陈波;谷明娟;朱遥;刘勇亮;潘康成;

• 1:四川农业大学动物医学院动物微生态研究中心

摘要(Abstract)：

为了探讨苏云金芽孢杆菌(Bacillus thuringiensis,Bt)Bt Z01对小鼠胚胎发育和胚后生长发育的影响,将48只孕鼠随机分组,试验组饲喂浓度分别为106CFU·m L-1、108CFU·m L-1、5×109CFU·m L-1的Bt Z01菌液,并设灭菌水为对照组。采用行为致畸学方法检查Bt Z01对小鼠胚胎和胚后发育的影响并制作脏器切片,测量脏器指数等指标。结果表明,与对照组相比,各试验组小鼠在胚胎和胚后发育各阶段体重和体长无显著差异(P>0.05),胚胎畸形发生率无增加,骨骼染色观察发现高剂量组小鼠后囟门显著大于其他3组(P<0.05)。18 d与48 d小鼠部分脏器指数存在显著差异,但病理切片观察发现仅高剂量组肾脏有病变发生。试验结果表明,苏云金芽孢杆菌Bt Z01在浓度为108CFU·m L-1及以下时对小鼠胚胎发育及胚后生长发育无不良影响。

关键词(KeyWords)： 苏云金芽孢杆菌;小鼠;行为致畸学;胚胎发育;胚后发育

Abstract:

Keywords:

基金项目(Foundation): “教育部长江学者和创新团队发展计划”创新团队资助项目(IRT0848);; 四川农业大学创新性实验计划和科研兴趣培养计划(1310626013)

作者(Author): 陈波;谷明娟;朱遥;刘勇亮;潘康成;

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DOI:

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