刘娜;赵淑锐;林彤;冯爽;牟颖;韩四海;黄沛力;孙志伟;

• 1:首都医科大学公共卫生与家庭医学学院
• 2:首都医科大学医学实验与测试中心现代药学实验与测试室
• 3:浙江大学智能系统与控制研究所分析仪器研究中心工业控制技术国家重点实验室

摘要(Abstract)：

随着量子点(quantum dots,QDs)在生物标记和医学影像等领域的应用日益广泛,其环境暴露量逐渐增加,深入探讨QDs的毒性机制具有重要意义。QDs通过产生活性氧(ROS)诱发毒性效应是目前普遍接受的毒作用模式。为了探讨量子点的毒性与所诱发的ROS的种类和数量的关系,选用碲化镉量子点(CdTe QDs和CdTe/ZnS QDs),利用电子顺磁共振技术(EPR)分别测定了CdTe QDs和CdTe/ZnS QDs在无细胞体系中诱导ROS产生的种类和强度;利用EPR法、紫外可见分光光度法和荧光分光光度法分别测定了4、20和100nmol·mL-1的CdTe QDs和CdTe/ZnS QDs对超氧阴离子(·O2-)和羟基自由基(·OH)产生的促进作用。实验结果显示,CdTe QDs可诱导·O2-的产生;CdTe QDs和CdTe/ZnS QDs对·OH与·O2-的产生有明显的促进作用,且具有剂量-效应关系。研究表明,量子点可诱导和促进ROS产生,不同结构量子点对ROS的诱导和促进作用不同。

关键词(KeyWords)： 量子点;羟基自由基;超氧阴离子;电子顺磁共振

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(31070772);; 苏州市科技计划项目(ZXG0920)

作者(Author): 刘娜;赵淑锐;林彤;冯爽;牟颖;韩四海;黄沛力;孙志伟;

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DOI:

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