高新磊;邵明非;贺小萌;欧阳峰;商丹丹;李继;

• 1:哈尔滨工业大学深圳研究生院深圳市水资源利用与环境污染控制重点实验室
• 2:北京国环清华环境工程设计研究院有限公司深圳分院

摘要(Abstract)：

考察了污水处理厂空气介质中典型的抗性基因(antibiotic resistance genes,ARGs)污染水平和浓度分布,并通过16S r RNA高通量技术对样品进行亲缘性及溯源研究。结果表明,在污水厂空气样品中8种抗生素抗性基因的检出率均超过50%,其中tet C、sul1、sul2和erm B检出率为100%。在曝气池和污泥脱水车间空气样品中8种抗性基因检出率均为100%。对其中的sul1、sul2、tet G和tet X共4种ARGs的定量分析结果表明,以上4种基因的相对浓度范围在102~105copies·ng~(-1)DNA之间,与邻近居民区空气样品抗性基因浓度处于同一水平;空气样品16S r RNA高通量测序聚类分析结果显示,居民区空气与污水厂园区内空气有较高的种群相似度,污水厂处理单元对其邻近区域的空气介质微生物组成影响较大。

关键词(KeyWords)： 抗生素抗性基因;空气介质微生物;污水厂

Abstract:

Keywords:

基金项目(Foundation): 环保公益性行业科研专项(201309031);; 国家自然科学基金(31200104);; 深圳市基础研究计划(JCYJ2012061315435096)

作者(Author): 高新磊;邵明非;贺小萌;欧阳峰;商丹丹;李继;

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