程艳;崔媛;何平;谢文平;殷缓缓;周新;于文莲;李海山;陈会明;

• 1:中国检验检疫科学研究院
• 2:中国合格评定国家认可中心

摘要(Abstract)：

为了研究低剂量的全氟辛烷磺酸(perfluorooctane sulfonate,PFOS)对水生生物的内分泌干扰效应和作用机制,考察了PFOS对斑马鱼(Brachydanio rerio)血浆和组织匀浆中卵黄蛋白原(vitellogenin,VTG)含量的影响。将雄性和雌性斑马鱼分别暴露于0.1、1、10和100μg·L-1的PFOS中进行21d毒性实验,染毒结束后分别检测雄鱼和雌鱼的血浆、头尾组织匀浆液和全鱼匀浆液中的VTG含量。结果显示:(1)PFOS暴露可引起斑马鱼血浆、全鱼和头尾匀浆液中VTG含量的升高,VTG含量的排序为雌鱼(血浆>>全鱼匀浆>头尾匀浆)>>雄鱼(血浆>全鱼匀浆>>头尾匀浆);(2)PFOS暴露所引起的雄鱼血浆和头尾匀浆中VTG含量的升高与剂量呈负相关关系,暴露浓度为0.1μg·L-1时与对照组相比有显著性差异(p<0.05);(3)雌鱼血浆和头尾匀浆中VTG含量的升高与剂量呈倒U型曲线关系,暴露浓度为10μg·L-1时与对照组相比有显著性差异(p<0.05);(4)雄鱼和雌鱼的全鱼匀浆液中的VTG含量与对照组相比均无显著性差异。研究结果表明,PFOS暴露对斑马鱼的内分泌干扰作用明显,其毒性作用机制可能是类雌激素效应,血液和头尾匀浆液中VTG含量能够作为PFOS内分泌干扰效应评价的敏感生物标志物,但响应曲线可能因性别和组织部位的不同有所差异。

关键词(KeyWords)： 全氟辛烷磺酸;斑马鱼;卵黄蛋白原

Abstract:

Keywords:

基金项目(Foundation): 国家质量监督检验检疫总局课题“化学物质内分泌干扰危害效应评价研究”(2010IK030);国家质量监督检验检疫总局2011年公益项目“化学品安全评价替代方法和本土生物模型评价关键技术标准研究”(201110250);国家质量监督检验检疫总局2011年公益项目“进出口消费品及化学品中聚合物的检测及风险评估技术研究”(201110020);; 2012年中国检验检疫科学研究院专项基金“斑马鱼胚胎吸入暴露的生长发育毒性机制研究”(2012JK026);; 科技部科研院所技术专项“典型全氟碳化合物PFCs和挥发性化学物质风险测试关键技术研究”(2010EG12847)

作者(Author): 程艳;崔媛;何平;谢文平;殷缓缓;周新;于文莲;李海山;陈会明;

