismutase of Scenedesmus obliquus[J].Ecotoxicology andEnvironmental Safety,60(2):188-192
- Yuan Z L,Ma X M,Han J F.2005.Effect of Cdcontamination on ultramicroscopic structure and some elementscontent of tobacco leaves[J].Acta Ecologica Sinica,25(11):2919-2927(in Chinese)
- Zhang Q Y,Li F D,Gao K C,Liu M Y,Ou G Q.2005.Effect of water stress on the photosynthetic capabilities and yieldof winter wheat[J].Acta Botanica Boreali-Occidentalia Sinica,25(6):1184-1190(in Chinese)
- Zhang Z.1999.The toxicity of heavy metals to the aquaticorganisms[J].River Pollution and Protection,10(2):28-33(inChinese)
- 刁树林.2007.藻类急性毒性检测中参比毒物的研究[J].环境科学与管理,32(9):139-141
- 杜敏.1996.汞污染对两种藻类质膜透性的影响[J].中山大学研究生学刊(自然科学版),17(2):46-49
- 冯新斌,汤顺林,李仲根,王少锋,Lian L.2004.生活垃圾填埋场是大气汞的重要来源[J].科学通报,49(23):67-71
- 胡芹芹,熊丽,田裴秀子,李文英.2008.邻苯二甲酸二丁酯(DBP)对斜生栅藻的致毒效应研究[J].生态毒理学报,3(1):87-92
- 花永丰.1985.万山地区风化环境中汞的行为探讨[J].地球化学,19(1):1-5
- 黄国兰,戴树桂,孙红文,竺廼恺,王怀瑾,夏晞娟,刘静玲,郎佩珍,陆光华.1994.有机污染物对藻类毒性的测定[J].环境化学,13(3):259-262
- 孔繁翔,胡伟,李继军,刘营.1998.低pH值,铝及钙铝比对斜生栅藻(Scenedesmus obliquus)谷胱甘肽含量影响研究[J].环境化学,17(5):422-427
- 黎尚豪,朱蕙,夏宜峥.1959.单细胞绿藻的大量培养试验[J].水生生物学集刊,(4):463-472
- 李合生,孙群,赵世杰.2003.植物生理生化试验原理和技术[M].北京:高等教育出版社
- 蔺玉华,张冰艳,卢健民,刘兰,关海红,刘子靖,郭少伟,赵淑敏.1994.鱼体汞的甲基化及其甲基汞的吸收与代谢[J].水产学报,18(4):326-329
- 刘碧云,周培疆,李佳洁,吴兆录,宋立荣.2006.丙体六六六对斜生栅藻生长及光合色素和膜脂过氧化影响的研究[J].农业环境科学学报,25(1):204-207
- 吕小乔,孙秉一,史致丽.1990.汞对三角褐指藻的毒性[J].青岛海洋大学学报,20(4):85-92
- 苏秋克,蒋敬业,马振东.2004.武汉市湖泊汞污染现状研究[J].地质科技情报,23(3):83-87
- 温武瑞,李培,李海英,孙阳昭.2009.我国汞污染防治的研究与思考[J].环境保护,(18):33-35
- 熊丽,吴振斌,况琪军,夏宜,贺锋.2002.氯氰菊酯对斜生栅藻的毒性研究[J].水生生物学报,26(1):66-73
- 袁祖丽,马新明,韩锦峰.2005.镉污染对烟草叶片超微结构及部分元素含量的影响[J].生态学报,25(11):2919-2927
- 张秋英,李发东,高克昌,刘孟雨,欧国强.2005.水分胁迫对冬小麦光合特性及产量的影响[J].西北植物学报,25(6):1184-1190
- 张征.1999.重金属对水生生物的毒性作用[J].水系污染与保护,10(2):28-33