李娇;王姮;韩昭衡;石生宝;王永华;丁君;

• 1:大连海洋大学农业部海洋水产增养殖学与生物技术重点开放实验室

摘要(Abstract)：

为初步探讨农药对海胆胚胎的急性毒性效应,以虾夷马粪海胆(Strongylocentyotus internedius)为实验材料,研究了8种常见农药--草甘膦(Glyphosate)、百草枯(Paraquat)、异菌脲(Iprodione)、代森锰锌(Mancozeb)、三唑醇(Triadimenol)、霜霉威(Propamocarb)、百菌清(Chlorothalonil)和速克灵(Procymidone)对海胆胚胎各发育期(二细胞期、四细胞期、上浮囊胚期、原肠期、棱柱幼体期、四腕幼虫期)的半数效应浓度(EC50),并分析了正辛醇/水分配系数(LogP)与EC50的关系.结果显示:1)8种农药对海胆胚胎各发育期均具有一定的急性毒性,以四腕幼虫期为例,8种农药的EC50值分别为草甘膦(3.99mg·L-1)>百草枯(10.38mg·L-1)>代森锰锌(20.77mg·L-1)>速克灵(55.42mg·L-1)>霜霉威(56.09mg·L-1)>异菌脲(86.29mg·L-1)>三唑醇(273.20mg·L-1)>百菌清(647.38mg·L-1).2)LogP与EC50呈现一定的正相关性,即随着LogP的增加,各农药对海胆胚胎的抑制率逐渐降低.

关键词(KeyWords)： 农药;虾夷马粪海胆;胚胎发育;毒性影响

Abstract:

Keywords:

基金项目(Foundation): 国家高技术研究发展计划(863)项目(No.2006AA10A411);; 国家海洋局海洋溢油鉴别与损害评估技术重点实验室开放基金项目

作者(Author): 李娇;王姮;韩昭衡;石生宝;王永华;丁君;

