刘冰;金一和;董光辉;刘薇;齐藤宪光;佐佐木和明;

• 1:中国医科大学公共卫生学院
• 2:中国医科大学公共卫生学院
• 3:中国医科大学公共卫生学院
• 4:大连理工大学环境与生命学院工业生态与环境工程教育部重点实验室

摘要(Abstract)：

采用连续灌胃染毒的方法,探讨了全氟辛酸(Perfluorooctanoic acid,PFOA)经口急性染毒对大鼠海马细胞内钙离子浓度的影响.选择雄性Wistar大鼠40只,实验组PFOA染毒剂量分别为2、8、30mg·kg-1(bw).连续灌胃染毒7天后,制备海马单细胞悬液,采用Fura-2/AM荧光探针法测定海马细胞内游离钙离子浓度([Ca2+]i),使用固相萃取-高效液相色谱/质谱联机法(HPLC/MS-MIS)检测血清与脑组织中PFOA浓度.结果表明,染毒组大鼠血清与脑中PFOA浓度均显著高于对照组水平(p<0.01),血清与脑中PFOA浓度之间存在显著的正相关关系(r2=0.611,p<0.01).PFOA染毒8、30mg·kg-1(bw)实验组海马细胞[Ca2+]i分别为(207.89±22.84)nmol·L-1和(284.19±14.75)nmol·L-1,显著高于对照组((141.68±11.47)nmol·L-1)和PFOA染毒2mg·kg-1(bw)实验组((147.38±19.23)nmol·L-1)(p<0.01).大鼠脑、血清中PFOA浓度分别与海马[Ca2+]i存在正相关关系(r2=0.552,p<0.01;r2=0.756,p<0.01).研究结果显示,PFOA暴露可使血清和脑组织中PFOA浓度增加,引起大鼠海马神经元细胞[Ca2+]i升高.

关键词(KeyWords)： PFOA;大鼠;海马;细胞内钙离子浓度

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(No.30471435);; 辽宁省教育厅项目(No.2004C027)

作者(Author): 刘冰;金一和;董光辉;刘薇;齐藤宪光;佐佐木和明;

Email:

DOI:

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