姜会民;

• 1:山东高校十二五规划重点实验室"动物生理与生化及应用"重点实验室

摘要(Abstract)：

在急性毒性试验的基础上,设定0.052 mg·L-1(SL)、0.13 mg·L-1(SM)、0.26 mg·L-1(SH)三个汞离子染毒浓度和一个对照组(SC)研究无机汞污染对黄河鲤幼鱼鳃组织过氧化物酶(POD)活性和(丙二醛)MDA含量以及鳃组织损伤的影响。实验结果显示:POD活性随氯化汞浓度的增加先上升后下降,当浓度为0.26 mg·L-1时POD活性显著的低于对照组(p<0.05);实验组MDA含量均显著高于对照组(p<0.05);实验组鳃组织可见鳃小片肿胀,基部增生,上皮细胞脱落,泌氯细胞增多,细胞核溶解及金属沉积。试验结果表明氯化汞对鲤鳃组织结构和抗氧化系统具有明显的损伤。

关键词(KeyWords)： 氯化汞;鳃;过氧化物酶;丙二醛;组织损伤

Abstract:

Keywords:

基金项目(Foundation): 山东高校十二五规划重点实验室"动物生理与生化及应用";; 山东省教育厅基金项目(llLC53)

作者(Author): 姜会民;

Email:

参考文献(References)：

• [1]Liu Y Y,Shu H,Liu W.Study on enrichment of Songhua River methyl mercury in fish bodies and its affecting factors[J].Design of Hydroelectric Power Station,2004,20:63-66
• [2]Tomiyasu T,Matsuyama A,Eguchi T,et al.Spatial variations of mercury in sediment of Minamata Bay Japan[J].The Science of the Total Environment,6,368(1):283-290
• [3]Foestner U,Wittmann G T W.Metal Pollution in the Aquatic Environment[M].Springer Verlag,1979:267-268
• [4]Schroeder H A著.微量元素与人[M].北京:科学出版社,1979
• [5]Canli M,Robert W.Mercury and cadmium uptake from seawater and from food by the Norway lobster Nephrop norvegicus[J].Environmental Toxicology and Chemistry,1995,14(5):819-828
• [6]赵艳民,王新华,孙慧.Hg2+在中华绒螯蟹幼蟹鳃内的积累及其对组织结构的影响[J].动物学杂志,2008,43(1):1-7Zhao Y M,Wang X H,Sun H.Accumulation of waterborne mercury in gills of juvenile Eriocheir sinensis and its effect on gills structure[J].Chinese Journal of Zoology,2008,43(1):1-7(in Chinese)
• [7]蔺玉华,关海虹.甲基汞对鲤鱼鳃组织及氯细胞的影响[J].水产学杂志,2003,16(1):53-56Lan Y H,Guan H H.Effect of methylmercurity chloride on gills filaments and chloride cells of common carp[J].Chinese Journal of Fisheries,2003,16(1):53-56(in Chinese)
• [8]张毓琪,陈叙龙,杜式华,等.汞对鲤鱼(Cyprinus carpio L.)组织中某些元素含量比的影响[J].环境科学学报,1986(03):286-291Zhang Y Q,Chen X L,Du S H,et al.Effect of mercury accumulation on the content ratios of several elements in Carp(Cyprinus carpio L.)tissues[J].Acta Scientiae Circumstantiae,1986,(3):286-291(in Chinese)
• [9]彭德姣,侯娟,陈琳.无机汞在鲤鱼体内生物富集规律的研究[J].毒理学杂志,2007,21(4):129-135Peng D J,Hou J,Chen L.A study of the bioconcentration of inorganic mercury in Cyprinus carpio[J].Journal of Health Toxicology,2007,21(4):129-135(in Chinese)
• [10]修瑞琴,许永香,高世荣,等.砷与镉、锌离子对斑马鱼的联合毒性实验[J].中国环境科学,1998,18(4):349-352Xiu R Q,Xu Y,Gao S R,et al.Joint toxicity test of arsenic with cadmium and zinc ions to zebrafish,brachynanio rerio[J].China Environmental Science,1998,18(4):349-352(in Chinese)
• [11]Huang W,Cao L,Ye Z J,et al.Antioxidative responses and bioaccumulation in Japanese flouder larvae and juveniles under chronic mercury exposure[J].Comparative Biochemistry&Physiology,2010,152(1):99-106
• [12]Huang W,Cao L,Liu J,et al.Short-term Mercury exposure affecting the development and antioxidant biomarkers of Japanese flounder embryos and larvae[J].Ecotoxicology and Environmental Safety,2010,73(8):1875-1883
• [13]Chanee B,Maehly A C.Methods in Enzymology[M].New York:Aeademie Press,1995
• [14]焦传珍.铈对镉染毒泥鳅肝胰脏中超氧化物歧化酶和过氧化物酶活性的影响[J].水产科学,2009,28(12):786-788Jiao C Z.The effect of cerium on SOD and POD activities in hepatopancreas of oriental weatherfish(Misgurnus anguillicaudatus)stressed by cadmium[J].Fisheries Science,2009,28(12):786-788(in Chinese)
• [15]Khan H A,Abdelhalim M A K,Al-Ayed M S,et al.Effect of gold nanoparticles on glutathione and malondi-aldehyde levels[J].Saudi Journal of Biological Sciences,2012,19(4):461-464
• [16]Orestis I,Basilios P,Panagiotis T,et al.Effect of moxifloxacin on survival,lipid peroxidation and inflammation in immunosuppressed rats with soft tissue infection caused by stenotrophomonas in liver,lung and heart of rats[J].Saudi Journal of Biological Sciences,2012,19(4):461-464
• [17]Martinez Alvarez R M,Hidalgo M C,Domezain A,et al.Physiological changes of strugeon Acipenser naccarii caused by increasing environmental salinity[J].Journal of Experimental Biology,2002,205:3699-3706
• [18]Allen P.Soft-tissue accumulation of lead in the blue tilapia,Oreochromis aureus(Steindachner),and the modifying effects of cadmium and mercury[J].Biological Trace Element Research,1995,50(3):193-208
• [19]Boyle D,Al-Bairuty G A,Ramsden C S,et al.Subtle alterations in swimming speed distributions of rainbow trout exposed to titanium dioxide nanoparticles are associated with gill rather than brain injury[J].Aquatic Toxicology,2013,126:116-121
• [20]Vensna P,Vesela K.Effects of trifluralin on carp:Biochemical and histological evaluation[J].Ecotoxicology and Environmental Safety,1999,43(2):213-221
• [21]关海红,潘伟志,蔺玉华,等.4种重金属对松浦鲤生长及组织的影响[J].华中农业大学学报,2005,24(1):68-73Guan H H,Pan W Z,Lin Y H,et al.The effect of four heavy metals on Cyprinus carpio Songpu carp's growth and tissues[J].Joural of Hua Zhong Agricultural University,2005,24(1):68-73(in Chinese)