全氟化合物对水生植物的生态效应研究Ⅱ——金鱼藻对水中PFOS的生物富集及生理响应Ecological Effects on Aquatic Plants Induced by Perfluorinated Compounds Ⅱ—Bioaccumulation and Physiological Response of Ceratophyllum Demersum in PFOS Polluted Water
孔潇潇;王铁宇;张晓军;蒋曌泽;周云桥;李奇锋;孟晶;朱朝云;
摘要(Abstract):
为探究全氟化合物对水生植物的生态效应,选择金鱼藻(Ceratophyllum demersum L.)为受试生物,设定5、100、1 000、10 000、50 000、100 000μg·L-1以及对照共7个PFOS浓度梯度进行水培,分析包括超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)在内的抗氧化系统酶和细胞色素含量等生理响应特征,并研究金鱼藻对PFOS的生物有效性及富集能力。结果表明:金鱼藻对PFOS的富集量最高可达3 180 mg·kg-1dw,最大富集系数高达40.7倍,可作为PFOS污染水体植物修复的遴选物种。随PFOS浓度升高,SOD活力总体上没有明显变化,但POD活力表现为双重效用,即低浓度促进酶活力积累,而高浓度抑制酶活力积累。CAT活力在低浓度组(0、5、100、1 000μg·L-1),未表现出明显异常,在高浓度组(10 000、50 000、100 000μg·L-1),出现先升高后降低的趋势。色素含量也反应较为敏感,低浓度处理后色素含量升高,高浓度处理则导致色素含量先升高后降低,最高浓度处理下一直处于较低水平,可作为评价PFOS污染水体的生理敏感性指标。
关键词(KeyWords): 全氟化合物;金鱼藻;PFOS生物有效性;抗氧化系统;生态效应
基金项目(Foundation): 国家自然科学基金项目(41171394);; 科技基础性工作专项(2013FY11110);; 中国科学院重点部署项目(KZZD-EW-TZ-12)
作者(Author): 孔潇潇;王铁宇;张晓军;蒋曌泽;周云桥;李奇锋;孟晶;朱朝云;
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参考文献(References):
- [1]Giesy J P,Kannan K.Global distribution of perfluorooctane sulfonate in wildlife[J].Environmental Science&Technology,2001,35(7):1339-1342
- [2]Kannan K,Tao L,Sinclair E,et al.Perfluorinated compounds in aquatic organisms at various trophic levels in a great lakes food chain[J].Archives of Environmental Contamination and Toxicology,2005,48(4):559-566
- [3]Yeung L W Y,Miyake Y,Sachi T,et al.Perfluorinated compounds and total and extractable organic fluorine in human blood samples from China[J].Environmental Science&Technology,2008,42(21):8140-8145
- [4]Naile J E,Khim J S,Hong S J,et al.Distributions and bioconcentration characteristics of perfluorinated compounds in environmental samples collected from the west coast of Korea[J].Chemosphere,2013,90(2):387-394
- [5]Tomy G T,Tittlemier S A,Palace V P,et al.Biotransformation of N-ethyl perfluorooctane sulfonamide by rainbow trout(Onchorhynchus mykiss)liver microsomes[J].Environmental Science&Technology,2004,38(3):758-762
- [6]Wang T,Wang Y,Liao C,et al.Perspectives on the inclusion of perfluorooctane sulfonate into the Stockholm Convention on persistent organic polutants[J].Environmental Science&Technology,2009,43(14):5171-5175
- [7]Moody C A,Martin J W,Kwan W C,et al.Monitoring perfluorinated surfactants in biota and surface water samples following an accidental release of fire-fighting foam into etobicoke creek[J].Environmental Science&Technology,2002,36(4):545-551
- [8]So M K,Miyake Y,Yeung W Y,et al.Perfluorinated compounds in the Pearl River and Yangtze River of China[J].Chemosphere,2007,68(11):2085-2095
- [9]Wang T Y,Lu Y L,Chen C L,et al.Perfluorinated compounds in estuarine and coastal areas of North Bohai Sea,China[J].Marine Pollution Buletin,2011,62(8):1905-1914
- [10]Bao J,Jin Y H,Liu W,et al.Perfluorinated compounds in sediments from the Daliao River system of Northeast China[J].Chemosphere,2009,77(5):652-657
- [11]Meng J,Wang T Y,Wang P,et al.Perfluoroalkyl substances and organochlorine pesticides in sediments from Huaihe Watershed in China[J].Journal of Environmental Sciences,2014,26(11):2198-2206
- [12]Boulanger B,Vargo J,Schnoor J L,et al.Detection of perfluorooctane surfactants in great lakes water[J].Environmental Science&Technology,2004,38(15):4064-4070
- [13]Naile J E,Khim J S,Wang T Y,et al.Perfluorinated compounds in water,sediment,soil and biota from estuarine and coastal areas of Korea[J].Environmental Pollution,2010,158(5):1237-1244
- [14]苏红巧,任东凯,曹闪,等.全氟辛烷磺酸盐(PFOS)及其替代品对两栖类蝌蚪的急性毒性[J].生态毒理学报,2012,7(5):521-524Su H,Ren D,Cao S,et al.Acute toxicity of perfluorooctane sulfonate(PFOS)and its substitutes to amphibian tadpoles[J].Asian Journal of Ecotoxicology,2012,7(5):521-524(in Chinese)
