王法琦;刘薇;金一和;于文广;刘晓晖;刘力;

• 1:大连理工大学环境与生命学院
• 2:中国医科大学公共卫生学院

摘要(Abstract)：

为了阐明PFOS导致新生儿死亡的可能原因和机制,探讨PFOS致死与神经系统发育的相互联系,利用基因芯片技术,观察了经PFOS饲料染毒后出生1和7d大鼠脑组织基因表达情况,通过基因组学(Gene Ontology)和生物路径(Pathway)对差异基因的功能和相互联系进行分析.结果显示,PFOS染毒后,出生1、7d的大鼠脑皮质组织分别有864、642条基因发生差异表达,差异基因涉及的与PFOS致死相关的生物过程包括中枢神经系统发育、血循环系统、刺激应答、骨骼发育、氧化应激、心脏功能以及pH值调节等.结果表明,PFOS导致的出生早期死亡与神经系统发育有关,PFOS可能通过改变脑组织血氧平衡,影响正常的中枢神经系统功能和发育过程而使新生儿的存活率下降.

关键词(KeyWords)： 全氟辛烷磺酸;出生早期死亡;基因芯片;发育神经毒性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(No.30771772)

作者(Author): 王法琦;刘薇;金一和;于文广;刘晓晖;刘力;

Email:

