李然;徐应明;王林;谷朋磊;孙约兵;梁学峰;

• 1:沈阳农业大学土地与环境学院
• 2:农业部环境保护科研监测所
• 3:农业部产地环境污染防控重点实验室

摘要(Abstract)：

采用水培试验,研究Cd胁迫(10μmol·L~(-1))条件下,不同浓度Mn(0,0.1,1,10,100μmol·L~(-1))处理对2种镉累积能力不同的油菜生物量、Cd和Mn含量以及根系形态参数的影响。结果表明,与正常营养处理Mn1相比,缺Mn和Mn过量处理显著降低2种油菜的地上部和镉低积累油菜"华骏"的根部生物量,而对普通油菜"寒绿"的根部生物量没有显著影响。与Mn1处理相比,缺Mn处理下2种油菜的Cd累积总量和"华骏"地上部Cd含量显著降低;Mn过量处理下2种油菜根部和地上部Cd含量均呈升高趋势,2种油菜的Cd累积总量无显著变化或显著降低。随Mn处理浓度升高,2种油菜地上部和根部Mn含量显著升高,缺Mn处理下2种油菜根部的Mn含量低于Mn缺乏的临界值。与Mn1处理相比,缺Mn处理下"华骏"的根长、表面积、体积、平均直径和根尖数显著降低;Mn过量处理下"寒绿"的根长以及"华骏"的根系表面积、体积显著降低;缺Mn处理下2种油菜细根表面积所占的比例增加,粗根表面积比例降低;Mn过量时,"寒绿"的粗根表面积比例增加,细根比例降低,而"华骏"则相反。总的来看,缺Mn处理影响油菜根系和地上部生长,减少地上部Cd含量和植株Cd累积总量;Mn过量处理下油菜生长也受到抑制,但是油菜地上部和根部Cd含量有所升高;2种油菜的根系形态学参数在不同Mn处理下变化规律不同,表现出不同的响应策略。

关键词(KeyWords)： 镉;锰;油菜;交互作用;低积累品种;根系形态

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(41571322);; 天津市科技支撑计划重点项目(14ZCZDSF00004);; 天津市农业科技成果转化与推广项目(201404100)

作者(Author): 李然;徐应明;王林;谷朋磊;孙约兵;梁学峰;

Email:

DOI:

参考文献(References)：

• [1]环境保护部,国土资源部.全国土壤污染状况调查公报[EB/OL].(2014-04-17)[2017-07-19].http://www.mlr.gov.cn/xwdt/jrxw/201404/t20140417_1312998.htm.
• [2]Zeng X B,Li L F,Mei X R,et al.Heavy metal content in Chinese vegetable plantation land soils and related source analysis[J].Agricultural Sciences in China,2008,7(9):1115-1126
• [3]徐震,田丽梅,江应松,等.天津市污灌区农田环境质量现状分析[J].天津农林科技,1999,152(6):26-28Xu Z,Tian L M,Jiang Y S,et al.Analysis on present situation of farmland environmental quality in sewage irrigation district of Tianjin[J].Tianjin Agricultural Science and Tecnology,1999,152(6):26-28(in Chinese)
• [4]师荣光,赵玉杰,高怀友,等.天津市郊蔬菜重金属污染评价与特征分析[J].农业环境科学学报,2005,24(S1):169-173Shi R G,Zhao Y J,Gao H Y,et al.Metals influencing the level of heavy metal contamination of vegetables in Tianjin City as derived by principal components anlysis[J].Journal of Agro-Environment Science,2005,24(S1):169-173(in Chinese)
• [5]Videa J P,Lopez M L,Narayan M,et al.The biochemistry of environmental heavy metal uptake by plants:Implications for the food chain[J].The International Journal of Biochemistry and Cell Biology,2009,41(8-9):1665-1677
• [6]Sasaki A,Yamaji N,Yokosho K,et al.Nramp5 is a major transporter responsible for manganese and cadmium uptake in rice[J].The Plant Cell,2012,24(5):2155-2167
• [7]Yang M,Zhang Y Y,Zhang L J,et al.Nramp5 contributes to manganese translocation and distribution in rice shoots[J].Journal of Experimental Botany,2014,65(17):4849-4861
• [8]蓝兰,喻华,冯文强,等.不同中微量及有益元素对小麦吸收镉的影响[J].水土保持学报,2010,24(5):54-58Lan L,Yu H,Feng W Q,et al.Effects of secondary,micro-and benificial elements on wheat growth and cadmium uptake[J].Journal of Soil and Water Conservation,2010,24(5):54-58(in Chinese)
• [9]覃都.水稻镉积累的锰、氮调控研究[D].重庆:西南大学,2009:63Tan D.Investigation of manganese,nitrogen regulation on cadmium accumulation in rice plants[D].Chongqing:Southwest University,2009:63(in Chinese)
• [10]周相玉,冯文强,秦鱼生,等.镁、锰、活性炭和石灰及其交互作用对小麦镉吸收的影响[J].生态学报,2013,33(14):4289-4296Zhou X Y,Feng W Q,Qin Y S,et al.Effects of magnesium,manganese,activated carbon and lime and their interactions on cadmium uptaked by wheat[J].Acta Ecologica Sinica,2013,33(14):4289-4296(in Chinese)
• [11]Hernández L E,Lorodrgue E L,Gárate A,et al.Influence of cadmium on the uptake,tissue accumulation and subcellular distribution of manganese in pea seedlings[J].Plant Science,1998,132(2):139-151
• [12]Peng K J,Luo C L,You W X,et al.Manganese uptake and interactions with cadmium in the hyperaccumulator—Phytolacca americana L[J].Journal of Hazardous Materials,2008,154(1-3):674-681
• [13]Liu H M,Zhang Y X,Chai T Y,et al.Manganese-mitigation of cadmium toxicity to seedling growth of Phytolacca acinosa Roxb.is controlled by the manganese/cadmium molar ratio under hydroponic conditions[J].Plant Physiology and Biochemistry,2013,73:144-153
• [14]Zornoza P,Sánchezpard O B,Carpena R O.Interaction and accumulation of manganese and cadmium in the manganese accumulator Lupinus albus[J].Journal of Plant Physiology,2010,167:1027-1032
• [15]何俊瑜,王阳阳,任艳芳,等.镉胁迫对不同水稻品种幼苗根系形态和生理特性的影响[J].生态环境学报,2009,18(5):1863-1868He J Y,Wang Y Y,Ren Y F,et al.Effect of cadimium on root morphology and physiology characteristics of rice seedlings[J].Ecology and Environment,2009,18(5):1863-1868(in Chinese)
• [16]Wang L,Xu Y M,Sun Y B,et al.Identification of pakchoi cultivars with low cadmium accumulation and soil factors that affect their cadmium uptake and translocation[J].Frontiers of Environmental Science&Engineering,2014,8(6):877-887
• [17]王景安,张福锁.锌对不同基因型玉米缺锌后的恢复效果及胚乳在缺锌中的作用[J].中国生态农业学报,2003,11(3):78-81Wang J A,Zhang F S.Effects of different zinc concentrations on maize restoration after zinc deficiency and the function of endosperm in zinc deficency[J].Chinese Journal of Eco-Agriculture,2003,11(3):78-81(in Chinese)
• [18]代晶晶,徐应明,王林,等.不同锌营养下喷施锌肥对油菜生长和元素含量的影响[J].环境化学,2017,36(5):1017-1025Dai J J,Xu Y M,Wang L,et al.Effect of foliar zinc application on growth and elements concentrations of pak-choi under different zinc nutrition status[J].Environmental Chemistry,2017,36(5):1017-1025(in Chinese)
• [19]张玉秀,李林峰,柴团耀,等.锰对植物毒害及植物耐锰机理研究进展[J].植物学报,2010,45(4):506-520Zhang Y X,Li L F,Chai T Y,et al.Mechanisms of manganses toxicity and manganese tolerance in plants[J].Chinese Bulletin of Botany,2010,45(4):506-520(in Chinese)
• [20]Xin J L,Huang B F,Dai H W,et al.Characterization of cadmium uptake,translocation,and distribution in young seedlings of two hot pepper cultivars that differ in fruit cadmium concentration[J].Environmental Science and Pollution Research,2014,21(12):7449-7456
• [21]安振锋,方正.植物锰营养研究进展[J].河北农业科学,2002,4(6):35-42An Z F,Fang Z.The advance of manganese nutrition in plant[J].Journal of Hebei Agricultural Sciences,2002,4(6):35-42(in Chinese)
• [22]陆景陵.植物营养学(上册)[M].北京:中国农业大学出版社,2005:87-91Lu J L.Plant Nutrition(volime one)[M].Beijing:China Agricultural University Press,2005:87-91(in Chinese)
• [23]张福锁,吕世华,方正.不同小麦品种(品系)耐缺锰能力的比较研究[J].植物营养与肥料学报,1998,4(3):277-283Zang F S,Lv S H,Fang Z.Sdudy on tolerance of different wheat cultivars or lines to manganese deficiency[J].Journal of Plant Nutrition and Fertilizer,1998,4(3):277-283(in Chinese)
• [24]严炜.大豆适应锰毒害的生理和分子机理研究[D].海口:海南大学,2010:58Yan W.Physiological and molecular mechanisms of soybean(Glycine max)adaptation to manganese stress[D].Haikou:Hainan University,2010:58(in Chinese)
• [25]任立民,刘鹏,蔡妙珍,等.水蓼、小飞蓬、杠板归和美洲商陆对锰毒的生理响应[J].水土保持学报,2007,21(3):81-85Ren L M,Liu P,Cai M Z,et al.Physiological response of Polygonum hydropiper,Comnyza canadensis,Polygonum perfoliatum and Phytolacca americana to manganese toxicity[J].Jounral of Soil and Water Conservation,2007,21(3):81-85(in Chinese)
• [26]Wang R G,Dai S X,Tang S R,et al.Growth,gas exvhange,root morphology and cadmium uptake responses of poplars and willows grown on cadmium-contaminated soil to elevated CO2[J].Environmental Earth Sciences,2012,67(1):1-13
• [27]屈佳伟,高聚林,王志刚,等.玉米根系特征的基因型差异及与氮吸收效率的关系[J].玉米科学,2016,24(2):72-78Qu J W,Gao J L,Wang Z G,et al.Genotype differences in root characteristics by maize and its relations to nitrogen uptake efficiency[J].Journal of Maize Science,2016,24(2):72-78(in Chinese)
• [28]Ding Y Z,Feng R W,Wang R G,et al.A dual effect of Se on Cd toxicity:Evidence from plants growth,root morphology and responses of the antioxidative systems of paddy rice[J].Plant and Soil,2014,375(1-2):289-301
• [29]Jia Y,Tang S R,Ju X H,et al.Effects of elevated CO2levels on root morphological traits and Cd uptakes of two lolium species under Cd stress[J].Journal of Zhejiang University-Science,2011,12(4):313-325