赵丽红;朱小山;王一翔;晋慧;王江新;王超;劳永民;姜玥璐;陶益;周进;蔡中华;

• 1:清华大学环境学院
• 2:清华大学深圳研究生院海洋学部
• 3:清华大学生命学院
• 4:深圳大学生命学院

摘要(Abstract)：

纳米二氧化钛(n Ti O2)在被人们广泛使用的同时,其潜在的环境影响也受到越来越多的关注。为深入探讨n Ti O2与环境中现有污染物的相互作用及生物效应,以斜生栅藻(Scenedesmus obliquus)为受试生物,按照毒性单位法、相加指数法和混合毒性指数法,研究了n Ti O2与双酚A(BPA,一种常见的环境类雌激素)的联合毒性效应。结果显示,n Ti O2与BPA对S.obliquus生长的72 h半抑制浓度(EC50)分别为28.7 mg·L-1与1.81 mg·L-1。而n Ti O2与BPA共存时,在不同毒性比(4:1,3:1,2:1,1:1,1:2和1:3)下,其联合毒性作用(以BPA计)的72 h EC50值分别为2.198,1.58,1.153,0.428,0.306和0.189 mg·L-1。两者的联合毒性作用不仅仅是简单的相加,而是随着两者毒性比的变化,由拮抗作用转变为相加作用,继而转变为协同作用。这表明,n Ti O2进入环境后与现有污染物的毒性比(浓度比)可能是其联合毒性作用模式的一个重要影响因素。

关键词(KeyWords)： 纳米二氧化钛;双酚A;斜生栅藻;联合毒性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(21107058;41373089);; 环境污染过程与基准教育部重点实验室(南开大学)开放基金资助项目(KLPPEC-2013-01)

作者(Author): 赵丽红;朱小山;王一翔;晋慧;王江新;王超;劳永民;姜玥璐;陶益;周进;蔡中华;

