王斐;黄益宗;王小玲;高柱;余发新;徐峰;保琼莉;胡莹;乔敏;金姝兰;黄永春;李季;向猛;

• 1:中国科学院生态环境研究中心
• 2:农业部环境保护科研监测所
• 3:江西省科学院生物资源研究所
• 4:上饶师范学院

摘要(Abstract)：

为了研究铜矿周边农田土壤中不同Cu含量对土壤酶活性、细菌和古菌基因拷贝数的影响,在铜矿周边农田土壤中采集了8个不同Cu含量的土壤,测定土壤的酶活性、细菌和古菌基因拷贝数,结果表明,土壤总Cu含量为28.30~1 019.27mg·kg~(-1),酸可提取态Cu含量为335~415.11 mg·kg~(-1),细菌16S rRNA基因拷贝数在每克干土3.10×10~(10)~1.84×10~(11)个之间,古茵16S rRNA基因拷贝数在每克干土737×10~8~6.82×10~9个之间。土壤总Cu和酸可提取态Cu含量对土壤脲酶活性影响较大,但对土壤脱氢酶和转化酶活性影响不显著。土壤细菌基因拷贝数均与总Cu含量、各形态Cu含量呈极显著的负相关关系,同样土壤古菌基因拷贝数与总Cu含量、各形态Cu含量也均呈极显著的负相关关系,说明Cu胁迫对土壤细菌和古菌活性具有较大的抑制作用。

关键词(KeyWords)： real-time PCR;铜;细菌;古菌;脲酶;脱氢酶;转化酶

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(21377152);; 江西省科学院省级重点实验室开放基金项目(2012-KLB-1和2013-KLB-08)资助

作者(Author): 王斐;黄益宗;王小玲;高柱;余发新;徐峰;保琼莉;胡莹;乔敏;金姝兰;黄永春;李季;向猛;

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