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参考文献(References)：

• Beiras R,Bellas J,Fernández N,Lorenzo J I,Cobello-GarcíaA.2003.Assessment of coastal marine pollution in Galicia(NWIberian Peninsula):metal concentrations in seawater,sedimentand mussels(Mytilus galloprovincialis)versus embryo-larvalbioassays using Paracentrotus lividus and Ciona intestinalis[J].Marine Environmental Research,56(4):531-553
• Bellas J,Beiras R,Mario-Balsa J C,Fernández N.2005.Toxicity of organic compounds to marine invertebrate embryosand larvae:a comparison between the sea urchin embryogenesisbioassay and alternative test species[J].Ecotoxicology,14(3):337-353
• Bellas J,Beiras R,Vázquez E.2003.A standardisation of Cionaintestinalis(Chordata,Ascidiacea)embryo-larval bioassay forecotoxicological studies[J].Water Research,37(19):4613-4622
• Berdyshev E V,Vaskovsky V E,Vaschenko M A.1995.Seaurchins—a new model for PAF research in embryology[J].Comparative Biochemistry and Physiology,Part B,Biochemistry&Molecular Biology,110(3):629-632
• Carr R S,Chapman D C.1995.Comparison of methods forconducting marine and estuarine sediment porewater toxicitytests—Extraction,storage,and handling techniques[J].Archivesof Environmental Contamination and Toxicology,28(1):69-77
• Chang Y Q,Ding J,Song J.2004.Biological Study and Cultureof the Sea Cucumbers and Urchin[M].Beijing:Ocean Press(inChinese)
• Hamdoun A M,Griffin F J,Cherr G N.2002.Tolerance tobiodegraded crude in marine invertebrate embryos and larvae isassociated with expression of a multixen0biotic resistancetransporter[J].Aquatic Toxicology,61(1-2):127-140
• He Y B,Zhao Y H,Wang L S,An F C,Mo H H,Yang KW,Liu Y,Yu G,Xu X B.1994.Determination of n-octanol/water partition coefficient of organic chemicals[J].EnvironmentalChemistry,13(3):195-196(in Chinese)
• He Z W,Xi Y L,Chen Y,Zhang G,Wen X L.2009.Effectsof DDT on model ecosystems of ponds[J].Asian Journal ofEcotoxicology,4(4):552-560(in Chinese)
• His E,Beiras R,Seaman R N L.1999.The assessment ofmarine pollution-bioassay with bivalve embryos and larvae[J].Advances in Marine Biology,37:1-178
• Kobayashi N,Okamura H.2005.Effects of heavy metals on seaurchin embryo development.Part2.Interactive toxic effects ofheavy metals in synthetic mine effluents[J].Chemosphere,61(8):1198-1203
• Kubinyi H.1993.3D-QSAR in Drug Design:Theory,Methodsand Applications[M].Leiden:ESCOMScience Publishers,506-522
• Larrain A,Riveros A,Silva J,Bay—Schmith E.1999.Toxicityof metals and pesticides using the sperm cell bioassay with thesea urchin Arbacia spatuligera[J].Bulletin of EnvironmentalContamination and Toxicology,62(6):749-757
• Liu W P.2006.Environmental Chemistry of Pesticide[M].Beijing:Chemical Industry Press(in Chinese)
• Manzo S,Buono S,Cremisini C.2006.Toxic effects of irgaroland diuron on sea urchin Paracentrotus lividus early development,fertilization,and offspring quality[J].Archives of EnvironmentalContamination and Toxicology,51(1):61-68
• Marc J,Maguer C,BelléR,Mulner-Lorillon O.2002.Sharpdose-and time-dependent toxicity of mercuric cholride at thecellular level in sea urchin embryos[J].Archives of Toxicology,76(7):388-391
• Phillips B M,Nicely P A,Hunt J W,Anderson B S,TjeerdemaR S,Palmer S E,Palmer F H,Puckett H M.2003.Toxicity ofcadmium-copper-nickel-zinc mixtures to larval purple sea urchins(Strongylocentrotus purpuratus)[J].Bulletin of EnvironmentalContamination and Toxicology,70(3):592-599
• Richard A M,Hunter E S.1996.Quantitative structure-activityrelationships for the developmental toxicity of haloacetic acids inmammalian whole embryo culture[J].Teratology,53(6):352-360
• Ringwood A H.1991.Short-term accumulation of cadmium byembryos,larvae,and adults of a Hawaiian bivalve,Isognomoncalifornicum[J].Journal of Experimental Marine Biology andEcology,149(1):55-66
• Semenova M N,Kiselyov A,Semenov V V.2006.Sea urchinembryo as a model organism for the rapid functional screening oftubulin modulators[J].BioTechniques,40(6):765-774
• Sun X F,Ding J,Zhang H,Jia Y P,Wang Y H.2009.Toxiceffects of twelve pesticides on the development of sea urchinembryos[J].Asian Journal of Ecotoxicology,4(1):147-151(inChinese)
• Woodworth J G,King C,Miskiewicz A G,Laginestra E,SimonJ.1999.Assessment of the comparative toxicity of sewage effluentfrom10sewage treatment plants in the area of Sydney,Australiausing an amphipod and two sea urchin bioassays[J].MarinePollution Bulletin,39(1-12):174-178
• Wu Y H,Wu Y T,He L Y.2000.Application of n-octanol/water partition coefficient[J].Guangdong Pharmaceutical Journal,10(4):12-14(in Chinese)
• Yue W J,Wang Z H,Wang Q J,Liang W J,Li W J.2009.Toxic effects of cypermethrin on Chattonella marina[J].AsianJournal of Ecotoxicology,4(2):251-257(in Chinese)
• 常亚青,丁君,宋坚.2004.海参、海胆生物学研究与养殖[M].北京:海洋出版社
• 何艺兵,赵元慧,王连生,安凤春,莫汉宏,杨克武,刘晔,余刚,徐晓白.1994.有机化合物正辛醇/水分配系数的测定[J].环境化学,13(3):195-196
• 何忠文,席贻龙,陈艳,张根,温新利.2009.DDT对池塘模型生态系统的影响[J].生态毒理学报,4(4):552-560
• 刘维屏.2006.农药环境化学[M].北京:化学工业出版社
• 孙雪峰,丁君,张辉,贾艳平,王永华.2009.12种常见农药对海胆胚胎发育的毒性影响[J].生态毒理学报,4(1):147-151
• 吴义辉,伍玉甜,何丽瑜.2000.药物在辛醇-水体系分配系数的应用[J].广东药学,10(4):12-14
• 岳文洁,王朝晖,王桥军,梁文君,李维杰.2009.氯氰菊酯对海洋卡盾藻的毒性效应[J].生态毒理学报,4(2):251-257