谷盼妮;王美娥;陈卫平;

• 1:中国科学院生态环境研究中心城市与区域生态国家重点实验室
• 2:中国科学院大学

摘要(Abstract)：

环草隆是北方城市应用较为广泛的一种草坪除草剂,重金属镉是土壤中常见的重要污染物,二者在土壤生态系统中存在联合暴露的潜在风险。为了准确评价城市绿地土壤重金属和农药复合污染的生态风险,选择不同重金属污染程度的土壤为研究对象,在室内模拟条件下,探讨了环草隆与镉复合污染对土壤有机氮矿化量、基础呼吸及土壤酶活性的影响。研究发现:环草隆与镉复合污染对各试验指标的影响均达到极显著水平(P<0.01);与镉复合污染能明显改变环草隆的微生物毒性效应,并且Cd对环草隆毒性效应的影响与二者浓度配比有很大关系,Cd浓度由1 mg·kg-1升高到10 mg·kg-1,环草隆浓度与样点G土壤有机氮矿化量变化率的线性关系由正相关变为负相关;复合污染下,根据剂量-效应关系计算的EC50值27.6~848mg·kg-1,远远高于环草隆草坪建议施用量(3.33 mg·kg-1);土壤中环草隆与镉复合污染的联合毒性效应受土壤理化性质及重金属含量影响较大,另外,二者复合效应也会随污染物浓度及试验指标的不同而不同。以上研究结果能够为城市土壤重金属和除草剂复合污染生态风险评价提供基础数据和技术方法。

关键词(KeyWords)： 环草隆;镉;城市土壤;氮矿化;基础呼吸;土壤酶;剂量-效应关系

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(41271503)

作者(Author): 谷盼妮;王美娥;陈卫平;

Email:

DOI:

参考文献(References)：

• [1]张甘霖,赵玉国,杨金玲,等.城市土壤环境问题及其研究进展[J].土壤学报,2007,44(5):925-933Zhang G L,Zhao Y G,Yang J L,et al.Urban soil environment issues and research progresses[J].Acta Pedologica Sinica,2007,44(5):925-933(in Chinese)
• [2]Lu Y,Gong Z T,Zhang G L,et al.Concentrations and chemical speciations of Cu,Zn,Pb and Cr of urban soils in Nanjing,China[J].Geoderma,2003,115(1-2):101-111
• [3]高飞,车少臣.城市绿地化学农药面源污染控制与无公害城市绿地建设[J].北京园林,2009,25(89):41-43
• [4]裴绍峰,刘海月,叶思源.我国南方主要城市土壤有机氯农药残留及分布特征[J].山东农业大学学报:自然科学版,2014,45(5):768-774Pei S F,Liu H Y,Ye S Y.Residues and distribution character of organochlorine pesticides in soil of main southern cities of China[J].Journal of Shandong Agricultural University:Natural Science Edition,2014,45(5):768-774(in Chinese)
• [5]Chen T B,Zheng Y M,Lei M,et al.Assessment of heavy metal pollution in surface soils of urban parks in Beijing,China[J].Chemosphere,2005,60(4):542-551
• [6]任向辉,王运兵.黑麦草草坪杂草防除的药剂实验[J].山西农业科学,2008,36(12):72-73Ren X H,Wang Y B.Drug-efficiency experiment on weeds control of ryegrass lawn[J].Journal of Shanxi Agricultural Sciences,2008,36(12):72-73(in Chinese)
• [7]Willis J B,Beam J B,Barker W L,et al.Weed control options in spring-seeded tall fescue(Festuca arundinacea)[J].Weed Technology,2006,20(4):1040-1046
• [8]Mc Elroy J S,Breeden G K.Tall fescue seedling tolerance to carfentrazone,bromoxynil,quinclorac,and siduron[J].Hort Science,2006,41(1):252-254
• [9]Kong L X,Kadokami K,Wang S P,et al.Monitoring of 1300 organic micro-pollutants in surface waters from Tianjin,North China[J].Chemosphere,2015,122:125-130
• [10]Fields M L,Hemphill D D.Influence of siduron and its degradation products on soil microflora[J].Weed Science,1968,16(4):417-419
• [11]Belasco I J,Langsdorf W P.Synthesis of C14-labeled siduron and its fate in soil[J].Journal of Agricultural and Food Chemistry,1969,17(5):1004-1007
• [12]熊严军.我国土壤污染现状及治理措施[J].现代农业科技,2010(8):294-296
• [13]Wang J H,Lu Y T,Shen G Q.Combined effects of cadmium and butachlor on soil enzyme activities and microbial community structure[J].Environmental Geology,2007,51(7):1221-1228
• [14]Wang J H,Ding H,Lu Y T,et al.Combined effects of cadmium and butachlor on microbial activities and community DNA in a paddy soil[J].Pedosphere,2009,19(5):623-630
• [15]律泽,胡筱敏,安婧,等.佳乐麝香和镉复合污染对土壤中放线菌数量的影响[J].生态学杂志,2014,33(6):1501-1507Lv Z,Hu X M,An J,et al.Joint effects of galaxolide and cadmium on actinomycetes quantities in soils[J].Chinese Journal of Ecology,2014,33(6):1501-1507(in Chinese)
• [16]Zhao Y P,Tan Y Y,Guo Y,et al.Interactions of tetracycline with Cd(Ⅱ),Cu(Ⅱ)and Pb(Ⅱ)and their cosorption behavior in soils[J].Environmental Pollution,2013,180:206-213
• [17]Perrodin Y,Boillot C,Angerville R,et al.Ecological risk assessment of urban and industrial systems:A review[J].Science of the Total Environment,2011,409(24):5162-5176
• [18]赵祥伟,骆永明,滕应,等.重金属复合污染农田土壤的微生物群落遗传多样性研究[J].环境科学学报,2005,25(2):186-191Zhao X W,Luo Y M,Teng Y,et al.Genetic diversity ofmicrobial communities in farmland soils contaminated with mixed heavy metals[J].Acta Scientiae Circumstantiae,2005,25(2):186-191(in Chinese)
• [19]滕应,骆永明,李振高.土壤重金属复合污染对脲酶、磷酸酶及脱氢酶的影响[J].中国环境科学,2008,28(2):147-152Teng Y,Luo Y M,Li Z G.Kinetics characters of soil urease,acid phosphotase and dehydrogenase activities in soil contaminated with mixed heavy metals[J].China Environmental Science,2008,28(2):147-152(in Chinese)
• [20]曹靖,贾红磊,徐海燕,等.干旱区污灌农田土壤Cu、Ni、复合污染与土壤酶活性的关系[J].农业环境科学学报,2008,27(5):1809-1814Cao J,Jia H L,Xu H Y,et al.Relationships between soil enzymatic activities and Cu-Ni compounds pollution in the waste water irrigated farmland in arid region[J].Journal of Agro-Environment Science,2008,27(5):1809-1814(in Chinese)
• [21]周启星.复合污染生态学[M].北京:中国环境科学出版社,1995:35-78
