梁艺怀;张琨;张京佶;刘敏;赵华清;殷浩文;

• 1:上海市检测中心生物与安全检测实验室

摘要(Abstract)：

为了验证我国本土常见的稀脉浮萍(Lemna aequinoctialis)是否适用于以青萍(Lemna minor)作为标准试验生物的生长抑制试验,对两种浮萍的毒性效应终点和毒性响应差异进行比较。通过对两种浮萍的总叶面积、干重、鲜重同叶状体数之间的相关性进行分析,根据《OECD化学品测试准则No.221浮萍生长抑制试验》,遵从良好实验室规范(GLP),将两种浮萍暴露于参比物质3,5-二氯苯酚,进行7 d更新式毒性试验,暴露浓度为(1.0~10)mg·L-1。结果表明,青萍的培养条件、试验方法以及毒性效应终点均适用于稀脉浮萍,两种浮萍的毒性试验呈现出相似的时间-效应关系和剂量-效应关系,两者的毒性效应浓度具有可比性,且数值上接近。以上实验结果可以得出,稀脉浮萍适用于生长抑制试验;鉴于稀脉浮萍在我国分布广泛、容易获取,稀脉浮萍可作为毒性测试的国家标准试验生物。

关键词(KeyWords)： 3,5-二氯苯酚;稀脉浮萍;青萍;生长抑郁;叶状体数;总叶面积

Abstract:

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基金项目(Foundation): 上海市科学技术委员会技术标准专项(12DZ0502600)

作者(Author): 梁艺怀;张琨;张京佶;刘敏;赵华清;殷浩文;

Email:

DOI:

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