孟紫强;吕亚峰;

• 1:山西大学环境科学与工程研究中心
• 2:山西大学环境医学与毒理学研究所 太原030006

摘要(Abstract)：

为了探讨体内亚硫酸氧化酶的分布特点及随年龄的变化规律,以Wistar大鼠为实验材料,研究了不同月龄大鼠脏器中的亚硫酸氧化酶活性.以细胞色素C为电子受体,应用分光光度法测定了不同月龄(1、4、10个月)大鼠肝、肾、肺、脾、脑、胃及胸主动脉中亚硫酸氧化酶的活性.结果表明:1)亚硫酸氧化酶在大鼠全身主要脏器中普遍存在,且在不同脏器中该酶的活性不同,从强到弱的顺序基本表现为:肝、肾>胃>脑>胸主动脉血管>肺>脾;2)肝、肾、血管组织中亚硫酸氧化酶活性与大鼠年龄相关,肝脏亚硫酸氧化酶活性随年龄的增加而增加(Spearman,r=0.674,p<0.001),而肾脏和胸主动脉血管该酶活性随年龄的增加而减小(Spearman,r=-0.756,p<0.001;r=-0.629;p<0.05),其余脏器未见明显变化;3)亚硫酸氧化酶活性降低可能是一种机体衰老的生化标志物.

关键词(KeyWords)： 亚硫酸氧化酶;脏器;年龄;大鼠;衰老

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(No.20677035;No.20477023);; 山西省自然科学基金项目(No.20031092)

作者(Author): 孟紫强;吕亚峰;

Email:

参考文献(References)：

• 通讯作者简介:孟紫强(1939—),男,山西临汾人,教授,博士生导师,研究方向:环境医学与毒理学.从事
• Bai J Y,Meng Z Q.2003.Effect of sulfur dioxide on therelation of lienic apoptasis and glutathione content[J].Journalof Health Toxicology,17(1):42-44(in Chinese)
• Bokov A,Chaudhuri A,Richardson A.2004.The role ofoxidative damage and stress in aging[J].Mechanisms of Ageingand Development,125(10-11):811-826
• Komarnisky L A,Christopherson R J,Basu T K.2003.Sulfur:its clinical and toxicologic aspects[J].Nutrition,19(1):54-61
• Meng Z Q.2003.Oxidative damage of sulfur dioxide on variousorgans of mice:sulfur dioxide systemic oxidative damage agent[J].Inhalation Toxicology,l5(2):l8l-l95
• Meng Z Q.2003.Qxidation stress effects of SO2and itssystemic toxin[J].China Public Health,19(12):1422-1424(in Chinese)
• Meng Z Q,Bai J L.2003.Damage effects of sulfur dioxideinhalation on DNA damages in alveolar macrophages from malerats[J].Acta Scientiae Circumstantiae,23(6):825-827(inChinese)
• Meng Z Q,Bai J L,Qin G H.2005a.The effect of sulfurdioxide on gene expression of hepatic and pulmonary threeapoptosis-related genes and four cytochrome P450gene[J].Journal of Toxicology,19(A03):229(in Chinese)
• Meng Z Q,Li R J,Bai J L,Zhang Q X,Li Y.2005b.Damage effects of sulfite sodium on DNA in cells from micevarious organs[J].Food Science,26(10):203-206(inChinese)
• Meng Z Q,Nie A F,Sang N,Du Z Q,Wei C L.2006a.Effects of SO2derivatives on ion channels in acutely isolated ratneurons and cardiomyocytes[J].Asian Journal of Ecotoxicology,1(2):123-134(in Chinese)
• Meng Z Q,Qin G H.2006b.Alterations of gene expressionprofiles in lungs of rats following sulfur dioxide inhalation[J].Chin J Public Health,22(3):274-276(in Chinese)
• Meng Z Q,Qin G H,Zhang B.2005.DNA damage in micetreated with sulfur dioxide by inhalation[J].Environmental andMolecular Mutagenesis,46(3):150-155
• Meng Z Q,Qin G H,Zhang B,Ruan A D,Zhang J B.2002a.Damage effects of sulfur dioxide inhalation on DNA oflung cells from mice[J].Acta Scientiae Circumstantiae,22(3):389-392(in Chinese)
• Meng Z Q,Ruan A D,Zhang B,Sang N,Zhang J B.2002b.Micronuclei induced by sulfur dioxide inhalation in bone marrowcells of mice[J].J Environ Health,19(4):298-299(in Chinese)
• Meng Z Q,Sang N,Zhang B,Zhang J B.2001a.Inductiveeffects of intravital derivatives of sulfur dioxide on frequenciesof micronucleus of lung fibroblast cells in Chinese hamster[J].J Environ Health,18(1):7-10(in Chinese)
• Meng Z Q,Wang S D.2007.SO2——A secret biologicalsmall molecule:The biphasic roles of SO2and its derivatives onthe blood vessel tension[J].Asian Journal of Ecotoxicology,2(2):158-163(in Chinese)
• Meng Z Q,Zhang B.2002c.Induction effects of sulfur dioxideinhalation on chromosomal aberrations in mouse bone marrowcells[J].Chin J Prev Med,36(4):229-231(in Chinese)
• Meng Z Q,Zhang B,Qin G H.2001b.Oxidation damage ofsulfur dioxide on various organs of mice[J].Acta ScientiaeCircumstantiae,21(6):769-773(in Chinese)
• Meng Z Q,Zhang L Z.1994.Chromosomal aberrations,sisterchromatid exchanges and micronuclei induced in humanlymphocytes by sodium bisulfite(sulfur dioxide)[J].ActaGenetica Sinica,21(1):1-6(in Chinese)
• Mudd S H,Irreverre F,Laster L.1967.Sulfite oxidasedeficiency in man:demonstration of the enzymatic defect[J].Science,156(3782):1599-1602
• Rupar C A,Gillett J,Gordon B A,Ramsay D A,Johnson JL,Garrett R M,Rajagopalan K V,Jung J H,Bacheyie G S,Sellers A R.1996.Isolated sulfite oxidase deficiency[J].Neuropediatrics,27(6):299-304
• Woo W H,Yang H Y,Wong K P,Halliwell B.2003.Sulphite oxidase gene expression in human brain and in otherhuman and rat tissues[J].Biochemical and Biophysical ResearchCommunications,305(3):619-623
• Xie J F,Meng Z Q.2006a.Oxidative damage of SO2derivatives on the mice spleen and stomach tissues protein[J].China Environmental Science,26(5):595-598(in Chinese)
• Xie J F,Meng Z Q.2006b.Oxidative damage of sulfur dioxidederivatives on hearts,livers and lungs of mice[J].Acta ScientiaeCircumstantiae,26(7):1175-1179(in Chinese)
• 白剑英,孟紫强.2003.二氧化硫诱导小鼠脾细胞凋亡与谷胱甘肽水平的关系[J].卫生毒理学杂志,17(1):42-44
• 孟紫强.2003.氧化应激效应与二氧化硫全身性毒作用研究[J].中国公共卫生,19(12):1422-1424
• 孟紫强,白巨利.2003.二氧化硫对大鼠肺泡巨噬细胞DNA的损伤作用[J].环境科学学报,23(6):825-827
• 孟紫强,白巨利,秦国华.2005a.SO2对大鼠肝、肺3种细胞凋亡相关基因和细胞色素P4504种基因表达的影响[J].毒理学杂志,19(A03):229
• 孟紫强,李瑞金,白巨利,张全喜,李屹.2005b.亚硫酸钠对小鼠不同脏器的DNA损伤[J].食品科学,26(10):203-206
• 孟紫强,聂爱芳,桑楠,杜正清,魏彩玲.2006a.SO2衍生物对大鼠神经元和心肌细胞几种离子通道的影响[J].生态毒理学报,1(2):123-134
• 孟紫强,秦国华.2006b.二氧化硫吸入致大鼠肺组织基因表达谱改变[J].中国公共卫生,22(3):274-276
• 孟紫强,秦国华,张波,阮爱东,张建彪.2002a.二氧化硫对小鼠肺细胞DNA损伤的研究[J].环境科学学报,22(3):389-392
• 孟紫强,阮爱东,张波,桑楠,张建彪.2002b.SO2吸入对小鼠骨髓细胞微核的诱发效应[J].环境与健康杂志,19(4):298-299
• 孟紫强,桑楠,张波,张建彪.2001a.二氧化硫体内衍生物诱发中国仓鼠肺细胞微核的效应[J].环境与健康杂志,18(1):7-10
• 孟紫强,王少东.2007.SO2——一种神秘的生物小分子:SO2及其衍生物对血管张力的双相-双向性调节作用[J].生态毒理学报,2(2):158-163
• 孟紫强,张波.2002c.二氧化硫吸入诱发小鼠骨髓细胞染色体畸变的效应[J].中华预防医学杂志,36(4):229-231
• 孟紫强,张波,秦国华.2001b.二氧化硫对小鼠不同组织器官的氧化损伤作用[J].环境科学学报,21(6):769-773
• 孟紫强,张连珍.1994.亚硫酸氢钠(SO2)对人血淋巴细胞染色体畸变、姊妹染色单体互换及微核的效应[J].遗传学报,21(1):1-6
• 解静芳,孟紫强.2006a.SO2衍生物对小鼠脾、胃组织蛋白质的氧化损伤[J].中国环境科学,26(5):595-598
• 解静芳,孟紫强.2006b.二氧化硫衍生物对小鼠心、肝、肺蛋白质的氧化损伤作用[J].环境科学学报,26(7):1175-1179