程艳;崔媛;何平;谢文平;殷缓缓;周新;于文莲;李海山;陈会明;

• 1:中国检验检疫科学研究院
• 2:中国合格评定国家认可中心

摘要(Abstract)：

为了研究低剂量的全氟辛烷磺酸(perfluorooctane sulfonate,PFOS)对水生生物的内分泌干扰效应和作用机制,考察了PFOS对斑马鱼(Brachydanio rerio)血浆和组织匀浆中卵黄蛋白原(vitellogenin,VTG)含量的影响。将雄性和雌性斑马鱼分别暴露于0.1、1、10和100μg·L-1的PFOS中进行21d毒性实验,染毒结束后分别检测雄鱼和雌鱼的血浆、头尾组织匀浆液和全鱼匀浆液中的VTG含量。结果显示:(1)PFOS暴露可引起斑马鱼血浆、全鱼和头尾匀浆液中VTG含量的升高,VTG含量的排序为雌鱼(血浆>>全鱼匀浆>头尾匀浆)>>雄鱼(血浆>全鱼匀浆>>头尾匀浆);(2)PFOS暴露所引起的雄鱼血浆和头尾匀浆中VTG含量的升高与剂量呈负相关关系,暴露浓度为0.1μg·L-1时与对照组相比有显著性差异(p<0.05);(3)雌鱼血浆和头尾匀浆中VTG含量的升高与剂量呈倒U型曲线关系,暴露浓度为10μg·L-1时与对照组相比有显著性差异(p<0.05);(4)雄鱼和雌鱼的全鱼匀浆液中的VTG含量与对照组相比均无显著性差异。研究结果表明,PFOS暴露对斑马鱼的内分泌干扰作用明显,其毒性作用机制可能是类雌激素效应,血液和头尾匀浆液中VTG含量能够作为PFOS内分泌干扰效应评价的敏感生物标志物,但响应曲线可能因性别和组织部位的不同有所差异。

关键词(KeyWords)： 全氟辛烷磺酸;斑马鱼;卵黄蛋白原

Abstract:

Keywords:

基金项目(Foundation): 国家质量监督检验检疫总局课题“化学物质内分泌干扰危害效应评价研究”(2010IK030);国家质量监督检验检疫总局2011年公益项目“化学品安全评价替代方法和本土生物模型评价关键技术标准研究”(201110250);国家质量监督检验检疫总局2011年公益项目“进出口消费品及化学品中聚合物的检测及风险评估技术研究”(201110020);; 2012年中国检验检疫科学研究院专项基金“斑马鱼胚胎吸入暴露的生长发育毒性机制研究”(2012JK026);; 科技部科研院所技术专项“典型全氟碳化合物PFCs和挥发性化学物质风险测试关键技术研究”(2010EG12847)

作者(Author): 程艳;崔媛;何平;谢文平;殷缓缓;周新;于文莲;李海山;陈会明;

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