郭敏;龚继来;曾光明;

• 1:湖南大学环境科学与工程学院
• 2:湖南大学环境生物与控制教育部重点实验室

摘要(Abstract)：

考察了浓度为10、50和100 mg·L~(-1)的多壁碳纳米管(MWCNTs)的植物毒性,通过测量水稻生长指数、根系氧化应激反应、细胞膜损伤和生理功能的变化,探索了其具体的毒性机制。将水稻幼苗暴露于不同浓度的MWCNTs悬浮液中培养10 d后,高浓度处理组(100 mg·L~(-1))中水稻幼苗地上部分和根系鲜重分别降低至对照组的87.6%±1.1%和69.2%±7.8%。对根系深入研究发现氧化应激反应和细胞膜的损伤主要出现在高浓度处理组,此时过氧化氢酶(CAT)活性由对照组的(8.8±1.6)U·mg-1prot(protein,蛋白质)增加至(16.3±2.8)U·mg-1prot,丙二醛(MDA)含量由对照组的(8.0±0.3)μmol·g-1FW(fresh weight,鲜重)增加至(15.1±1.4)μmol·g-1FW。然而,水稻根系的生化酶活性在低浓度(10 mg·L~(-1))时就开始明显降低。通过透射电镜(TEM)观察发现,MWCNTs颗粒分布在水稻幼苗根系细胞内,从而证实了MWCNTs能被植物细胞吸收。

关键词(KeyWords)： 多壁碳纳米管(MWCNTs);水稻幼苗;植物毒性;根系损伤;氧化应激;细胞膜损伤

Abstract:

Keywords:

基金项目(Foundation): 湖南大学交叉学科项目(531107040761);; 国家自然科学基金(51579095)

作者(Author): 郭敏;龚继来;曾光明;

Email:

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