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参考文献(References)：

• [1]Sharma V K.Aggregation and toxicity of titanium dioxide nanoparticles in aquatic environment-A Review[J].Journal of Environmental Science and Health Part A,2009,44(14):1485-1495
• [2]Colvin V L.The potential environmental impact of engineered nanomaterials[J].Nature Biotechnology,2003,21(10):1166-1170
• [3]汪冰,丰伟悦,赵宇亮,等.纳米材料生物效应及其毒理学研究进展[J].中国科学(B辑化学),2005(1):1-10Wang B,Feng W Y,Zhao Y L,et al.The research progress of nanomateriaks biological effect and toxicology[J].Science in China(Series B:Chemistry),2005(1):1-10(in Chinese)
• [4]朱小山,朱琳.人工纳米材料生物效应研究进展[J].安全与环境学报,2005(4):86-90(in Chinese)Zhu X S,Zhu L.Review on biological effects of manufactured nano-materials[J].Journal of Safety and Environment,2005(4):86-90(in Chinese)
• [5]张娜,高霞,杜宏,等.双酚A及纳米二氧化钛对人胚肝L-02细胞内活性氧含量及DNA损伤的联合作用[J].环境与职业医学,2008(4):356-359Zhang N,Gao X,Du H,et al.Combined effects of bisphenol A and nano-Ti O2on ROS and DNA damage level in human embryo liver L-02 cells[J].Journal of Environmental&Occupational Medicine,2008(4):356-359(in Chinese)
• [6]Zheng D,Wang N,Wang X,et al.Effects of the interaction of Ti O2nanoparticles with bisphenol A on their physicochemical properties and in vitro toxicity[J].Journal of Hazardous Materials,2012,199-200:426-432
• [7]Yan J,Lin B C,Hu C L,et al.The combined toxicological effects of titanium dioxide nanoparticles and bisphenol A on zebrafish embryos[J].Nanoscale Research Letters,2014,9(1):1-9
• [8]李锋民,赵薇,李媛媛,等.纳米Ti O2对短裸甲藻的毒性效应[J].环境科学,2012(1):233-238Li F M,Zhao W,Li Y Y,et al.Toxic effect of nano-Ti O2on Gymnodinium breve[J].Chinese Journal of Environmental Science,2012(1):233-238(in Chinese)
• [9]Kaegi R,Ulrich A,Sinnet B,et al.Synthetic Ti O(2)nanoparticle emission from exterior facades into the aquatic environment[J].Environmental Pollution,2008,156(2):233-239
• [10]Muel er N C,Nowack B.Exposure modeling of engineered nanoparticles in the environment[J].Environmental Science&Technology,2008,42(12):4447-4453
• [11]刘美婷,余冉,陈良辉,等.典型纳米金属氧化物对氨氧化菌Nitrosomonas europaea的生物胁迫影响[J].中国环境科学,2015(1):35Liu M T,Yu R,Chen L H,et al.Biological effect of typical metal oxide nanoparticles on Nitrosomonas europaea[J].China Environmental Science,2015(1):35(in Chinese)
• [12]Aruoja V,Dubourguier H C,Kasemets K,et al.Toxicity of nanoparticles of Cu O,Zn O and Ti O2to microalgae Pseudokirchneriella subcapitata[J].Science of the Total Environment,2009,407(4):1461-1468
• [13]Hartmann N B,Von der Kammer F,Hofmann T,et al.Algal testing of titanium dioxide nanoparticles-Testing considerations,inhibitory effects and modification of cadmium bioavailability[J].Toxicology,2010,269(2-3):190-197
• [14]Lovern S B,Klaper R.Daphnia magna mortality when exposed to titanium dioxide and fullerene(C-60)nanoparticles[J].Environmental Toxicology and Chemistry,2006,25(4):1132-1137
• [15]Lovern S B,Strickler J R,Klaper R.Behavioral and physiological changes in Daphnia magna when exposed to nanoparticle suspensions(titanium dioxide,nano-C-60,and C(60)Hx C(70)Hx)[J].Environmental Science&Technology,2007,41(12):4465-4470
• [16]Federici G,Shaw B J,Handy R D.Toxicity of titanium dioxide nanoparticles to rainbow trout(Oncorhynchus mykiss):Gil injury,oxidative stress,and other physiological effects[J].Aquatic Toxicology,2007,84(4):415-430
• [17]Johnston B D,Scown T M,Moger J,et al.Bioavailability of nanoscale metal oxides Ti O2,Ce O2,and Zn O to fish[J].Environmental Science&Technology,2010,44(3):1144-1151
• [18]Balbi T,Smerilli A,Fabbri R,et al.Co-exposure to n-Ti O2and Cd2+results in interactive effects on biomarker responses but not in increased toxicity in the marine bivalve M.gal oprovincialis[J].Science of the Total Environment,2014,493:355-364
