涕灭威及其有毒代谢产物对DNA潜在损伤研究The Research of Aldicarb and It's Toxic Metabolites on DNA Potential Harm
宋艳;朱鲁生;王军;谢慧;刘伟;王秀国;王倩;钱博;
摘要(Abstract):
采用生物遗传监测系统,研究了涕灭威及其两个有毒代谢产物(涕灭威亚砜,涕灭威砜)对蚕豆及大蒜根尖细胞微核率的影响,以及对小牛胸腺DNA的加合作用.结果表明,两种植物根尖细胞微核试验测得的高浓度剂量诱导的微核率与对照组相比有显著性差异(p<0.05),说明涕灭威、涕灭威亚砜及涕灭威砜可能对蚕豆及大蒜根尖细胞有致突变性.并且配对资料差异的显著性检验表明,蚕豆根尖微核试验的敏感性明显高于大蒜根尖微核试验的敏感性.涕灭威及其两个有毒代谢产物与小牛胸腺DNA结合引起吸收光谱波峰的移位,而且存在着剂量关系,说明涕灭威、涕灭威亚砜及涕灭威砜可能对小牛胸腺DNA造成加合作用.
关键词(KeyWords): 涕灭威;涕灭威亚砜;涕灭威砜;微核试验;DNA加合
基金项目(Foundation): 国家自然科学基金(No.20477022);; 山东省优秀中青年科学家科研奖励基金(No.03BS123)
作者(Author): 宋艳;朱鲁生;王军;谢慧;刘伟;王秀国;王倩;钱博;
Email:
DOI:
参考文献(References):
- Beach A C, Gupta R C. 1992. Human biomonitoring and the 32P-postlabeling assay[J]. Carcinogenesis, 13: 1053
- Chen X Q. 2000. The genotoxicity study of water sample from water supply and drainage system using micronucleus in Vicia faba root tip cells [J]. Carcinogenesis Teratogenesis and Mutagenesis, 12(4): 214-218 (in Chinese)
- Chen Y G, Zhang Y. 2002. DNA-targeting bioassay guided isolation of active principles from natural medicinal plants [J] . The Chinese Journal of Modern Applied Pharmacy, 19 (1):1-3 (in Chinese)
- Cobb G P, Harper F D, Weisskopf C P. 2001. Nonlethal method for forensic evaluation of adducer exposure [J]. Environ Contain Toxicol, 40:77-88
- David W, Gaylor. 1992. Application of biomarkers to risk Assessment [J]. Environmental Health Perspectives, 98: 139 Degrassi F, Rizzoni M. 1982. Micronucleus test in Vicia faba root tips to detect mutagen damage in fresh-water pollution [J] . Mutat Res,97: 19
- Han G Y, Yang P. 2002. Synthesis and characterization of waterinsoluble and water-soluble dibutyltin (Ⅳ) porphinate complexes based on the iris (pyridinyl) porphyrin moiety, their anti-tumor activity in vitro and interaction with DNA [J]. J Inorg Biochem, 91 (1): 230-236
- Hou J L, Zhang Q.1988. Detection of water pollution with root-tip micronucleus technique in Allium sativum[J]. The Journal of Ecology, 7(3): 50-52 (in Chinese)
- Hulka B S, Wilcosk T C, Griffith J D. 1990. Biological markers in epidemiology [M]. New York: Oxford Univer Press
- Kumar C V, Emma H, Asuncion. 1993. DNA binding studies and site selective fluorescence sensitization of an anthryl probe [J].J Am Chem Soc,115: 8547-8553
- Li Y, Zhang Q M, Dai S G. 2003. Impact of aldicard and its complex pollution on DNA of zebrafish embryo [J]. Chinese Journal of Applied Ecology, 14(6): 982-984 (in Chinese)
- Lightfoot E N, Thome P S, Jones R L.1987. Laboratory studies on mechanisms for the degradation of Aldicarb, Aldicarb sulfoxide and Aldicarb sulfone[J]. Environ Toxicol Chem, 6: 377-394
- Rappaport S M, Ting D, Jin Z L et al. 1993. Application of Raney Nickel to measure adducts of Styrene Oxide with Hemoglobin and Alumin [J]. Chem Res Toxicol, 6: 238
- State Environmental Protection Agency of China. 2002. Detection of analytical methods to water and waste water [M]. 4rd Edition. Beijing: China Environmental Science Press (in Chinese)
- Te-Hsiu Ma. 198 2. Vicia Cytogenetic tests for environmental mutagens [J]. Mutation Research, 99: 257-271
- Ward J B.1996. Identification of needs in biomarker research [J]. Environ Health Perspect, 104(suppl 5): 895
- Ye Y, Hu J M, Zeng Y E. 2000. Comparison of different spectroscopic methods for the interaction of antitumour compounds with deoxyribonucleic acid [J] . Chinese Journal of Analytical Chemistry, 28(7): 798-804 (in Chinese)
- Zhang Q M, Xiong Y, Li D X, Dai S G. 2001. A simple and rapid procedure for detection of DNA damage caused by Aldicard [J]. Environmental Science, 22(5): 126-128 (in Chinese)
- 陈小倩.2000.应用蚕豆根尖微核技术检测上海给排水系统 中的致突变情况[J].致癌·致畸·致突变,12(4):214-218
- 陈业高,张燕.2002.以DNA为作用靶追踪分离天然植物药 活性成分[J].中国现代应用药学杂志,19(1):1-3
- 国家环境保护总局.2002.水和废水监测分析方法[M].第4 版.北京:中国环境科学出版社
- 候家龙,等.1988.应用大蒜根尖微核技术监测水质污染的初 步研究[J].生态学杂志,7(3):50-52
- 李阳,张清敏,戴树桂.2003.涕灭威及其复合污染对斑马鱼胚胎 DNA的影响[J].应用生态学报,14(6):982-984
- 叶勇,胡继明,曾云鹗.2000.抗癌金属络合物与脱氧核糖核酸作 用的谱学研究比较[J].分析化学,28(7):798-804
- 张清敏,熊瑛,李得翔,戴树桂.2001.一种简便快速检测涕灭威对 DNA损伤的方法[J].环境科学,22(5):126-128