张喆;王学锋;马胜伟;陈海刚;王涛;蔡文贵;贾晓平;

• 1:中国水产科学研究院南海水产研究所广东省渔业生态环境重点实验室
• 2:广东海洋大学水产学院
• 3:青岛中油华东院安全环保有限公司

摘要(Abstract)：

十溴联苯醚(BDE-209)是目前唯一仍在全球范围内广泛使用的多溴联苯醚阻燃剂,其环境行为及生物效应逐渐成为环境污染领域研究的热点。选用2(LD组)、10(MLD组)、50(MHD组)及250μg.L-1(HD组)4个浓度的BDE-209作用于紫红笛鲷,探讨BDE-209对紫红笛鲷鳃抗氧化酶(SOD、CAT及POD)活性和MDA含量的影响。结果显示:BDE-209可以显著促进SOD和CAT活性,且随着暴露时间延长CAT活性呈现逐渐增加的趋势。暴露7d后,MLD组SOD活性达到最高68.31U.mg-1,是对照组(CK)的5.50倍,HD组CAT活性达到6.31U.mL-1,其次为MHD组的5.96U.mL-1。BDE-209对鳃POD活性呈现先促进后抑制的作用,暴露3d时各浓度POD活性均显著高于CK组(P<0.05);7d时各浓度POD活性则受到显著抑制(P<0.05),清水恢复15d后各浓度组与CK组没有显著差异(P>0.05)。暴露期间各浓度组MDA含量均显著高于CK组(P<,并且随暴露时间延长呈现先增加后减少的趋势,7d后HD组MDA含量达到最高,为6.99nmol.mg-1,清水恢复15d后,LD和MLD组MDA含量与CK组没有显著性差异(P>0.05)。以上结果表明:BDE-209可以诱导紫红笛鲷产生氧化应激效应,CAT和SOD则在氧自由基清除中发挥作用。

关键词(KeyWords)： 持久性有机物;十溴联苯醚;紫红笛鲷;抗氧化酶

Abstract:

Keywords:

基金项目(Foundation): 广东省自然基金项目(S2011040005251);; 广东省科技计划项目(2009B030600001);; 中央级公益性科研院所基本科研业务费专项资金项目(2012TS14,2012A0203)

作者(Author): 张喆;王学锋;马胜伟;陈海刚;王涛;蔡文贵;贾晓平;

Email:

DOI:

参考文献(References)：

• [1]Kierkegaard A,Sellstrm U,McLachlan S M.Environ-mental analysis of higher brominated diphenyl ethers and decabromodiphenyl ethane[J].Journal of Chromatogra-phy A,2009,1216(3):364-375
• [2]李欣年,黄敏,虞太六.十溴联苯醚(BDE-209)对成年大鼠甲状腺激素的影响[J].生态毒理学报,2009,4(4):500-506Li X N,Huang M,Yu T L.Disturbance of decabrominat-ed diphenyl ether(BDE-209)to thyroid hormones of a-dult rats in vivo[J].Asian Journal of Ecotoxicology,2009,4(4):500-506(in Chinese)
• [3]陈社军,麦碧娴,曾永平,等.珠江三角洲及南海北部海域表层沉积物中多溴联苯醚的分布特征[J].环境科学学报,2005,25(9):1265-1271Chen S J,Mai B X,Zeng Y P,et al.Polybrominated di-phenyl ethers(PBDEs)in surficial sediments of the Pearl River Delta and adjacent South China Sea[J].Acta Scien-tiae Circumatantiae,2005,25(9):1265-1271(in Chi-nese)
• [4]Wang Z,Ma X D,Lin Z S,et al.Congener specific dis-tributions of polybrominated diphenyl ethers(PBDEs)in sediment and mussel(Mytilus edulis)of the Bo Sea,Chi-na[J].Chemosphere,2009,74(7):896-901
• [5]Mai B,Chen S,Luo X,et al.Distribution of polybromi-nated diphenyl ethers in sediments of the Pearl River Delta and adjacent South China Sea[J].Environmental Science&Technology,2005,39(10):3521-3527
• [6]向彩红,罗孝俊,余梅,等.珠江河口水生生物中多溴联苯醚的分布[J].环境科学,2006,27(9):1732-1737Xiang C H,Luo X J,Yu M,et al.Distribution of poly-brominated diphenyl ethers in aquatic species from the Pearl River Estuary[J].Environmental Science,2006,27(9):1732-1737(in Chinese)
• [7]孟范平,李卓娜.多溴联苯醚(PBDEs)在海洋环境中的行为研究进展[J].中国海洋大学学报,2009,39(2):285-289Meng F P,Li Z N.Research progress on the behavior of polybrominated diphenyl ethers(PBDEs)in marine envi-ronment[J].Periodical of Ocean University of China,2009,39(2):285-289(in Chinese)
• [8]周科,马陶武,朱程,等.2,2’,4,4’-四溴联苯醚(BDE-47)污染沉积物对铜锈环棱螺肝胰脏的SOD、CAT和EROD活性的影响[J].环境科学学报,2010,30(8):1666-1673Zhou K,Ma T W,Zhu C,et al.Effects of2,2',4,4'-tetra-bromodiphenyl ether(BDE-47)-contaminated sediments on SOD,CAT,and EROD activities in the hepatopan-creas of Bellamya aeruginosa[J].Acta Scientiae Circum-stantiae,2010,30(8):1666-1673(in Chinese)
• [9]唐学玺,柯可,王悠.多溴联苯醚在海洋环境中的分布及其对海洋生物的影响研究[J].海洋湖沼通报,2010,1(1):36-46Tang X X,Ke K,Wang Y.The distribution of polybrom-inated diphenyl ethers(PBDEs)and its effects on marine organisms in marine environment[J].Transactions of O-ceanology and Limnology,2010,1(1):36-46(in Chi-nese)
• [10]瞿建宏,陈家长,孟顺龙,等.鲫鱼肝脏微粒体CYP450同工酶对多溴联苯醚胁迫的应答[J].农业环境科学学报,2009,28(10):2023-2028Qu J H,Chen J Z,Meng S L,et al.Responses of CYP450isoenzyme in liver microsome of Carassius aura-tus Linn.under polybrominated diphenyl ethers stress[J].Journal of Agr-oEnvironment Science,2009,28(10):2023-2028(in Chinese)
• [11]蔡泽平,李沫,胡超群.紫红笛鲷繁殖特性及诱导产卵的初步研究[J].热带海洋学报,2002,21(4):22-29Cai Z P,Li M,Hu C Q.A preliminary study on repro-ductive characteristics and induced spawning of mangrove red snapper Lutjanus argentimaculatus[J].Journal of Tropical Oceanography,2002,21(4):22-29(in Chi-nese)
• [12]Shao J,Eckert M L,Lee L E J,et al.Comparative oxygen radical formation and toxicity of BDE-47in rainbow trout cell lines[J].Marine Environmental Research,2008,66(1):7-8
• [13]Giordano G,Kavanagh T J,Costa L G.Neurotoxicity of a polybrominated diphenyl ether mixture(DE-71)in mouse neurons and astrocytes is modulated by intracellu-lar gluthatione levels[J].Toxicology Applied Pharmacol-ogy,2008,232(2):161-168
• [14]Chen J S,Chun L F,Sun W W,et al.Assessment of the neurotoxic mechanisms of decabrominated diphenyl ether(PBDE-209)in primary cultured neonatal rat hippocam-pal neurons includes alterations in second messenger sig-naling and oxidative stress[J].Toxicology Letters,2010,192(3):431-439
• [15]Bellés M,Alonso V,Linares V,et al.Behavioral effects and oxidative status in brain regions of adult rats exposed to BDE-99[J].Toxicology Letters,2010,194(1-2):1-7
