碲化镉量子点诱导氧自由基产生的检测Detection of Reactive Oxygen Species Induced by Cadmium Tellurium Quantum Dots
刘娜;赵淑锐;林彤;冯爽;牟颖;韩四海;黄沛力;孙志伟;
摘要(Abstract):
随着量子点(quantum dots,QDs)在生物标记和医学影像等领域的应用日益广泛,其环境暴露量逐渐增加,深入探讨QDs的毒性机制具有重要意义。QDs通过产生活性氧(ROS)诱发毒性效应是目前普遍接受的毒作用模式。为了探讨量子点的毒性与所诱发的ROS的种类和数量的关系,选用碲化镉量子点(CdTe QDs和CdTe/ZnS QDs),利用电子顺磁共振技术(EPR)分别测定了CdTe QDs和CdTe/ZnS QDs在无细胞体系中诱导ROS产生的种类和强度;利用EPR法、紫外可见分光光度法和荧光分光光度法分别测定了4、20和100nmol·mL-1的CdTe QDs和CdTe/ZnS QDs对超氧阴离子(·O2-)和羟基自由基(·OH)产生的促进作用。实验结果显示,CdTe QDs可诱导·O2-的产生;CdTe QDs和CdTe/ZnS QDs对·OH与·O2-的产生有明显的促进作用,且具有剂量-效应关系。研究表明,量子点可诱导和促进ROS产生,不同结构量子点对ROS的诱导和促进作用不同。
关键词(KeyWords): 量子点;羟基自由基;超氧阴离子;电子顺磁共振
基金项目(Foundation): 国家自然科学基金资助项目(31070772);; 苏州市科技计划项目(ZXG0920)
作者(Author): 刘娜;赵淑锐;林彤;冯爽;牟颖;韩四海;黄沛力;孙志伟;
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