• [19]Zhu X S,Chang Y,Chen Y S.Toxicity and bioaccumulation of Ti O2nanoparticle aggregates in Daphnia magna[J].Chemosphere,2010,78(3):209-215
• [20]Zhu X S,Zhu L,Duan Z H,et al.Comparative toxicity of several metal oxide nanoparticle aqueous suspensions to Zebrafish(Danio rerio)early developmental stage[J].Journal of Environmental Science and Health Part A,2008,43(3):278-284
• [21]Zhu X S,Zhou J,Cai Z H.Ti O2nanoparticles in the marine environment:Impact on the toxicity of tributyltin to abalone(Haliotis diversicolor supertexta)embryos[J].Environmental Science&Technology,2011,45(8):3753-3758
• [22]Tian S Y,Zhang Y D,Song C Z,et al.Titanium dioxide nanoparticles as carrier facilitate bioaccumulation of phenanthrene in marine bivalve,ark shell(Scapharca subcrenata)[J].Environental Pollution,2014,192:59-64
• [23]Zhang X Z,Sun H W,Zhang Z Y,et al.Enhanced bioaccumulation of cadmium in carp in the presence of titanium dioxide nanoparticles[J].Chemosphere,2007,67(1):160-166
• [24]Yang W W,Miao A J,Yang L Y.Cd2+toxicity to a green alga Chlamydomonas reinhardtii as influenced by its adsorption on Ti O2engineered nanoparticles[J].Plos One,2012,7(3):e32300
• [25]Yang W W,Li Y,Miao A J,et al.Cd2+toxicity as affected by bare Ti O2nanoparticles and their bulk counterpart[J].Ecotoxicology and Environmental Safety,2012,85:44-51
• [26]辛元元,陈金媛,程艳红,等.纳米Ti O2与重金属Cd对铜绿微囊藻生物效应的影响[J].生态毒理学报,2013,8(1):23-28Xin Y Y,Chen J Y,Cheng Y H,et al.Ecological effects of nano-Ti O2and heavy metal Cd on M.aeruginosa[J].Asian Journal of Ecotoxicology,2013,8(1):23-28(in Chinese)
• [27]Bliss C I.The toxicity of poisons applied jointly[J].Annals of Applied Biology,1939,26(3):585-615
• [28]Nirmalakhandan N,Arulgnanendran V,Mohsin M,et al.Toxicity of mixtures of organic-chemicals to microorganisms[J].Water Research,1994,28(3):543-551
• [29]Sprague J,Ramsay B A.Lethal levels of mixed copperzinc solutions for juvenile salmon[J].Journal of the Fisheries Board of Canada,1965,22(2):425-432
• [30]王焕校.污染生态学[M].北京:高等教育出版社,2000:50-55Wang H X.Pollution Ecology[M].Beijing:China Higher Education Press,2000:50-55(in Chinese)
• [31]Knemann H.Fish toxicity tests with mixtures of more than two chemicals:A proposal for a quantitative approach and experimental results[J].Toxicology,1981,19(3):229-238
• [32]Marking L.Method for assessing additive toxicity of chemical mixtures[J].Aquatic Toxicology and Hazard Evaluation,1977,634:99-108
• [33]OECD.Guidelines for the Testing of Chemicals.Method201,Freshwater Alga and Cyanobateria,Growth Inhibition Test[M].Organization for Economic Co-operation and Development,1994
• [34]朱小山,朱琳,田胜艳,等.三种金属氧化物纳米颗粒的水生态毒性[J].生态学报,2008,28(8):3507-3516Zhu X S,Zhu L,Tian S Y,et al.Aquatic ecotoxicities of nanoscales Ti O2,Zn O and Al2O3water suspensions[J].Acta Ecologica Sinica,2008,28(8):3507-3516(in Chinese)
• [35]朱小山.几种人工纳米材料的生态毒理学研究[D].天津:南开大学,2007Zhu X S.Aquatic ecotoxicities of serveral manufactured nanomaterials[D].Tianjin:Nankai University,2007
• [36]Sadiq I M,Dalai S,Chandrasekaran N,et al.Ecotoxicity study of titania(Ti O2)NPs on two microalgae species:Scenedesmus sp and Chlorella sp.[J].Ecotoxicology and Environmental Safety,2011,74(5):1180-1187
• [37]Menard A,Drobne D,Jemec A.Ecotoxicity of nanosized Ti O2.Review of in vivo data[J].Environmental Pollution,2011,159(3):677-684
• [38]贾秀英.镉对泥鳅幼鱼的急性和亚急性毒性研究[J].环境污染与防治,2001,23(5):227-228Jia X Y.Acute and subacute toxicity of cadmium on juvenile loach[J].Environmental Pollution and Control,2001,23(5):227-228(in Chinese)
• [39]Staples C A,Dorn P B,Klecka G M,et al.A review of the environmental fate,effects,and exposures of bisphenol A[J].Chemosphere,1998,36(10):2149-2173