• [16]Costa G L,Giordano G.Is decabromodiphenyl ether(BDE-209)a developmental neurotoxicant?[J].Neuro-toxicology,2011,32(1):9-24
• [17]Tseng L H,Li M H,Tsai S S,et al.Development expo-sure to decabromodiphenyl ether(PBDE209):Effects on thyroid hormone and hepatic enzyme activity in malemouse offspring[J].Chemosphere,2008,70(4):640-647
• [18]Zhang C F,Liu F C,Liu X B,et al.Protective effect of N-acetylcysteine against BDE-209-induced neurotoxicity in primary cultured neonatal rat hippocampal neurons in vitro[J].International Journal of Developmental Neuro-science,2010,28(6):521-528
• [19]蔡云梅,张文兵,盛国英,等.十溴联苯醚在单次暴露的孕和非孕SD大鼠血液内的吸收分布[J].生态环境学报,2010,19(5):1009-1013Cai Y M,Zhang W B,Sheng G Y,et al.Absorption and distribution of BDE-209in the blood of pregnant and non-pregnant Sprague-Dawley rats exposed by single dose of decabromodiphenyl ether[J].Ecology and Envi-ronmental Sciences,2010,19(5):1009-1013(in Chi-nese)
• [20]杜红燕,朱琳,陈中智,等.十溴联苯醚的毒理学效应研究进展[J].毒理学杂志,2008,22(1):50-52
• [21]唐学玺,张培玉.蒽对黑鲪超氧化物歧化酶活性的影响[J].水产学报,2000,24(3):217-220Tang X X,Zhang P Y.Effects of anthracene on activity of superoxide dismutase in Sebastodes fuscescens[J].Journal of Fisheries of China,2000,24(3):217-220(in Chinese)
• [22]Alonso V,Linares V,Bellés M,et al.Effects of BDE-99on hormone homeostasis and biochemical parameters in adult male rats[J].Food and Chemical Toxicology,2010,48(8-9):2206-2211
• [23]杜红燕,朱琳,张清敏,等.十溴联苯醚对底栖生物和土壤微生物的毒理效应探讨[J].农业环境科学学报,2008,27(2):502-507Du H Y,Zhu L,Zhang Q M,et al.Toxicological effects of decabromodiphenyl ether exposure on benthonic inver-tebrates and soil microorganisms[J].Journal of Agro-En-vironment Science,2008,27(2):502-507(in Chinese)
• [24]聂凤琴,瞿建宏,杨光,等.离体条件下十溴联苯醚暴露对鲫鱼肝脏线粒体的氧化损伤[J].安全与环境学报,2008,8(5):5-8Nie F Q,Qu J H,Yang G,et al.Oxidative damage of de-cabromodiphenyl ether to the mitochondria from the liver of Carassius auratus in vitro[J].Journal of Safety and Environment,2008,8(5):5-8(in Chinese)
• [25]吴伟,聂凤琴,瞿建宏.多溴联苯醚对鲫鱼离体肝脏组织中CAT和GSH-Px的影响[J].生态环境学报,2009,18(2):408-413Wu W,Nie F Q,Qu J H.The in vitro effects of tetrabro-modiphenyl ether and decabromodiphenyl ether on the activities of catalase and glutathione peroxidase in the liv-er of Carassius auratus[J].Ecology and Environmental Sciences,2009,18(2):408-413(in Chinese)
• [26]范灿鹏,王奇,刘昕宇,等.四溴联苯醚对剑尾鱼毒性及其抗氧化系统的影响[J].环境科学学报,2011,31(3):642-648Fan C P,Wang Q,Liu X Y,et al.Toxicity and effect of tetrabromodiphenyl ether upon the antioxidant defense system of swordtail fish(Xiphophorus helleri)[J].Acta Scientiae Circumstantiae,2011,31(3):642-648(in Chi-nese)
• [27]丁磊,吴萍,蔡春芳,等.Cd对鲫血清溶菌酶和过氧化物酶的影响[J].农业科学环境学报,2004,23(2):243-245Ding L,Wu P,Cai C F,et al.Effects of CdCl2on lyso-zyme and peroxidase activities of Carassius auratus[J].Journal of Agr-oEnvironment Science,2004,23(2):243-245(in Chinese)
• [28]刘香江.重金属铜和镉(Cu和Cd)及乙酰甲胺磷对矛尾复虾虎鱼毒性效应的研究[D].武汉:华中农业大学,2010:26-37Liu X J.Toxic effects of waterborne heavy metal(copper and cadmium)and acephate exposure on Synechogobius hasta[D].Wuhan:Huazhong Agricultural University,2010:26-37(in Chinese)
• [29]陈海刚,马胜伟,林钦,等.氯化三丁基锡对黑鲷鳃和肝组织SOD、MDA和GPx的影响[J].南方水产,2009,5(2):23-27Chen H G,Ma S W,Lin Q,et al.Effects of tributyltin chloride(TBTCl)on SOD activities,MDA contents and GPx activities in gill and liver of the black porgy(Sparus macrocephalus)[J].South China Fisheries Science,2009,5(2):23-27(in Chinese)
• [30]张景飞,王晓蓉,沈骅.低浓度2,4-DCP对鲫鱼肝脏抗氧化防御系统的影响[J].中国环境科学,2003,23(5):531-534Zhang J F,Wang X R,Shen H.Influence of low-level2,4-DCP on the antioxidant defense system in livers of Car-assius auratus[J].China Environmental Science,2003,23(5):531-534(in Chinese)