斑马鱼p53基因结构比对分析及其在生态毒理学中的应用Structure Alignments of Zebrafish p53 Gene and Its Application in Ecotoxicology
顾颖;李延;陈蔚丰;尹大强;赵庆顺;
摘要(Abstract)：
斑马鱼p53基因由11个外显子和10个内含子组成,外显子1和外显子11部分序列不参与编码蛋白质．其 cDNA核苷酸序列共2089bp,包含一个1122 bp的开放阅读框(Open Reading Frame,ORF)(44 bp-1165 bp),这个 ORF编码373个氨基酸．另外在3’端有924 bp是非翻译区(3’-UTR),5’端同样也存在43 bp的非翻译区(5’- UTR);12种脊椎动物p53氨基酸序列的保守性分析结果表明,在一些特殊的区域,12种氨基酸序列表现出了很高的保守性,这些区域和p53的抑癌功能密切相关．研究结果预示斑马鱼p53基因是一种理想的环境致突变物研究分子模型,在生态毒理学研究中具有良好的应用前景．
关键词(KeyWords)： 斑马鱼;p53基因;环境致突变物;生态毒理学
基金项目(Foundation): 国家自然科学基金项目(No．20577022,20377022)
作者(Author): 顾颖;李延;陈蔚丰;尹大强;赵庆顺;
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