12种常见农药对海胆胚胎发育的毒性影响Toxic Effects of Twelve Pesticides on the Development of Sea Urchin Embryos
孙雪峰;丁君;张辉;贾艳平;王永华;
摘要(Abstract)：
为了研究低浓度下农药对海胆胚胎的毒性影响,以及正辛醇/水分配系数(LogP)与致死率的关系,实验研究了12种常见农药(阿维菌素、硫丹、除虫脲、甲氰菊酯、三环唑、使它隆、抑霉唑、戊唑醇、磺胺吡啶、磺胺甲噁唑、磺胺二甲嘧啶和磺胺嘧啶)在低浓度(0.01mg.L-1)下对光棘球海胆(Strongylocentrotus nudus)胚胎发育的急性毒性影响.结果显示:1)实验浓度下,磺胺吡啶、三环唑、磺胺嘧啶和抑霉唑分别对受精膜举起期、2细胞期、上浮囊胚期和棱柱幼体期相对致死率最高,分别达到3.70%、6.19%、5.84%和6.07%.2)农药对海胆胚胎发育存在低剂量有毒物质的刺激作用(Hormesis现象).3)在海胆胚胎各发育期,LogP与相对致死率呈现一定的负相关性,即随着LogP的增加,各农药对海胆胚胎的相对致死率逐渐下降.
关键词(KeyWords)： 农药;光棘球海胆;胚胎发育;毒性影响
基金项目(Foundation): 农业部渔业生态环境重点开放实验室开放基金;; 国家863重大项目课题(No.2006AA10A411);; 辽宁农业攻关项目(No.2007203004)
作者(Author): 孙雪峰;丁君;张辉;贾艳平;王永华;
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参考文献(References)：
- Bellas J.2006.Comparative toxicity of alternative antifoulingbiocides on embryos and larvae of marine invertebrates[J].Science of the Total Environment,367:573-585
- Berdyshev E V,Vaskovsky V E,Vaschenko M A.1995.Seaurchins—a new model for PAF research in embryology[J].Comparative Biochemistry and Physiology,Part B,Biochemistry&Molecular Biology,110:629-632
- Calabrese E J.2002.Hormesis:changing view of the dose-response,a personal account of the history and current status[J].Mutation Research,511:181-189
- Chang Y Q,Ding J,Song J.2004.Biological Study and Cultureof the sea cucumbers and urchin[M].Beijing:Ocean Press(inChinese)
- Hansch C,Leo A.1979.Substitution Constants for CorrelationAnalysis in Chemistry and Biology[M].New York:WileyInterscience
- Kiyomoto M,Kikuchi A,Unuma T,Yokota Y.2006.Effects ofethynylestradiol and bisphenol on the development of sea urchinembryos and juveniles[J].Marine Biology,149:57-63
- Kobayashi N,Okamura H.2002.Effects of new antifouling compounds on the development on sea urchin[J].Marine PollutionBulletin,44:748-751
- Li H T,Qin Z F,Qin X F,Xia X J,Xu X B,Ma B H.Effects of the herbicide simazine on the survival and gonadaldevelopment of African clawed frogs(Xenopus laevis)[J].AsianJournal of Ecotoxicology,3:280-285(in Chinese)
- Liu W P.2006.Environmental Chemistry of Pesticide[M].Beijing:Chemical Industry Press(in Chinese)
- Manzo S,Buono S,Cremisini C.2006.Toxic effects of irgaroland diuron on sea urchin Paracentrotus lividus early development,fertilization,and offspring quality[J].Archives of EnvironmentalContamination and Toxicology,51:61-68
- Mwatibo J M,Green J D.1997.Effects of methoxychlor pre-exposure on sea urchin gametes[J].Bulletin of EnvironmentalContamination and Toxicology,58:589-595
- Richard A M,Hunter E S.1996.Quantitative structure-activityrelationships for the developmental toxicity of haloacetic acids inmammalian whole embryo culture[J].Teratology,53:352-360
- Semenova M N,Kiselyov A,Semenov V V.2006.Sea urchinembryo as a model organism for the rapid functional screening oftubulin modulators[J].BioTechniques,40:765-774
- Tao G H.2007.Research status of the mechanism of hormesis[J].Journal of the Graduates Sun Yat-sen University(NaturalSciences and Medicine),28:16-21(in Chinese)
- 常亚青,丁君,宋坚.2004.海参、海胆生物学研究与养殖[M].北京:海洋出版社
- 李焕婷,秦占芬,秦晓飞,夏晞娟,徐晓白,马保华.2008.除草剂西玛津对非洲爪蟾生存和性腺发育的影响[J].生态毒理学报,3:280-285
- 刘维屏.2006.农药环境化学[M].北京:化学工业出版社
- 陶功华.2007.低剂量兴奋效应作用机制的研究进展[J].中山大学研究生学刊(自然科学与医学版),28:16-21