聂湘平;鹿金雁;李潇;杨宇峰;

• 1:暨南大学水生生物研究所
• 2:暨南大学水生生物研究所
• 3:暨南大学水生生物研究所
• 4:暨南大学水生生物研究所 广州510632

摘要(Abstract)：

以蛋白核小球藻(Chlorella pyrenoidosa)为实验材料,研究了喹诺酮类药物诺氟沙星(Norfloxacin,NFLX)对小球藻生长及抗氧化酶活性的影响.结果表明,NFLX对小球藻的96hEC50为30.78mg·L-1,属于低毒.NFLX暴露对小球藻谷胱甘肽硫转移酶(GST)和过氧化氢酶(CAT)具有显著影响,当NFLX浓度高于30mg·L-1时,小球藻GST活性受到显著诱导,并随NFLX浓度增加而显著升高,而小球藻CAT活性随NFLX暴露浓度的升高表现出先诱导后抑制现象.NFLX对谷胱甘肽(GSH)和7-乙氧基-异吩唑酮-脱乙基酶(EROD)的影响较弱.在低浓度NFLX暴露下,GST和CAT可作为NFLX暴露的生物标记物.

关键词(KeyWords)： 蛋白核小球藻;诺氟沙星;急性毒性;抗氧化酶活性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(No.40471118);; 国家自然科学基金委与广东省联合基金项目(No.U0633006)

作者(Author): 聂湘平;鹿金雁;李潇;杨宇峰;

Email:

DOI:

参考文献(References)：

• Arriaga A M,Rivera S R,Parra C G,Barro M F,Flores P R,Garcia J E.2000.Antimutagenesis ofβ-carotene to mutationsinduced by quinolone on Salmonella typhimurium[J].Arch MedRes,31:156-161
• Chen J P,Xu L H,Wu Z B,Zhang Y Y,Lin Q S.1999.Molecularecotoxicological indicators of fish intoxicated by Benzo(a)pyrene[J].Chin Environ Sci,19(5):417-420(in Chinese)
• Editorial Board of Analytical Method of Monitoring Water and Wastewaterof Environmental Protection Administration of China.2002.AnalyticalMethod of Monitoring Water and Wastewater(4th)[M].Beijing:China Environmental Science Press.715-721(in Chinese)
• Editorial Board of Manual of Monitoring Aquatic Organisms ofEnvironmental Protection Administration of China.1993.Manualof Monitoring Aquatic Organisms[M].Nanjing:Southeast UniversityPress.192-202(in Chinese)
• Hao Z B,Cang J,Xu Z.2004.Experiment of Plants Physiology[M].Harbin Industry University Press.67-68,113-114(in Chinese)
• Ji C N,Yue Z F,Xie L Q,Zheng W P,Chen P J.2005.Study on simultaneous detection of7tetracyclines residues in aquaticproducts by high performance liquid chromatography[J].Chin JVet Sci Technol,35(10):820-826(in Chinese)
• Li H S.2000.Plant Physio-Biochemistry Experiment Principal andTechnology[M].Beijing:Higher Education Press(in Chinese)
• Li Z X,Leng K L,Li J,Li X C,Wang W F.2001.Thequality of aquatic animal medicines and the supervision ofmedicine residues in aquatic products[J].Mar Fish Res,22(2):76-80(in Chinese)
• Luo X Y,Lin Y N,Liu L Z.2005.Study on the rapiddetermination of4kinds of antibiotcs residues in aquaculture bySPE-HPLC[J].Modern Prev Med,32(3):198-199(in Chinese)
• Peng J L,Yan G A,Sheng G X,Chang L Y,Wang Y,Yang F X.2001.The effects ofα-naphthol on the growth and the antioxidaseactivities of Chlorella Vulgaris[J].Journal of Wuhan University(Natural Science Edition),47(4):449-452(in Chinese)
• Pohl R J,Fouts J R.1980.A rapid method for assaying themetabolism of7-ethoxyresourufin by microsomal subcellular fractions[J].Anal Biochem,107:150-155
• Shen G X,Yan G A,Yu X.1999.The toxic effects of naphthaleneand its derivatives on Chlorella Vulgaris[J].Acta Hydrobio Sin,23(5):460-468(in Chinese)
• Vaccaro E,Giorgi M,Longo V,Mengozzi G,Gervasi P G.2003.Inhibition of cytochrome P450enzymes by enrofloxacin in the seabass(Dicentrarchus labrax)[J].Aquat Toxicol,62:27-33
• Wang J Y,Huo C L,Han G C.2003.Induction of ERODactivity in Paralichthys olivaceus by polychlorinated biphenyl CB-28[J].Acta Oceanol Sin,25(2):35-40(in Chinese)
• Wang R,Liu T Z,Wang T.2006.The fate of antibiotics inenvironment and its ecotoxicology:A review[J].Ecol Sin,26(1):265-270(in Chinese)
• Wang Y,Wang C X,Xu J B.2000.EROD induction of carpliver microsome by PAHs in in-vitro[J].Acta Sci Circums,20:177-180(in Chinese)
• Xu J B,Wang Y,Zhang L,Wang C X.2003.Effects of ninenitrobenzenes on enzymic activity of EROD in carp hepatic MicrosomalSystem[J].Res Environ Sci,16(1):43-45(in Chinese)
• Yang Z Q,Dong B,Wu J C.2004.Sensitivity of Chlorellavulgaris to metribuzin Puma and alachlor[J].Chin J App Eco,15(9):1621-1625(in Chinese)
• Zhou Q X,Kong F X,Zhu L.2004.Ecotoxicology[M].Beijing:Science Press.55-110
• 陈加平,徐立红,吴振斌,张甬元,林群声.1999.苯并(a)芘致毒的鱼的分子生态毒理学指标研究[J].中国环境科学,19(5):417-420
• 国家环保局《水和废水监测分析方法》编委会.2002.水和废水监测分析方法(第四版)[M].北京:中国环境科学出版社.715-721
• 国家环保局《水生生物监测手册》编委会.1993.水生生物监测手册[M].南京:东南大学出版社.192-202
• 郝再彬,苍晶,徐仲.2004.植物生理实验[M].哈尔滨:哈尔滨工业大学出版社.67-68,113-114
• 吉彩霓,岳振峰,谢丽琪,郑卫平,陈佩金.2005.高效液相色谱法同时检测水产品中7种四环素类抗生素残留的研究[J].中国兽医科技,35(10):820-826
• 李合生.2000.植物生理生化实验原理和技术[M].北京:高等教育出版社
• 李兆新,冷凯良,李健,李晓川,王维芬.2001.我国渔药质量状况及水产品中渔药残留监控[J].海洋水产研究,22(2):76-80
• 罗晓燕,林玉娜,刘莉治.2005.SPE-HPLC法同时测定水产品中四种抗生素残留的研究[J].现代预防医学,32(3):198-199
• 彭金良,严国安,沈国兴,常利英,王昀,杨方星.2001.α-萘酚胁迫对普通小球藻生长及抗氧化酶活性的影响[J].武汉大学学报(理学版),47(4):449-452
• 沈国兴,严国安,余新.1999.萘及其衍生物对普通小球藻的毒性效应[J].水生生物学报,23(5):460-468
• 王菊英,霍传林,韩庚辰.2003.多氯联苯CB-28对牙鲆肝脏中EROD活性的诱导作用研究[J].海洋学报,25(2):35-40
• 王冉,刘铁铮,王恬.2006.抗生素在环境中的转归及其生态毒性[J].生态学报,26(1):265-270
• 王咏,王春霞,徐静波.2000.多环芳烃化合物对鲤鱼肝微粒体EROD的体外诱导[J].环境科学学报,20:177-180
• 徐镜波,王咏,张蕾,王春霞.2003.9种硝基苯对鱼肝微粒体EROD活性的影响[J].环境科学研究,16(1):43-45
• 杨志强,董波,吴进才.2004.普通小球藻对嗪草酮、骠马和甲草胺的敏感性研究[J].应用生态学报,15(9):1621-1625
• 周启星,孔繁翔,朱琳.2004.生态毒理学[M].北京:科学出版社.55-110