陈文婕;戴红;陈敏;吴茂聪;刘鹏;胡月玲;

• 1:内蒙古医学院公共卫生学院环境卫生职业医学教研室
• 2:大连医科大学公共卫生学院食品与营养学教研室
• 3:大连医科大学公共卫生学院劳动卫生环境卫生教研室

摘要(Abstract)：

为研究邻苯二甲酸二乙基己酯(DEHP)对小鼠肝脏的毒性及脂质过氧化损伤作用机制,选择昆明4周龄小鼠80只,雌雄各半,随机分为4组。经食饵连续自然给食染毒,于染毒第4周末处死。测量小鼠肝脏和体重的变化,测定不同DEHP染毒剂量组小鼠的血液、肝脏中谷胱甘肽过氧化物酶(GPX)、过氧化氢(H2O2)和丙二醛(MDA),超氧化物歧化酶(SOD)的变化。将实验数据进行ANO-VA分析处理。结果表明,随着染毒剂量的增加,小鼠体重逐渐减少(p<0.01),高剂量组肝脏器系数明显上升(p<0.01)。苏木精-伊红染色法(简称HE染色)可见高剂量组肝脏组织有明显损伤。与对照组相比,DEHP3个剂量染毒组小鼠血液(75mg·kg-1组除外)及肝脏中GPX活性降低(p<0.05,p<0.01),H2O2含量增加(p<0.05,p<0.01);肝组织中(75mg·kg-1组除外)SOD活性降低(p<0.05,p<0.01),MDA含量增加(p<0.01)。以上结果说明DEHP对小鼠肝脏的毒性作用机制可能为脂质过氧化反应。

关键词(KeyWords)： DEHP;肝脏;脂质过氧化损伤

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 陈文婕;戴红;陈敏;吴茂聪;刘鹏;胡月玲;

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