赵学平;王彦华;吴声敢;俞瑞鲜;吴长兴;陈丽萍;苍涛;王强;

• 1:浙江省农业科学院农产品质量标准研究所浙江省植物有害生物防控重点实验室&省部共建国家重点实验室培育基地农业部农药残留检测重点实验室

摘要(Abstract)：

为了评价农药对土壤无脊椎动物种群的生态风险,采用OECD标准滤纸法和人工土壤法测定了10种常用农药对蚯蚓(Eisenia foetida)的急性毒性效应。采用滤纸法,48h测定结果表明,4种氨基甲酸酯类(异丙威、甲萘威、速灭威和丁硫克百威)和2种有机磷类(毒死蜱和哒嗪硫磷)农药对蚯蚓的急性毒性(其LC50值为3.50(2.77～4.44)～72.42(59.58～88.05)μg·cm-2)明显高于3种昆虫生长调节剂(噻嗪酮、虫酰肼和呋喃虫酰肼)和吡蚜酮对蚯蚓的急性毒性(其LC50值>629.1μg·cm-2)。采用人工土壤法,14d测定结果表明,上述4种氨基甲酸酯类农药对蚯蚓的急性毒性(其LC50值为59.61(55.13～64.44)～134.1(127.0～141.5)mg·kg-1)明显高于上述2种有机磷类农药和其他农药对蚯蚓的急性毒性(其LC50值为193.0(180.1～206.8)～386.4(359.7～414.2)mg·kg-1)。上述结果表明,不同类型的农药对蚯蚓的毒性存在较大差异,且同一类型的不同农药品种对蚯蚓的毒性也存在较大差异。总体来看,氨基甲酸酯类农药比其他类型的农药对蚯蚓具有更高的毒性。根据《化学农药环境安全评价试验准则》,本研究采用人工土壤法测定的所有农药对蚯蚓均为低毒级。

关键词(KeyWords)： 有机磷类农药;氨基甲酸酯类农药;赤子爱胜蚓;急性毒性

Abstract:

Keywords:

基金项目(Foundation): 科技部国际合作项目(S2010GR0905),科技部863项目(2011AA100806);; 浙江省农业科学院与中国科学院合作项目;; 浙江省重大科技专项(2006C12023)

作者(Author): 赵学平;王彦华;吴声敢;俞瑞鲜;吴长兴;陈丽萍;苍涛;王强;

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参考文献(References)：

• [1]Cortez J,BouchéM.Decomposition of mediterranean leaf litters by Nicodrilus meridionalis(Lumbricidae)in laboratory and field experiments[J].Soil Biology and Biochemistry,2001,33(15):2023-2035
• [2]McInerney M,Little D J,Bolger T.Effect of earthworm cast for-mation on the stabilization of organic matter in fine soil fractions[J].European Journal of Soil Biology,2001,37(4):251-254
• [3]Dimitrova N,Dimitrov S,Georgieva D,et al.Elimination kinetic model for organic chemicals in earthworms[J].Science of the Total Environment,2010,408(18):3787-3793
• [4]Holmstrup M,Srensen J G,Overgaard J,et al.Body metal concentrations and glycogen reserves in earthworms(Den-drobaena octaedra)from contaminated and uncontaminated forest soil[J].Environmental Pollution,2011,159(1):190-197
• [5]Campiche S,Becker-van Slooten K,Ridreau C,et al.Effects of insect growth regulators on the nontarget soil arthropod Folsomia candida(Collembola)[J].Ecotoxicology and EnvironmentalSafety,2006,63(2):216-225
• [6]Ma W C,Bodt J.Differences in toxicity of the insecticide chlor-pyrifos to six species of earthworms(Oligochaeta,Lunbricidae)in standardized soil tests[J].Bulletin of Environmental Contam-ination and Toxicology,1993,50(6):864-870
• [7]刘伟,朱鲁生,王军,等.毒死蜱、马拉硫磷和氰戊菊酯对赤子爱胜蚓(Eisenia foetida)的急性毒性[J].生态毒理学报,2009,4(4):597-601Liu W,Zhu L S,Wang J,et al.Acute toxicological dosages of chlorpyrifos,malathion and fenvalerate on earthworm Eisenia foe-tida[J].Asian Journal of Ecotoxicology,2009,4(4):597-601(in Chinese)
• [8]OECD.OECD Guideline for Testing of Chemicals,No.207:Earthworm,Acute Toxicity Tests[S].Paris:OECD,1984
• [9]孙敬芳.动物实验方法学[M].北京:人民卫生出版社,2001,362-363
• [10]张壬午,李治祥,白清云,等.化学农药对生态环境安全评价研究Ⅱ.化学农药对蚯蚓的毒性与评价[J].农村生态环境,1986,2(2):14-18Zhang R W,Li Z X,Bai Q Y,et al.Study on the eco-environ-mental evaluation of chemical pesticideⅡ.Toxicity and evalua-tion of chemical pesticides to earthworm[J].Rural Eco-Envi-ronment,1986,2(2):14-18(in Chinese)
• [11]Heimbach F.Correlations between three methods for determining the toxicity of chemicals to earthworms[J].Pesticide Science,1984,15(6):605-611
• [12]Kuperman R G,Checkai R T,Simini M,et al.Toxicity bench-marks for antimony,barium,and beryllium determined using re-production endpoints for Folsomia candida,Eisenia fetida,and Enchytraeus crypticus[J].Environmental Toxicology and Chem-istry,2006,25(3):754-762
• [13]Roberts B L,Drough H W.Relative toxicities of chemicals to the earthworm Eisenia foetide[J].Environmental Toxicology and Chemistry,1984,3(1):67-78
• [14]Davies N A,Hodson M E,Black S.The influence of time on lead toxicity and bioaccumulation determined by the OECD earth-worm toxicity test[J].Environmental Pollution,2003,121(1):55-61
• [15]Edwards C A,Bohnlen P.The effects of toxic chemicals on earthworms[J].Reviews of Environmental Contamination and Toxicology,1992,125:23-99
• [16]Callahan C A,Shirazi M A,Neuhauser E F.Comparative toxici-ty of chemicals to earthworms[J].Environmental Toxicology and Chemistry,1994,13(2):291-298
• [17]Dureja P,Patra D,Johnson S,et al.Effect of agrochemicals on earthworms[J].Toxicological and Environmental Chemistry,1999,71(3):397-404
• [18]Stenersen J.Action of pesticides on earthworms.Part I:The tox-icity cholinesterase-inhibiting insecticides to earthworms as evalu-ated by laboratory tests[J].Pesticide Science,1979,10(1):66-74
• [19]Sun J Y,Liang P,Gao X W.Inheritance of resistance to a new non-steroidal ecdysone agonist,fufenozide,in the diamondback moth,Plutella xylostella(Lepidoptera:Plutellidae)[J].Pest Management Science,2010,66(4):406-411
• [20]Wang Y H,Gao C F,Xu Z P,et al.Buprofezin susceptibility survey,resistance selection and preliminary determination of the resistance mechanism in Nilaparvata lugens(Homoptera:Del-phacidae)[J].Pest Management Science,2008,64(10):1050-1056
• [21]Gibbs M H,Wicker L F,Stewart A J.A method for assessing sublethal effects of contaminants in soils to the earthworm,Eise-nia foetida[J].Environmental Toxicology and Chemistry,1996,15(3):360-368