• [22]张慧,党志,姚丽贤,等.镉芘单一污染和复合污染对土壤微生物生态效应的影响[J].农业环境科学学报,2007,26(6):2225-2230Zhang H,Dang Z,Yao L X,et al.Eco-toxicological effect of cadmium and pyrene combined and simplex pollution on soil microbe[J].Journal of Agro-Environment Science,2007,26(6):2225-2230(in Chinese)
• [23]傅丽君,刘智任,杨磊.镉、铜、铅复合污染对枇杷园土壤微生物的生态毒理效应[J].生态毒理学报,2009,4(6):881-888Fu L J,Liu Z R,Yang L.Ecotoxicological effect of CdCu-Pb compound pollution on loquat soil microbe[J].Asian Journal of Ecotoxicology,2009,4(6):881-888(in Chinese)
• [24]Wang M E,Zhou Q X.Joint stress of chlorimuron-ethyl and cadmium on wheat Triticum aestivum at biochemical levels[J].Environmental Pollution,2006,144(2):572-580
• [25]李章平,陈玉成,杨学春,等.重庆市主城区土壤重金属的潜在生态危害评价[J].西南农业大学学报:自然科学版,2006,28(2):227-230Li Z P,Chen Y C,Yang X C,et al.Assessment of potential ecological hazard of heavy metals in urban soils in Chongqing city[J].Journal of Southwest Agricultural University:Natural Science,2006,28(2):227-230(in Chinese)
• [26]Nam J J,Thomas G O,Jaward F M,et al.PAHs in background soils from Western Europe:Influence of atmospheric deposition and soil organic matter[J].Chemosphere,2008,70(9):1596-1602
• [27]Gosewinkel U,Broadbent F.Conductimetric determination of soil urease activity[J].Communications in Soil Science&Plant Analysis,1984,15(11):1377-1389
• [28]Tabatabai M,Bremner J.Arylsulfatase activity of soils[J].Soil Science Society of America Journal,1970,34(2):225-229
• [29]Frankeberger W,Johanson J.Method of measuring invertase activity in soils[J].Plant and Soil,1983,74(3):301-311
• [30]Kramer M,Yerdei G.Application of the method of phosphatase activity determination in agricultural chemistry[J].Soviet Soil Science,1959,9:1100-1103
• [31]鲍士旦.土壤农化分析(第三版)[M].北京:中国农业出版社:2008,58-59
• [32]许光辉,郑元洪.土壤微生物分析方法手册[M].北京:中国农业出版社,1986:226-227,274-279
• [33]周启星,孔繁翔,朱琳.生态毒理学[M].北京:科学出版社,2004:178-280
• [34]Chen Q L,Yang B S,Wang H,et al.Soil microbial community toxic response to atrazine and its residues under atrazine and lead contamination[J].Environmental Science and Pollution Research,2015,22(2):996-1007
• [35]胡著邦,汪海珍,吴建军,等.镉与苄嘧磺隆除草剂单一污染和复合污染土壤的微生物生态效应[J].浙江大学学报:农业与生命科学版,2005,31(2):151-156Hu B Z,Wang H Z,Wu J J,et al.Ecological effects of both single and combined pollution of Cd and bensulfuron-methyl on soil microorganisms[J].Journal of Zhejiang University:Agriculture&Life Sciences,2005,31(2):151-156(in Chinese)
• [36]陈莹莹,王金花,陆贻通.丁草胺与镉复合污染对土壤呼吸强度的影响[J].环境污染与防治,2006,28(10):723-730Chen Y Y,Wang J H,Lu Y T.Effects of butachlor and Cd on microbial respiration in top soil[J].Environmental Pollution&Control,2006,28(10):723-730(in Chinese)
• [37]沈国清,陆贻通,洪静波.重金属和多环芳烃复合污染对土壤酶活性的影响及定量表征[J].应用与环境生物学报,2005,11(4):479-482Shen G Q,Lu Y T,Hong J B.Quantitative expression and effect of combined pollution of heavy metals and polycyclic acromatic hydrocarbons on soil enzyme[J].Chinese Journal of Applied and Environmental Biology,2005,11(4):479-482(in Chinese)
• [38]Chen C H,Zhou Q X,Cai Z,et al.Effects of soil polycyclic musk and cadmium on pollutant uptake and bio-chemical responses of wheat(Triticum aestivum)[J].Archives of Environmental Contamination and Toxicology,2010,59(4):564-573
• [39]王果.Cu2+对3种农药在Ca-蒙脱石和δ-Al2O3上吸附的影响[J].环境科学学报,1996,16(1):23-29Wang G.Adsorption of three pesticides on Ca-montmorillonite andδ-Al2O3in presence or absence of copper[J].Acta Scientiae Circumstantiae,1996,16(1):23-29(in Chinese)
• [40]Moreau C J,Klerks P L,Haas C N.Interaction between phenanthrene and zinc in their toxicity to the sheepshead minnow(Cyprinodon variegates)[J].Archives of Environmental Contamination and Toxicology,1999,37(2):251-257
• [41]Teisseire H,Couderchet M,Vernet G.Toxic responses and catalase activity of Lemna minor L.exposed to folpet,copper,and their combination[J].Ecotoxicology and Environment Safety,1998,40(3):194-200
• [42]黄欣,陆贻通.镉对土壤丁草胺降解作用的影响[J].环境污染与防治,1991,13(1):13-15Hang X,Lu Y T.The effects of Cd on the degradation of butachlor in soil[J].Environmental Pollution&Control,1991,13(1):13-15(in Chinese)