王帅;王玥;梁英;郑立;孙承君;鞠鹏;

• 1:自然资源部第一海洋研究所海洋生态环境科学与技术重点实验室
• 2:中国海洋大学海水养殖教育部重点实验室
• 3:青岛海洋科学与技术国家实验室海洋国家实验室海洋生态与环境科学功能实验室

摘要(Abstract)：

海洋微塑料作为一类新型环境污染物已经成为全球性环境问题。运用水样叶绿素荧光仪(Water-PAM)研究了不同浓度软聚氯乙烯(polyvinyl chloride, PVC)和硬PVC浸出液(0、50、100和200 g·L~(-1))胁迫下球等鞭金藻(Isochrysis galbana)、中肋骨条藻(Skeletonema costatum)和小球藻(Chlorella sp.)3种海洋微藻叶绿素荧光特性,包括PSⅡ最大量子产量(F_v/F_m)、PSⅡ实际量子产量(Yield)、光化学淬灭(qP)和PSⅡ最大相对电子传递速率(rETR_(max))的变化,同时检测微藻的生长状况。结果表明,球等鞭金藻(金藻门)和中肋骨条藻(硅藻门)的4项光合作用参数在软PVC浸出液胁迫的96 h内均显著下降,且随软PVC浸出液浓度增加,下降幅度增大,在实验后期(48～96 h)达到最小值,表现为抑制作用。96 h时,球等鞭金藻和中肋骨条藻的细胞密度均在软PVC浸出液200 g·L~(-1)处理组达到最小值,分别占对照组的3.20%和11.90%。硬PVC浸出液胁迫对球等鞭金藻和中肋骨条藻的4项光合作用参数具有显著影响(P<0.05),球等鞭金藻的4项参数均显著高于对照组。中肋骨条藻的相关光合作用参数均显著低于对照组。72 h时球等鞭金藻和中肋骨条藻的细胞密度分别在硬PVC浸出液100 g·L-1和200 g·L~(-1)处理组达到最小值,为对照组的67.90%和82.50%,表现为抑制作用。小球藻(绿藻门)的各光合作用参数和细胞密度在软PVC和硬PVC浸出液胁迫的96 h内未产生显著变化。该研究证实PVC浸出液对微藻光合系统具有干扰作用,对海洋生态系统具有潜在风险。

关键词(KeyWords)： PVC浸出液;球等鞭金藻;中肋骨条藻;小球藻;光合作用参数

Abstract:

Keywords:

基金项目(Foundation): 中央级公益性科研院所基本科研业务费专项资金资助项目(2017Q09);; 国家自然科学基金资助项目(41806201,41776176);; 国家自然科学基金委员会-山东省人民政府海洋科学研究中心联合基金项目(U1606404);; 国家海洋局双边海洋国际事务合作项目(QY0518011);; 青岛海洋科学与技术试点国家实验室海洋生态与环境科学功能实验室开放基金资助项目(LMEES201802)

作者(Author): 王帅;王玥;梁英;郑立;孙承君;鞠鹏;

Email:

DOI:

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