• [40]Baun A,Srensen S N,Rasmussen R,et al.Toxicity and bioaccumulation of xenobiotic organic compounds in the presence of aqueous suspensions of aggregates of nanoC60[J].Aquatic Toxicology,2008,86(3):379-387
• [41]晏晓敏,查金苗,石宝友,等.纳米富勒烯聚集体与阿特拉津复合体系对水生生物的毒性[J].科学通报,2009,54(23):3612-3618Yan X M,Zha J M,Shi B Y,et al.In vivotoxicity of nanoC60aggregates complex with atrazine to aquatic organisms[J].Chinese Science Bul etin,2009,54(23):3612-3618(in Chinese)
• [42]高霞,张娜,杜宏,等.纳米二氧化钛对氯化镉所致人胚肝L-02细胞毒性作用的影响[J].环境与职业医学,2008,25(3):256-258Gao N,Zhang N,Du H,et al.Influence of nano-Ti O2on the toxic effects of cadmium chloride in human embryo liver L-02 cells[J].Journal of Environmental&Occupational Medicine,2008,25(3):256-258(in Chinese)
• [43]石丹.纳米四氧化三铁和镉联合作用对人肝癌Hep G2细胞DNA损伤的影响[D].武汉:华中科技大学,2006Shi D.Effects of nano-Fe3O4and cadmium on DNA damage in human hepatoma(Hep G2)cells[D].Wuhan:Huazhong University of Science and Technology,2006(in Chinese)
• [44]王壮,陈景文,邱文,等.典型纳米颗粒物和有机污染物对蛋白核小球藻的联合毒性[C].第五届全国环境化学大会摘要集,2009Wang Z,Chen J W,Qiu W,et al.The combined effects of typical nanoparticles and organic pol utions on Chlorella prenoidosa[C].Summary of the Fifth National Conference on Environmental Chemistry,2009(in Chinese)
• [45]Sun H,Zhang X,Zhang Z,et al.Influence of titanium dioxide nanoparticles on speciation and bioavailability of arsenite[J].Environmental Pol ution,2009,157(4):1165-1170
• [46]Tian S,Zhang Y,Song C,et al.Bioaccumulation and biotransformation of polybrominated diphenyl ethers in the marine bivalve(Scapharca subcrenata):Influence of titanium dioxide nanoparticles[J].Marine Pollution Bul etin,2015,90(1):48-53
• [47]Tian S,Zhang Y,Song C,et al.Titanium dioxide nanoparticles as carrier facilitate bioaccumulation of phenanthrene in marine bivalve,ark shell(Scapharca subcrenata)[J].Environmental Pollution,2014,192:59-64
• [48]Dalai S,Pakrashi S,Bhuvaneshwari M,et al.Toxic effect of Cr(VI)in presence of n-Ti O2and n-Al2O3particles towards freshwater microalgae[J].Aquatic Toxicology,2014,146:28-37
• [49]Tang Y,Li S,Qiao J,et al.Synergistic effects of nanosized titanium dioxide and zinc on the photosynthetic capacity and survival of Anabaena sp[J].International Journal of Molecular Sciences,2013,14(7):14395-14407
• [50]Porter A E,Gass M,Mul er K,et al.Direct imaging of single-walled carbon nanotubes in cells[J].Nature Nanotechnology,2007,2(11):713-717
• [51]Maysinger D,Lovric J,Eisenberg A,et al.Fate of micelles and quantum dots in cells[J].European Journal of Pharmaceutics and Biopharmaceutics,2007,65(3):270-281
• [52]Xia T,Kovochich M,Liong M,et al.Cationic polystyrene nanosphere toxicity depends on cell-specific endocytic and mitochondrial injury pathways[J].ACS Nano,2008,2(1):85-96
• [53]Orr G,Panther D J,Phillips J L,et al.Submicrometer and nanoscale inorganic particles exploit the actin machinery to be propelled along microvilli-like structures into alveolar cells[J].ACS Nano,2007,1(5):463-475
• [54]Zhang W,Xiong B,Sun W F,et al.Acute and chronic toxic effects of bisphenol A on Chlorella pyrenoidosa and Scenedesmus obliquus[J].Environmental Toxicology,2014,29(6):714-722
• [55]梁永莉,季宏兵,朱毅.莱茵衣藻的双酚A毒性效应及生物富集和降解作用研究[J].环境科学与技术,2014,6:25-36,96Liang Y,Ji H,Zhu Y.Research of bioauumulation and biodegradation of bisphenol A by Chlamydomonas reinhardtii[J].Environmental Science and Technology,2014,6:25-31,96(in Chinese)
• [56]王震宇,赵建,李娜,等.人工纳米颗粒对水生生物的毒性效应及其机制研究进展[J].环境科学,2010(6):1409-1418Wang Z Y,Zhao J,Li N,et al.Review of ecotoxicity and mechnism of engineered nanoparticles to aquatic organisms[J].Environmental Science,2010(6):1409-1418(in Chinese)