逯南南;宋武昌;王明泉;孙韶华;贾瑞宝;

• 1:山东省城市供排水水质监测中心

摘要(Abstract)：

以斑马鱼为受试生物,通过水质毒性生物监测仪记录行为轨迹,研究了亚致死浓度的马拉硫磷急性暴露下斑马鱼游动行为和群体分布等多项行为参数的变化。结果表明:斑马鱼对环境变化响应快速,游动速度短时间内急剧增大,之后下降再调整稳定至一定范围,变化趋势符合环境压力模型。游泳高度不断增大,暴露1 h后斑马鱼几乎全部集中到水箱上部,与暴露前水平差异显著。通讯行为参数平均距离和分散度在暴露后短时间内减小之后恢复到暴露前水平。通过解析斑马鱼的行为变化可以实现水体有机磷农药突发污染的早期预警。

关键词(KeyWords)： 马拉硫磷;斑马鱼;行为效应;生物监测

Abstract:

Keywords:

基金项目(Foundation): 山东省自然科学基金项目(ZR2014CP019);; 国家水体污染控制与治理科技重大专项“南水北调山东受水区饮用水安全保障技术研究与综合示范”课题(2012ZX07404-003);; 直饮水科技惠民示范工程(2013GS370202)

作者(Author): 逯南南;宋武昌;王明泉;孙韶华;贾瑞宝;

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DOI:

参考文献(References)：

• [1]张祖麟,余刚,洪华生,等.河口水体中有机磷农药的环境行为及其风险影响评价[J].环境科学,2002,23(s):73-78Zhang Z L,Yu G,Hong H S,et al.Environmental behavior and risk assessment of organic phosphorus pesticides at water from Jiulong river estuary[J].Environmental Science,2002,23(s):73-78(in Chinese)
• [2]任宗明,李志良,饶凯锋,等.氰戊菊酯和氯化镉暴露下日本青鳉的行为反应差异[J].生态毒理学报,2008,3(6):563-569Ren Z M,Li Z L,Rao K F,et al.The differences of the behavioral responses of Japanese medaka(Oryzias latipes)in the exposure of fenvalerate and cadmium chloride[J].Asian Journal of Ecotoxicology,2008,3(6):563-569(in Chinese)
• [3]刘勇,付荣恕,任宗明.2种有机磷农药联合胁迫下日本青鳉的逐级行为响应[J].环境科学,2010,31(5):1328-1332Liu Y,Fu R Sh,Ren Z M.Stepwise behavioral responses of the Japanese medaka under the joint stress of two organophosphorus pesticides[J].Environmental Science,2010,31(5):1328-1332(in Chinese)
• [4]黄毅,张金松,韩小波,等.氯化镉和敌敌畏突发胁迫下斑马鱼的行为差异[J].生态毒理学报,2012,7(6):671-676Huang Y,Zhang J S,Han X B,et al.Behavioral differences of zebrafish under sudden stress of dichlorvos and cadmium chloride[J].Asian Journal of Ecotoxicology,2012,7(6):671-676(in Chinese)
• [5]黄毅,张金松,韩小波,等.斑马群体行为变化用于水质在线预警的研究[J].环境科学学报,2014,34(2):387-393Huang Y,Zhang J S,Han X B,et al.Vision-based realtime monitoring on the behavior of zebrafish school[J].Acta Scientiae Circumstantiae,2014,34(2):387-393(in Chinese)
• [6]逯南南,李汝,宋武昌,等.马拉硫磷和百菌清胁迫下斑马鱼行为变化的研究[J].生态毒理学报,2016,11(1):369-374Lu N N,Li R,Song W C,et al.Study on behavioral change of zebrafish exposed to malathion and chlorothalonil[J].Asian Journal of Ecotoxicology,2016,11(1):369-374(in Chinese)
• [7]任宗明,马梅,王子健.饮用水生产中突发性有机磷农药污染事故的在线生物监测[J].给水排水,2006,32(2):17-20Ren Z M,Ma M,Wang Z J.On-line bio-monitoring of organophosphorous pesticides in case of accidental contamination of drinking water[J].Water and Wastewater Engineering,2006,32(2):17-20(in Chinese)
• [8]Parrish J K,Viscidos S V,Grünbaum D.Selforganized fish schools:An examination of emergent properties[J].The Biological Bulletin,2002,202(3):296-305
• [9]Chew F B,Eng H L,Myo T.Vision-based real-time monitoring on the behavior of fish school[C].Yokohama,Japan:MVA 2009 IAPR Conference on Machine Vision Applications,2009
• [10]Kang I J,Juny A M,Mitoshi Y,et a1.Swimming Behavioral Toxicity in Japanese Medaka Exposed to Various Chemicals for Biological Monitoring of Water Quality[M]//Kim Y,Platt V,Gu M B,et al.Atmospheric and Biological Environmental Monitoring.Berlin:Springer,2009:285-293
• [11]Faulk C K,Fuiman L A,Thomas P.Parental exposure to ortho,para-dichlorodiphenyltrichloroethane impairs survival skills of atlantic croaker(Micropogonias undulates)larvae[J].Environmental Toxicology and Chemistry,1999,18(2):254-262
• [12]刘勇,张高生,陈琳琳,等.2种除草剂联合胁迫下日本青鳉的逐级行为响应[J].生态毒理学报,2010,5(1):32-37Liu Y,Zhang G S,Chen L L,et a1.The stepwise behavioral responses of the Japanese medaka under the joint stress of two herbicides[J].Asian Journal of Ecotoxicology,2010,5(1):32-37(in Chinese)
• [13]Ren Z M,Li Z L,Zha J M,et al.The avoidance responses of Daphnia magna to the exposure of organophosphorus pesticides in an on-line biomonitoring system[J].Environmental Modeling&Assessment,2009,14(3):405-410
• [14]赵晓艳,刘丽君,聂湘平,等.利用多物种净水监测仪在线监测水体抗生素药物及有机磷农药[J].环境科学学报,2010,30(1):180-185Zhao X Y,Liu L J,Nie X P,et al.Online biomonitoring for waterborne antibiotics and organophosphorous using the multispecies freshwater biomonitor[J].Acta Scientiae Circumstantiae,2010,30(1):180-185(in Chinese)
• [15]Montesinos T,Perez Munguia S,Valdez F,et al.Disposal cholinesterase biosensor for the detection of pesticides in water miscible organic solvents[J].Analytica Chimica Acta,2001,431(2):231-237
• [16]Fukuda S J,Kang I J,Moroishi J,et al.The application of entropy for detecting behavioral responses in Japanese medaka(Oryzias latipes)exposed to different toxicants[J].Environmental Toxicology,2010,25(5):446-455
• [17]魏渲辉,汝少国,姜明,等.久效磷对美国红鱼鳃Na+/K+-ATP酶活性和超显微结构的影响[J].应用生态学报,2003,14(12):2289-2294Wei X H,Ru S G,Jiang M,et al.Effect of monocrotophos on Na+/K+-ATPase activity and ultramicrostructure of Sciaenops ocellatus branchiae[J].Chinese Journal of E-cology,2003,14(12):2289-2294(in Chinese)
• [18]Roy P K,Munshi J S D.Malathion induced structural and morphometric changes of gills of a fresh water major carp,Cirrhinus mrigala(Ham.)[J].Journal of Environmental Biology,1991,12(1):79-87