肖国强;徐晓宇;蔡文君;付聪;吴曲;丁书茂;袁均林;袭著革;杨旭;

• 1:华中师范大学生命科学学院环境科学实验室
• 2:遗传调控与整合生物学湖北省重点实验室
• 3:军事医学科学院卫生学环境医学研究所

摘要(Abstract)：

为探讨纳米TiO2对肝、肾细胞DNA的损伤效应,实验制备了锐钛矿型纳米TiO2颗粒(20～100nm),并采用不同浓度的纳米TiO(20、0.1、0.2、0.4、0.8mg·mL-1)对25只昆明雄性小鼠进行染毒,5d后用单细胞凝胶电泳技术检测小鼠肝、肾细胞DNA的损伤程度.结果表明,随着纳米TiO2染毒浓度的升高,小鼠肝、肾细胞尾部DNA百分率(Tail DNA%)和尾矩(Tail Moment)均呈逐渐升高趋势;对于肝细胞,0.1mg·mL-1组Tail DNA%及Tail Moment与对照组无显著差异(p>0.05),≥0.2mg·mL-1组则显著升高(p<0.05或p<0.01);与肝细胞相比,肾细胞Tai lDNA%及Tail Moment变化更为显著,0.1mg·mL-1组即与对照组存在极显著差异(p<0.01).以上结果表明,纳米TiO2染毒可导致小鼠肝、肾细胞DNA损伤,且随染毒浓度的增加,损伤逐渐加重,呈一定的剂量-效应关系;与肝细胞相比,肾细胞对纳米TiO2更为敏感,较低浓度染毒即可造成DNA损伤.

关键词(KeyWords)： 纳米TiO2;DNA损伤;单细胞凝胶电泳

Abstract:

Keywords:

基金项目(Foundation): 国家高技术研究发展规划(863)项目(No.2006AA03Z330)

作者(Author): 肖国强;徐晓宇;蔡文君;付聪;吴曲;丁书茂;袁均林;袭著革;杨旭;

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DOI:

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