全氟十二烷酸(PFDoA)慢性暴露干扰大鼠肝脏磷酸化蛋白质水平Chronic Exposure of PFDo A Affect the Phosphorylation Levels of Proteins in Rat Liver
张红霞;崔瑞娜;戴家银;
摘要(Abstract):
全氟烷基类化合物(PFASs)是一类新型持久性有机污染物,因其独特的理化性质被广泛应用于工业和商业领域,对生物体具有一定的毒性作用。为探究全氟十二烷酸(PFDo A)致肝脏毒性作用机制,选择雄性大鼠为受试生物,采用2-DE蛋白质组学技术与Pro Q Diamond dye磷酸化蛋白染色结合的方法初步研究了不同剂量PFDo A暴露110 d后大鼠肝脏蛋白磷酸化水平的变化。结果表明,30个磷酸化蛋白表达水平在PFDo A处理后发生显著变化,其中,经过MALDI-TOF/TOF质谱分析,成功鉴定18个蛋白点。经过生物信息学分析,发现这些蛋白主要涉及糖脂代谢、氨基酸代谢、应激防御及电子传递等途径。以上结果有助于进一步从翻译后修饰水平了解PFDo A的肝脏毒性作用。
关键词(KeyWords): PFDo A;大鼠;肝脏毒性;磷酸化蛋白;Pro-Q Diamond
基金项目(Foundation): 国家自然科学基金(31320103915,21377128)
作者(Author): 张红霞;崔瑞娜;戴家银;
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DOI:
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