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参考文献(References)：

• [1]Tilghman S L,Nierth-Simpson E N,Wallace R,et al.Environmental hormones:Multiple pathways for response may lead to multiple disease outcomes[J].Steroids,2010,75(8-9):520-523
• [2]United Nations.Globally Harmonised System of Classifi-cation and Labeling of Chemicals(GHS)[R].Rio de Janeiro,Brazil:UN,1992
• [3]European Union.Registration,Evaluation,Authorisati-on and Restriction of Chemicals(REACH)[R].Hel-sinki,Finland:EU,2007
• [4]Clarke B O,Smith S R.Review of'emerging'organic con-taminants in biosolids and assessment of international re-search priorities for the agricultural use of biosolids[J].Environment International,2011,37(1):226-247
• [5]EU.Directive2006/122/EC of the European Parliament and of the Council of12December2006Amending for the30th Time Council Directive76/769/EEC on the Approximation of the Laws,Regulations and Administra-tive Provisions of the Member States Relating to Restric-tions on the Marketing and Use of Certain Dangerous Substances and Preparations(Perfluorooctane Sulfon-ates)[R].Strasbourg,France:EU,2006
• [6]Jensen A A,Leffers H.Emerging endocrine disrupters:Perfluoroalkylated substances[J].International Journal of Andrology,2008,31(2):161-169
• [7]Martin M T,Brennan R J,Hu W,et al.Toxicogenomic study of triazole fungicides and perfluoroalkyl acids in rat livers predicts toxicity and categorizes chemicals based on mechanisms of toxicity[J].Toxicological Sci-ences,2007,97(2):595-613
• [8]Thibodeaux J R,Hanson R G,Rogers J M,et al.Ex-posure to perfluorooctane sulfonate during pregnancy in rat and mouse.I:Maternal and prenatal evaluations[J].Toxicological Sciences,2003,74(2):369-381
• [9]Lau C,Thibodeaux J R,Hanson R G,et al.Exposure to perfluorooctane sulfonate during pregnancy in rat and mouse.II:Postnatal evaluation[J].Toxicological Sci-ences,2003,74(2):382-392
• [10]Chang S C,Thibodeaux J R,Eastvold M L,et al.Neg-ative bias from analog methods used in the analysis of free thyroxine in rat serum containing perfluorooctane sulfonate(PFOS)[J].Toxicology,2007,234(1-2):21-33
• [11]Austin M E,Kasturi B S,Barber M,et al.Neuroendo-crine effects of perfluorooctane sulfonate in rats[J].Environmental Health Perspectives,2003,111(12):1485-1489
• [12]OECD.OECD Guideline for the Testing of Chemicals,OECD TG230.21-Day Fish Assay:A Short-Term Screening for Oestrogenic and Androgenic Activity,and Aromatase Inhibition[R].Paris,France:OECD,2009
• [13]Matozzo V,Gagn F,Marin M G,et al.Vitellogenin as a biomarker of exposure to estrogenic compounds in a-quatic invertebrates:A review[J].Environment Inter-national,2008,34(4):531-545
• [14]Andersen L,Goto-Kazeto R,Trant J M,et al.Short-term exposure to low concentrations of the synthetic an-drogen methyltestosterone affects vitellogenin and steroid levels in adult male zebrafish(Danio rerio)[J].A-quatic Toxicology,2006,76(3-4):343-352
• [15]Du Y B,Shi X J,Liu C S,et al.Chronic effects of wa-ter-borne PFOS exposure on growth,survival and hepa-totoxicity in zebrafish:A partial life-cycle test[J].Chemosphere,2009,74(5):723-729
• [16]Oakes K D,Sibley P K,Martin J W,et al.Short-term exposures of fish to perfluorooctane sulfonate:Acute effects on fatty acyl-CoA oxidase activity,oxidative stress,and circulating sex steroids[J].Environmental Toxicology and Chemistry,2005,24(5):1172-1181
• [17]Han J,Fang Z Q.Estrogenic effects,reproductive im-pairment and developmental toxicity in ovoviparous swordtail fish(Xiphophorus helleri)exposed to perfluo-rooctane sulfonate(PFOS)[J].Aquatic Toxicology,2010,99(2):281-290
• [18]Beach S A,Newsted J L,Coady K,et al.Ecotoxicolog-ical evaluation of perfluorooctane sulfonate(PFOS)[J].Reviews of Environmental Contamination and Toxi-cology,2006,186:133-174
• [19]廖涛,徐盈,钟雪萍,等.EE2对稀有鮈鲫和斑马鱼幼鱼体内卵黄蛋白原诱导的比较[J].水生生物学报,2005,29(5):513-517Liao T,Xu Y,Zhong X P,et al.Comparative vitellogen-ic responses in zebrafish(Brachydanio rerio)and rare minnow(Gobiocypris rarus)exposed to17α-ethi-nylestradiol[J].Acta Hydrobiologica Sinica,2005,29(5):513-517(in Chinese)
• [20]李洁斐,李卫华,杨健,等.17-α炔雌醇对斑马鱼的卵黄蛋白原及性腺发育的影响[J].毒理学杂志,2006,20(1):9-13Li J F,Li W H,Yang J,et al.Effects of17-α-ethy-nylestradiol on vitellogenin and gonad development of ze-brafish[J].Journal of Toxicology,2006,20(1):9-13(in Chinese)