污水处理厂空气介质抗生素抗性基因的分布Distribution of Airborne Antibiotic Resistance Gene in Wastewater Treatment Plant
高新磊;邵明非;贺小萌;欧阳峰;商丹丹;李继;
摘要(Abstract):
考察了污水处理厂空气介质中典型的抗性基因(antibiotic resistance genes,ARGs)污染水平和浓度分布,并通过16S r RNA高通量技术对样品进行亲缘性及溯源研究。结果表明,在污水厂空气样品中8种抗生素抗性基因的检出率均超过50%,其中tet C、sul1、sul2和erm B检出率为100%。在曝气池和污泥脱水车间空气样品中8种抗性基因检出率均为100%。对其中的sul1、sul2、tet G和tet X共4种ARGs的定量分析结果表明,以上4种基因的相对浓度范围在102~105copies·ng~(-1)DNA之间,与邻近居民区空气样品抗性基因浓度处于同一水平;空气样品16S r RNA高通量测序聚类分析结果显示,居民区空气与污水厂园区内空气有较高的种群相似度,污水厂处理单元对其邻近区域的空气介质微生物组成影响较大。
关键词(KeyWords): 抗生素抗性基因;空气介质微生物;污水厂
基金项目(Foundation): 环保公益性行业科研专项(201309031);; 国家自然科学基金(31200104);; 深圳市基础研究计划(JCYJ2012061315435096)
作者(Author): 高新磊;邵明非;贺小萌;欧阳峰;商丹丹;李继;
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