纳米硫化镉量子点细胞毒性作用机制The Cytotoxicity Mechanism of Unmodified Cadmium Sulfide Quantum Dots
余强;李坤刚;文兴;李君;王玉秋;
摘要(Abstract)：
为初步探讨硫化镉量子点(CdS QDs)的细胞毒性作用机制,采用MTT毒性实验比较了CdS QDs和常规CdS对仓鼠肺细胞(CHL)的毒性效应以及细胞内外活性氧水平.结果表明,1)在较低暴露浓度(≤20μg·mL-1)时,CdS QDs细胞毒性显著高于常规CdS,而在较高暴露浓度(>20μg·mL-1)时,两者相差不大.2)在较低暴露浓度(≤40μg·mL-1)时,添加N-乙酰半胱氨酸(NAC)可显著降低CdS QDs的细胞毒性,而在较高暴露浓度(>40μg·mL-1)时,添加NAC对CdS QDs的细胞毒性没有明显影响.添加NAC对常规CdS细胞毒性没有显著影响.综合实验结果推测CdS QDs的细胞毒性与暴露剂量有关:在低浓度(<20μg·mL-1)时,主要是活性氧的氧化损伤作用;在中等浓度(20~40μg·mL-1)时,活性氧和Cd2+的释放共同作用;在高浓度(>40μg·mL-1)时,则是Cd2+的释放占主导地位.
关键词(KeyWords)： 硫化镉;量子点;细胞毒性;活性氧
基金项目(Foundation): 南开大学本科生创新科研百项工程项目(No.06064);; 教育部博士点基金(No.200800550011)
作者(Author): 余强;李坤刚;文兴;李君;王玉秋;
Email:
参考文献(References)：
- Bai S S,Li Q Z,Liu K M,Zhang J S,Liu L,Gao J K,Wang Y Q.2008.Comparative study of genotoxicity in miceexposed to cadmium sulfide quantum dots and normal cadmiumsulfide[J].Asian Journal of Ecotoxicology,3(1):59-64(inChinese)
- Cho S J,Maysinger D,Jain M,Rder B,Hackbarth S,WinnikF M.2007.Long-term exposure to CdTe quantum dots causesfunctional impairments in live cells[J].Langmuir,23(4):1974-1980
- Choi A O,Cho S J,Desbarats J,Lovric'J,Maysinger D.2007.Quantum dot-induced cell death involves Fas upregulation andlipid peroxidation in human neuroblastoma cells[J].Journal ofNanobiotechnology,5:1
- Clarke S J,Hollmann C A,Zhang Z,Suffern D,Bradforth S E,Dimitrijevic N M,Minarik W G,Nadeau J L.2006.Photophysicsof dopamine-modified quantum dots and effects on biologicalsystems[J].Nature Materials,5(5):409-417
- Derfus A M,Chan W C W,Bhatia S N.2004.Probing thecytotoxicity of semiconductor quantum dots[J].Nano Letters,4(1):11-18
- Finkel T,Holbrook N J.2000.Oxidants,oxidative stress and thebiology of ageing[J].Nature,408(6809):239-247
- Green M,Howman E.2005.Semiconductor quantum dots andfree radical induced DNA nicking[J].Chemical Communications(Cambridge,England)(,1):121-123
- Hoshino A,Fujioka K,Oku T,Suga M,Sasaki Y F,Ohta T,Yasuhara M,Suzuki K,Yamamoto K.2004.Physicochemicalproperties and cellular toxicity of nanocrystal quantum dotsdepend on their surface modification[J].Nano Letters,4(11):2163-2169
- Ipe B I,Lehnig M,Niemeyer C M.2005.On the generation offree radical species from quantum dots[J].Small,1(7):706-709
- Jamieson T,Bakhshi R,Petrova D,Pocock R,Imani M,Seifalian A M.2007.Biological applications of quantum dots[J].Biomaterials,28(31):4717-4732
- Kirchner C,Javier A M,Susha A S,Rogach A L,Kreft O,Sukhorukov G B,Parak W J.2005.Cytotoxicity of nanoparticle-loaded polymer capsules[J].Talanta,67(3):486-491
- Larson D R,Zipfel W R,Williams R M,Clark S W,BruchezM P,Wise F W,Webb W W.2003.Water-soluble quantumdots for multiphoton fluorescence imaging in vivo[J].Science,300(5624):1434-1436
- Lovric'J,Cho S J,Winnik F M,Maysinger D.2005.Unmodifiedcadmium telluride quantum dots induce reactive oxygen speciesformation leading to multiple organelle damage and cell death[J].Chemistry&Biology,12(11):1227-1234
- Shiohara A,Hoshino A,Hanaki K,Suzuki K,Yamamoto K.2004.On the cyto-toxicity caused by quantum dots[J].Microbiologyand Immunology,48(9):669-675
- Tsay J M,Michalet X.2005.New light on quantum dot cytotoxicity[J].Chemistry&Biology,12(11):1159-1161
- Ye H Q,Yang X L,Zhou J Y,Xu H B.2001.The researchprogress of toxicity mechanism of environmental pollutant ofcadmium[J].Guangdong Trace Elements Science,8:9-12(inChinese)
- Yin F R,Gao Y X,Chen C Y,Wu G.2008.Toxicologicalstudy of quantum dots[J].Journal of Hygiene Research,37(6):760-763(in Chinese)
- 柏杉山,李清钊,刘克明,张静姝,刘璐,高军凯,王玉秋.2008.硫化镉量子点与常规硫化镉对小鼠遗传毒性的比较研究[J].生态毒理学报,3(1):59-64
- 叶寒青,杨祥良,周井炎,徐辉碧.2001.环境污染物镉毒性作用机理研究进展[J].广东微量元素科学,8:9-12
- 尹芳蕊,高愈希,陈春英,吴刚.2008.量子点的毒理学研究进展[J].卫生研究,37(6):760-763