高上吉;钟晓霞;刘婉莹;王勉之;孙静;李弘程;孙永学;

• 1:华南农业大学广东省兽药研制与安全评价重点实验室

摘要(Abstract)：

为评价洛克沙胂的遗传毒性,采用单细胞凝胶电泳(彗星实验),研究了不同浓度的洛克沙胂对秀丽隐杆线虫胚胎细胞脱氧核醣核酸(DNA)的损伤作用。提取秀丽隐杆线虫的胚胎细胞,分别暴露于0(空白对照)、50、250、500μg·L~(-1)含洛克沙胂的溶液染毒1 h。用彗尾DNA百分比含量(TDNA%)、彗星尾长(TL)和Olive尾矩(OTM)作为DNA损伤的指标。实验结果表明,与空白对照组比较,处理组中彗尾DNA百分比含量、彗星尾长以及Olive尾矩显著增加(P<0.01)。随着洛克沙胂浓度的增加,彗尾DNA百分比含量、彗星尾长以及Olive尾矩逐渐增加,其相关系数r>0.99,说明在实验浓度范围内,存在极显著的浓度-效应关系。洛克沙胂对秀丽隐杆线虫胚胎细胞DNA具有损伤作用,彗星实验操作简便、快速、灵敏度高,能够反映出洛克沙胂的遗传毒性。因此,通过彗星实验建立实验室检测洛克沙胂遗传毒性的方法具有可行性。

关键词(KeyWords)： 洛克沙胂;秀丽隐杆线虫;彗星实验;DNA损伤;遗传毒性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31172368)

作者(Author): 高上吉;钟晓霞;刘婉莹;王勉之;孙静;李弘程;孙永学;

Email:

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