参考文献(References)：

• Abbott B D,Wolf C J,Das K P,Zehr R D,Schmid J E,Lindstrom A B,Strynar M J,Lau C.2009.Developmentaltoxicity of perfluorooctane sulfonate(PFOS)is not dependent onexpression of peroxisome proliferators activated receptor-alpha(PPARγ)in the mouse[J].Reproductive Toxicology27(3-4):258-265
• Austin M E,Kasturi B S,Barber M,Kannan K,MohanKumar PS,MohanKumar S M.2003.Neuroendocrine effects ofperfluorooctane sulfonate in rats[J].Environmental HealthPerspectives,111(12):1485-1489
• Borg D,Bogdanska J,Sundstrm M,Nobel S,Hkansson H,Bergman A,DePierre J,Halldin K,Bergstrom U.2009.Perinataltissue distribution of perfluorooctane sulphonate(PFOS)in mice[J].Toxicology Letters,189(1):147
• Butenhoff J L,Ehresman D J,Chang S C,Parker G A,StumpD G.2009.Gestational and lactational exposure to potassiumperfluorooctanesulfonate(K+PFOS)in rats:developmental neurotoxicity[J].Reproductive Toxicology,27(3-4):319-330
• Calafat A M,Needham L L,Kuklenyik Z,Reidy J A,Tully JS,Aguilar-Villalobos M,Naeher L P.2006.Perfluorinatedchemicals in selected residents of the American continent[J].Chemosphere,25(3):490-496
• Du Y,Shi X,Liu C,Yu K,Zhou B.2009.Chronic effects ofwater-borne PFOS exposure on growth,survival and hepatotoxicityin zebrafish:a partial life-cycle test[J].Chemosphere,74(5):723-729
• Dutra F,Quintans G,Banchero G.2007.Lesions in the centralnervous system associated with perinatal lamb mortality[J].Australian Veterinary Journal,85(10):405-413
• Fuentes S,Colomina M T,Rodriguez J,Vicens P,Domingo JL.2006.Interactions in developmental toxicology:concurrentexposure to perfluorooctane sulfonate(PFOS)and stress in pregnantmice[J].Toxicology Letters.164(1):81-89
• Grasty R C,Bjork J A,Wallace K B,Wolf D C,Lau C S,Rogers J M.2005.Effects of prenatal perfluorooctane sulfonate(PFOS)exposure on lung maturation in the perinatal rat[J].Birth Defects Research.Part B,Developmental ReproductiveToxicology,74(5):405-416
• Grasty R C,Wolf D C,Grey E,Lau C S,Rogers J M.2003.Prenatal window of susceptibility to perfluorooctane sulfonate-induced neonatal mortality in the Sprague-Dawley rat[J].BirthDefects Research.Part B,Developmental Reproductive Toxicology,68(6):465-471
• Haughey K G.1973.Vascular abnormalities in the central nervoussystem associated with perinatal lamb mortality.2.Association ofthe abnormalities with recognised lesions[J].Australian VeterinaryJournal,49(1):9-15
• Hu C L,Zhong L F.2006.Progress of toxicological study onPFOS and PFOA[J].Chinese Journal of Industrial Medicine,19(6):1244-1247(in Chinese)
• Johansson N,Fredriksson A,Eriksson P.2008.Neonatal exposureto perfluorooctane sulfonate(PFOS)and perfluorooctanoic acid(PFOA)causes neurobehavioural defects in adult mice[J].NeuroToxicology,29(1):160-169
• Krφvel A V,Sφfteland L,Torstensen B,Olsvik P A.2008.Transcriptional effects of PFOS in isolated hepatocytes fromAtlantic salmon Salmo salar L.[J].Comparative Biochemistryand Physiology.Part C,Toxicology&Pharmacology,148(1):14-22
• Lau C,Thibodeaux J R,Hanson R G,Rogers J M,Grey B E,Stanton M E,Butenhoff J L,Stevenson L A.2003.Exposure toperfluorooctane sulfonate during pregnancy in Ratand mouse.II:Postnatal evaluation[J].Toxicological Sciences,74(2):382-392
• Liu L,Jin Y H,Wang L,Yu H Y,Liu W,Yu Q L,WangK,Liu B,Wang J.2009.Effects of prenatal and postnatalperfluorooctane sulfonate exposure on IGF-I,IGF-IR and IGFBP-2mRNA levels in frontal cortex of rat pups[J].Asian Journalof Ecotoxicology,4(2):190-196(in Chinese)
• Luebker D J,Case M,York R G,Moore J A,Hansen K J,Butenhoff J L.2005a.Two-generation reproduction and cross-foster studies of perfluorooctanesulfonate(PFOS)in rats[J].Toxicology,215(1-2):126-148
• Luebker D J,York R G,Hansen K J,Moore J A,Butenhoff JL.2005b.Neonatal mortality from in utero exposure toperfluorooctanesulfonate(PFOS)in Sprague-Dawley rats:dose-response,and biochemical and pharamacokinetic parameters[J].Toxicology,215(1-2):149-169
• Shi X,Du Y,Lam P K,Wu R S,Zhou B.2008.Developmentaltoxicity and alteration of gene expression in zebrafish embryosexposed to PFOS[J].Toxicology and Applied Pharmacology,230(1):23-32
• Slotkin T A,MacKillop E A,Melnick R L,Thayer K A,Seidler F J.2008.Developmental neurotoxicity of perfluorinatedchemicals modeled in vitro[J].Environmental Health Perspectives,116(6):716-722
• Smithwick M,Muir D C,Mabury S A,Solomon K R,Martin JW,Sonne C,Born E W,Letcher R J,Dietz R.2005.Perflouroalkyl contaminants in liver tissue from East Greenlandpolar bears(Ursus maritimus)[J].Environmental Toxicolgy&Chemistry,24(4):981-986
• White H,Pieper C,Schmader K.1998.The association ofweight change in Alzheimer’s disease with severity of diseaseand mortality:A longitudinal analysis[J].Journal of theAmerican Geriatrics Society,46(10):1223-1227
• Wilson W R,Geraci J E,Danielson G K,Thompson R L,Spittell J A,Washington J R,Giuliani E R.1978.Anticoagulanttherapy and central nervous system complications in patients withprosthetic valve endocarditis[J].Circulation,57(5):1004-1007
• Yahia D,Tsukuba C,Yoshida M,Sato I,Tsuda S.2008.Neonatal death of mice treated with perfluorooctane sulfonate[J].Journal of Toxicological Sciences,33(2):219-226
• Yeung L W Y,Guruge K S,Yamanaka N,Miyazaki S,Lam PK S.2007.Differential expression of chicken hepatic genesresponsive to PFOA and PFOS[J].Toxicology,237(1-3):111-125
• Yu W G,Liu W,Jin Y H,Liu X H,Wang F Q,Liu L,Nakayama S F.2009.Prenatal and postnatal impact ofperfluorooctane sulfonate(PFOS)on rat development:A cross-foster study on chemical burden and thyroid hormone system[J].Environmental Science&Technology,43(21):8416-8422
• 胡存丽,仲来福.2006.全氟辛烷磺酸和全氟辛酸毒理学研究进展[J].中国工业医学杂志,19(6):1244-1247
• 刘利,金一和,王烈,于红瑶,刘薇,于棋麟,王柯,刘冰,王静.2009.出生前后全氟辛烷璜酸(PFOS)暴露额叶皮质中IGF-I、IGF-IR和IGFBP-2mRNA水平的影响[J].生态毒理学报,4(2):190-196