- [15]Kannan K,Hansen K J,Wade T L,et al.Perfluorooctane sulfonate in oysters,crassostrea virginica,from the Gulf of Mexico and the Chesapeake Bay,USA[J].Archives of Environmental Contamination and Toxicology,2002,42(3):313-318
- [16]Kowalczyk J,Ehlers S,Furst P,et al.Transfer of perfluorooctanoic acid(PFOA)and perfluorooctane sulfonate(PFOS)from contaminated feed into milk and meat of sheep:Pilot study[J].Archives of Environmental Contamination and Toxicology,2012,63(2):288-298
- [17]Chinje E,Kentish P,Jarnot B,et al.Induction of the cyp4a subfamily by perfluorodecanoic acid:The rat and the guinea pig as susceptible and non-susceptible species[J].Toxicology Letters,1994,71(1):69-75
- [18]Zhao H,Guan Y,Zhang G,et al.Uptake of perfluorooctane sulfonate(PFOS)by wheat(Triticum aestivum L.)plant[J].Chemosphere,2013,91(2):139-144
- [19]Felizeter S,Mc Lachlan M S,de Voogt P.Uptake of perfluorinated alkyl acids by hydroponically grown lettuce(Lactuca sativa)[J].Environmental Science&Technology,2012,46(21):11735-11743
- [20]Krippner J,Brunn H,Falk S,et al.Effects of chain length and p H on the uptake and distribution of perfluoroalkyl substances in maize(Zea mays)[J].Chemosphere,2014,94:85-90
- [21]Lechner M,Knapp H.Carryover of perfluorooctanoic acid(PFOA)and perfluorooctane sulfonate(PFOS)from soil to plant and distribution to the different plant compartments studied in cultures of carrots(Daucus carota ssp.Sativus),potatoes(Solanum tuberosum),and cucumbers(Cucumis sativus)[J].Journal of Agricultural and Food Chemistry,2011,59(20):11011-11018
- [22]Zhao H,Guan Y,Zhang G,et al.Uptake of perfluorooctane sulfonate(PFOS)by wheat[J].Chemosphere,2013,91(2):139-144
- [23]吕振娥,苏玉红,乔敏.全氟辛烷磺酸短期暴露对不同作物苗期生长的影响[J].生态毒理学报,2013,8(5):695-701Lv Z,Su Y,Qiao M.Influence of short-term exposure of PFOS on seedling growth of different plants[J].Asian Journal of Ecotoxicology,2013,8(5):695-701(in Chinese)
- [24]任珺,陶玲,杨倩,等.芦苇、菖蒲和水葱对水体中Cd富集能力的研究[J].农业环境科学学报,2010,29(9):1757-1762Ren J,Tao L,Yang Q,et al.Accumulation ability of Cd in water for Phragmites australis,Acorus calamus and Scirpus tabernaemontani[J].Journal of Agro-Environment Science,2010,29(9):1757-1762(in Chinese)
- [25]潘义宏,王宏镔,谷兆萍,等.大型水生植物对重金属的富集与转移[J].生态学报,2010,30(23):6430-6441Pan Y,Wang H,Gu Z,et al.Accumulation and translocation of heavy metals by macrophytes[J].Acta Ecologica Sinica,2010,30(23):6430-6441(in Chinese)
- [26]张蕾,周启星,孙福红,等.麝香酮和镉单一与复合污染对金鱼藻的生态毒理效应研究[J].生态毒理学报,2009,4(4):507-515Zhang L,Zhou Q,Sun F,et al.Single and joint effects of musk ketone and cadmium on coontail(Ceratophyllum demersum L.)[J].Asian Journal of Ecotoxicology,2009,4(4):507-515(in Chinese)
- [27]Monk L S,Fagerstedt K V,Crawford R M M.Oxygentoxicity and superoxide-dismutase as an antioxidant in physiological stress[J].Physiologia Plantarum,1989,76(3):456-459
- [28]Hanson M L,Sibley P K,Brain R A,et al.Microcosm evaluation of the toxicity and risk to aquatic macrophytes from perfluorooctane sulfonic acid[J].Archives of Environmental Contamination and Toxicology,2005,48(3):329-337
- [29]Lichtenthaler H K.Vegetation stress:An introduction to the stress concept in plants[J].Journal of Plant Physiology,1996,148(1-2):4-14
- [30]Borges T,Glauert H P,Robertson L W.Perfluorodecanoic acid noncompetitively inhibits the peroxisomal enzymes enoyl-Co A hydratase and 3-hydroxyacyl-Co A dehydrogenase[J].Toxicology and Applied Pharmacology,1993,118(1):8-15
- [31]顾晓军,田素芬.毒物兴奋效应概念及其生物学意义[J].毒理学杂志,2007(5):425-428
- [32]Zhou Q.Interaction between heavy metals and nitrogen fertilizers applied to soil-vegetable systems[J].Buletin of Environmental Contamination and Toxicology,2003,71(2):338-344
- [33]李砧,胡涛,李艳晖,等.金鱼藻对水中氟污染物去除作用的研究[J].广东农业科学,2013,40(18):165-168Li Z,Hu T,Li Y,et al.Research on removal effect of fluoride in water by Ceratophyllum demersum[J].Guangdong Agricultural Sciences,2013,40(18):165-168(in Chinese)