张红霞;崔瑞娜;戴家银;

• 1:中国科学院动物研究所中国科学院动物生态与保护生物学重点实验室

摘要(Abstract)：

全氟烷基类化合物(PFASs)是一类新型持久性有机污染物,因其独特的理化性质被广泛应用于工业和商业领域,对生物体具有一定的毒性作用。为探究全氟十二烷酸(PFDo A)致肝脏毒性作用机制,选择雄性大鼠为受试生物,采用2-DE蛋白质组学技术与Pro Q Diamond dye磷酸化蛋白染色结合的方法初步研究了不同剂量PFDo A暴露110 d后大鼠肝脏蛋白磷酸化水平的变化。结果表明,30个磷酸化蛋白表达水平在PFDo A处理后发生显著变化,其中,经过MALDI-TOF/TOF质谱分析,成功鉴定18个蛋白点。经过生物信息学分析,发现这些蛋白主要涉及糖脂代谢、氨基酸代谢、应激防御及电子传递等途径。以上结果有助于进一步从翻译后修饰水平了解PFDo A的肝脏毒性作用。

关键词(KeyWords)： PFDo A;大鼠;肝脏毒性;磷酸化蛋白;Pro-Q Diamond

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31320103915,21377128)

作者(Author): 张红霞;崔瑞娜;戴家银;

Email:

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