硫化镉量子点对人胚肝细胞L-02的毒性研究Cadmium Sulfide Quantum Dots Induce Cytotoxicity in Human Embryo Liver Cells
梁丽君;张静姝;郭昌胜;刘克明;柏杉山;王春花;王玉秋;
摘要(Abstract)：
采用乳化液膜法自组合成硫化镉量子点(CdS quantum dots,CdS QDs),探讨CdS QDs的体外毒性作用及可能的作用机制.选用人胚肝细胞(L-02)作为细胞模型,采用不同浓度的CdS QDs(0.00、1.25、2.50、5.00、10.00、20.00、40.00μg·mL-1)对L-02细胞进行染毒.24h后,检测细胞内乳酸脱氢酶(LDH)释放量、谷胱甘肽(GSH)含量和超氧化物歧化酶(SOD)活力,并比较加入抗氧化剂N-乙酞半胱氨酸(NAC)后细胞存活率的变化,同时测定了细胞内外的镉离子浓度.结果表明,与空白对照组相比,CdS QDs单独染毒组细胞存活率显著降低(p<0.05或p<0.01);加入抗氧化剂NAC后,10.00、20.00、40.00μg·mL-1染毒组细胞存活率与单独染毒组相比显著上升(p<0.01).CdS QDs浓度为5.00μg·mL-1时,细胞内Cd2+的浓度略高于细胞外Cd2+的浓度,在其他浓度下,细胞外Cd2+的浓度均显著高于细胞内Cd2+的浓度.当作用浓度上升至10.00μg·mL-1时,人胚肝细胞内LDH含量显著增加,且随着作用剂量的升高,LDH含量逐渐增加.与空白对照组相比,40.00μg·mL-1CdS QDs染毒组SOD活力和20.00μg·mL-1CdS QDs染毒组GSH含量均显著降低(p<0.05).Cd2+易透过L-02细胞的细胞膜而进入细胞内,从而造成细胞损伤.氧化损伤可能是CdSQDs对L-02细胞毒性作用的机制之一.
关键词(KeyWords)： 硫化镉量子点;L-02细胞;细胞毒性;氧化损伤
基金项目(Foundation): 教育部博士点基金(No.200800550011);; 天津市自然科学基金面上项目(No.2005KYZ45)
作者(Author): 梁丽君;张静姝;郭昌胜;刘克明;柏杉山;王春花;王玉秋;
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参考文献(References)：
- Akerman M E,Chan W C W,Laakkonen P,Bhatia S N,Ruoslahti E.2002.Nanocrystal targeting in vivo[J].Proceedingof the National Academy of Sciences of the United States ofAmerica,99(20):12617-12621
- Bai S S,Li Q Z,Liu K M,Zhang J Z,Liu L,Gao J K,Wang Y Q.2008.Comparative study of genotoxicity in miceexposed to cadmium sulfide quantum dots and normal cadmiumsulfide[J].Asian Journal of Ecotoxicology,3(1):59-64(inChinese)
- Chan W H,Shiao N H.2008.Cytotoxic effect of CdSequantum dots on mouse embryonic development[J].ActaPharmacologica Sinica,29(2):259-266
- Chithrani B D,Ghazani A A,Chan W C W.2006.Determiningthe size and shape dependence of gold nanoparticle uptake into mammalian cells[J].Nano Letters,6(4):662-66
- Cho S J,Maysinger D,Jain M,R觟der B,Hackbarth S,WinnikF M.2007.Long-term exposure to CdTe quantum dots causesfunctional impairments in live cells[J].Langmuir,23:1974-1980
- Choi A O,Cho S J,Desbarats J,Lovric J,Maysinger D.2007.Quantum dot-induced cell death involves Fas upregulation andlipid peroxidation in human neuroblastoma cells[J].Journal ofNanobiotechnology,5(1):1-13
- Derfus A M,Chan W C W,Bhatia S N.2004.Probing thecytotoxicity of semiconductor quantum dots[J].Nano Letters,4(1):11-18
- Gao X H,Cui Y Y,Levenson R M,Chung L W K,Nie S M.2004.In vivo cancer targeting and imaging with semiconductorquantum dots[J].Nature Biotechnology,22(8):969-976
- Green M,Howman E.2005.Semiconductor quantum dots andfree radical induced DNA nicking[J].Chemical Communications,(1):121-123
- Hayashi A,Nakanishi T,Kunisawa J,Kondoh M,Imazu S,Tsutsumi Y,Tanaka K,Fujiwara H,Hamaoka T,Mayumi T.1999.A novel vaccine delivery system using immunopotentiatingfusogenic liposomes[J].Biochemical Biophysical ResearchCommunications,261(3):824-828
- Hoshino A,Fujioka K,Oku T,Suga M,Sasaki Y F,Ohta T,Yasuhara M,Suzuki K,Yamamoto K.2004.Physicochemicalproperties and cellular toxicity of nanocrystal quantum dotsdepend on their surface modification[J].Nano Letters,4(11):2163-2169
- Hoshino A,Nagao T,Nakasuga A,Ishida-Okawara A,SuzukiK,Yasuhara M,Yamamoto K.2007.Nanocrystal quantum dot-conjugated anti-myeloperoxidase antibody as the detector ofactivated neutrophils[J].IEEE Transactions on NanoBioscience,6(4):341-345
- Leroueil P R,Hong S Y,Mecke A,Baker J R,Orr B G,HollM M B.2007.Nanoparticle interaction with biological membranes:does nanotechnology present a janus face[J].Accounts ofChemical Research,40(5):335-342
- Li K G,Chen J T,Bai S S,Wen X,Song S Y,Yu Q,Li J,Wang Y Q.2009.Intracellular oxidative stress and cadmium ionsrelease induce cytotoxicity of unmodified cadmium sulfidequantum dots[J].Toxicology in Vitro(,23):1007-1013
- Lovric'J,Cho S J,Winnik F M,Maysinger D.2005.Unmodified cadmium telluride quantum dots induce reactiveoxygen species formation leading to multiple organelle damageand cell death[J].Chemistry&Biology,12(11),1227-1234
- Shekar S N,Banerjee T,Biswas A.2006.Hypotheses on theeffect of cadmium on glutathione content of red blood corpuscles[J].Twin Research and Human Genetics,9(1):73-75
- Tsay J M,Michalet X.2005.New light on quantum dotcytotoxicity[J].Chemistry&Biology,12(11):1159-1161
- Yu Q,Li K G,Wen X,Li J,Wang Y Q.2009.Thecytotoxicity mechanism of unmodified cadmium sulfide quantumdots[J].Asian Journal of Ecotoxicology,4(4):488-493(inChinese)
- 柏杉山,李清钊,刘克明,张静姝,刘璐,高军凯,王玉秋.2008.硫化镉量子点与常规硫化镉对小鼠遗传毒性的比较研究[J].生态毒理学报,3(1):59-64
- 余强,李坤刚,文兴,李君,王玉秋.2009.纳米硫化镉量子点细胞毒性作用机制[J].生态毒理学报,4(4):488-493