纳米硫化镉量子点细胞毒性作用机制The Cytotoxicity Mechanism of Unmodified Cadmium Sulfide Quantum Dots
余强;李坤刚;文兴;李君;王玉秋;
摘要(Abstract)：
为初步探讨硫化镉量子点(CdS QDs)的细胞毒性作用机制,采用MTT毒性实验比较了CdS QDs和常规CdS对仓鼠肺细胞(CHL)的毒性效应以及细胞内外活性氧水平.结果表明,1)在较低暴露浓度(≤20μg·mL-1)时,CdS QDs细胞毒性显著高于常规CdS,而在较高暴露浓度(>20μg·mL-1)时,两者相差不大.2)在较低暴露浓度(≤40μg·mL-1)时,添加N-乙酰半胱氨酸(NAC)可显著降低CdS QDs的细胞毒性,而在较高暴露浓度(>40μg·mL-1)时,添加NAC对CdS QDs的细胞毒性没有明显影响.添加NAC对常规CdS细胞毒性没有显著影响.综合实验结果推测CdS QDs的细胞毒性与暴露剂量有关:在低浓度(<20μg·mL-1)时,主要是活性氧的氧化损伤作用;在中等浓度(20~40μg·mL-1)时,活性氧和Cd2+的释放共同作用;在高浓度(>40μg·mL-1)时,则是Cd2+的释放占主导地位.
关键词(KeyWords)： 硫化镉;量子点;细胞毒性;活性氧
基金项目(Foundation): 南开大学本科生创新科研百项工程项目(No.06064);; 教育部博士点基金(No.200800550011)
作者(Author): 余强;李坤刚;文兴;李君;王玉秋;
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DOI:
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