应用流式细胞分析技术研究溴氯海因对近头状伪蹄形藻的毒性作用Study on Toxic Effect of Bromo-chloro-dimethylhydantoin on Pseudokirchneriella subcapitata by Flow Cytometry
李万芳;史蕤;王福鑫;魏金锋;王爱平;靳洪涛;
摘要(Abstract):
实验采用流式细胞术的检测分析方法,探究溴氯海因(BCDMH)对常见淡水藻类近头状伪蹄形藻的作用。研究结果显示BCDMH对近头状伪蹄形藻的生长抑制作用呈明显的浓度依赖型变化。根据不同浓度细胞生长抑制的百分率计算,24、48和72hBCDMH对近头状伪蹄形藻生长抑制作用的EC50分别为3.64、3.21和2.56mg·mL-1。生长抑制作用的无可观察效应浓度(NOEC)为0.31mg·mL-1。伴随着生长抑制作用,近头状伪蹄形藻细胞的活性氧(ROS)含量、酯酶活性及细胞膜完整性均发生了不同程度的改变,NOEC分别为0.16、0.31和1.3mg·mL-1。其中ROS含量的升高对BCDMH浓度的变化最为敏感,其次是酯酶活性的变化,细胞膜破损仅在5.0mg·mL-1和2.5mg·mL-1浓度组出现。结合本次研究结果,在日常使用BCDMH时,需要综合考虑使用环境和用途等因素,在不影响使用效果的前提下,应尽可能地降低其使用浓度。
关键词(KeyWords): 溴氯海因(BCDMH);近头状伪蹄形藻;流式细胞术(FCM);酯酶;活性氧(ROS)
基金项目(Foundation): “重大新药创制”科技重大专项(No.2008ZX09305-001)
作者(Author): 李万芳;史蕤;王福鑫;魏金锋;王爱平;靳洪涛;
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