吡虫啉对蜜蜂幼虫的室内毒性研究Indoor Toxicity Research of Imidacloprid on Honeybee Larvae
谭丽超;卜元卿;程燕;周军英;单正军;
摘要(Abstract)：
吡虫啉是目前使用最广泛的新烟碱类农药,其不合理使用破坏蜜蜂的嗅觉记忆能力,影响采集行为,导致多种亚致死效应。利用室内人工饲养蜜蜂幼虫技术,选取意大利蜜蜂幼虫为研究对象,经3 d适应性饲养后,连续3 d喂食含不同浓度的吡虫啉日粮,测定蜜蜂幼虫的存活率、化蛹率和羽化率,评估吡虫啉对蜜蜂幼虫生长发育影响。结果表明,吡虫啉处理剂量为15ng·幼虫~(-1)时,并不影响蜜蜂幼虫存活率、化蛹率和羽化率;当处理剂量达到150 ng·幼虫~(-1)时,蜜蜂幼虫羽化率下降,但不影响蜜蜂幼虫的存活率和化蛹率;幼虫7 d半数致死剂量为2 300 ng·幼虫~(-1)。高剂量吡虫啉对蜜蜂幼虫存活率、化蛹率和羽化率均存在影响,处理剂量越高,存活率、化蛹率和羽化率越低,呈高度负相关(r=-0.99,-0.94,-0.89),同时蜜蜂幼虫的羽化时间延长。研究可为吡虫啉的合理使用提供科学参考。
关键词(KeyWords)： 吡虫啉;蜜蜂幼虫;室内毒性
基金项目(Foundation): 2015年中央级公益性科研院所基本科研业务专项(农药对蜜蜂慢性毒性、幼虫毒性试验方法及配套技术研究)
作者(Author): 谭丽超;卜元卿;程燕;周军英;单正军;
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