付倩;杨垒滟;赖金龙;陶宗娅;吴国;罗学刚;

• 1:四川师范大学生命科学学院
• 2:西南科技大学生物质材料教育部工程研究中心

摘要(Abstract)：

采用改良水培法培养蚕豆幼苗至2片真叶,置于含铯(ρ(Cs+)8.24(CK)~200 mg·L~(-1))的营养液中处理7 d、14 d、21 d后取样。采用差速离心法分离亚细胞组分,采用原子吸收分光光度法测定根、茎、叶及各亚细胞组分的Cs+含量,分析蚕豆幼苗对Cs+的吸收蓄积及亚细胞分布特点,研究蚕豆对Cs+的富集转运特征及耐受机理。结果显示:蚕豆3种器官对Cs+的蓄积量为根>叶>茎,根对Cs+的蓄积量占总量的65.13%~83.17%,最高(ρ(Cs+)200 mg·L~(-1)时)达到518.40 mg·kg~(-1)FW(7 d)、1 949.74 mg·kg~(-1)FW(14 d)和3 614.03 mg·kg~(-1)FW(21 d);蚕豆根、茎、叶中Cs+的亚细胞分布主要集中在细胞壁和可溶性组分中,Cs+相对含量分别达到75.84%~99.06%(根)、79.06%~100%(茎)、82.95%~100%(叶);细胞核、前质体、叶绿体和线粒体等细胞器仅含少量的Cs+(<25%)。结果表明,蚕豆根、茎、叶细胞主要通过阻滞作用,将Cs+结合固定在细胞壁,并将进入细胞质基质的一部分Cs+转运到液泡内,暂时或"永久性"存贮,有效降低了细胞器、胞质溶胶(cytosol)及内含物中的Cs+含量,极大地减缓了Cs+对细胞器的功能性损伤,这是短期内蚕豆未表现出明显中毒症状的原因,也是蚕豆耐受Cs+胁迫的重要机理之一。

关键词(KeyWords)： 铯;蚕豆;蓄积;亚细胞分布;耐受性

Abstract:

Keywords:

基金项目(Foundation): 国家核设施退役及放射性废物治理科研重点项目(14ZG6101);; 四川省教育厅理工科重点课题(14ZA0030);四川省教育厅大学生创新创业训练计划项目(201410636040);; 四川省生物质改性材料工程技术研究中心(西南科技大学)开放课题(12zxbk03);; 四川师范大学实验技术项目(SYJS2014-07)

作者(Author): 付倩;杨垒滟;赖金龙;陶宗娅;吴国;罗学刚;

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