北方某市城区水环境中轮状病毒的初步检测Detection of Rotaviruses in Urban Water Samples from a City in North China
程莉;何晓青;李伟;马梅;王子健;谢响明;
摘要(Abstract):
轮状病毒(Rotavirus,RV)是引起全世界婴幼儿肠胃炎的最主要病原体.在我国,儿童秋季腹泻发病达到高峰,也主要由轮状病毒引起.论文改进了RT-nested-PCR检测水中轮状病毒的方法,于2006年10月对北方某市城区水环境(包括污水、地表水和饮用水)中的轮状病毒进行了初步检测.结果表明,该市某污水厂进水和不同工艺段出水、主要公园的景观水体、主要自来水厂进水和出水以及城区管网水,均检出轮状病毒.综合分析从水源到管网的过程,认为管网水中的轮状病毒可能与水源水污染有关.管网水中有轮状病毒检出,说明秋季该市饮用水中存在潜在的轮状病毒传染源.同期对管网水进行的细菌学指标检测,没有发现总大肠菌群、耐热大肠菌群、粪链球菌,可见目前城市供水水质标准中的细菌学指标并不能准确反映病毒污染状况.
关键词(KeyWords): 轮状病毒;污水;地表水;饮用水;检测
基金项目(Foundation): 国家重点基础研究发展规划(973)项目(No.2007CB407304);; 北京市自然科学基金项目(No.8061004);; 北京林业大学学生自选课题基金项目(No.06jj052)
作者(Author): 程莉;何晓青;李伟;马梅;王子健;谢响明;
Email:
DOI:
参考文献(References):
- Abbaszadegan M,Stewart P,LeChevallier M.1999.A strategy fordetection of viruses in groundwater by PCR[J].Appl EnvironMicrobiol,2:444-449
- Baggi F,Demarta A,Peduzzi R.2001.Persistence of viral pathogens and bacteriophages during sewage treatment:lack of correlationwith indicator bacteria[J].Res Microbiol,152:743-751
- Baggi F,Peduzzi R.2000.Genotyping of rotaviruses in environmentalwater and stool samples in southern Switzerland by nucleotidesequence analysis of189base pairs at the5’end of the VP7gene[J].J Clin Microbiol,38(10):3681-3685
- Boom R,Sol C J,Salimans M M,Jansen C L,Wertheim-van Dillen PM,Van der Noordaa J.1990.Rapid and simple method forpurification of nucleic acids[J].J Clin Microbiol,28:495-503
- Borchardt M A,Haas N L,Hunt R J.2004.Vulnerability ofdrinking-water wells in La Crosse,Wisconsin,to enteric-viruscontamination from surface water contributions[J].Appl EnvironMicrobiol,70(10):5937-5946
- Deetz T R,Smith E M,Goyal S M,Gerba C P,Vollet J J,Tsai L,Dupont H L,Keswick B H.1984.Occurrence ofrotavirus and enterovirus indrinking and environmental waters in adeveloping nation[J].Water Res,18:567-572
- Fang Z Y,Ye Q,Ho M S,Dong H,Qing S,Penaranda M E,HungT,Wen L,Glass R I.1989.Investigation of an outbreak of adultdiarrhea rotavirus in China[J].J Infect Dis,160:948-953
- Fout G S,Martinson B C,Moyer M W,Dahling D R.2003.Amultiplex reverse transcription-PCR method for detection of humanenteric viruses in groundwater[J].Appl Environ Microbiol,69:3158-3164
- Gallay A,De Valk H,Cournot M,Ladeuil B,Hemery C,CastorC,Bon F,Mégraud F,Le Cann P,Desenclos J C.2006.Alarge multi-pathogen waterborne community outbreak linked to faecalcontamination of a groundwater system,France,2000[J].ClinMicrobiol Infect,12:561-570
- Glass R I,Fang Z Y,Jiang B M.2005.Introduction[C].Pcoceedingof the2nd international workshop on rotavirus vaccine.Beijing
- Gratacap-cavallier B,Genoulaz O,Brengel-Pesce K,Soule H,Innocenti-Francillard P,Bost M,Gofti L,Zmirou D,Seigneurin JM.2000.Detection of human and animal rotavirus sequences indrinking water[J].Appl Environ Microbiol,66:2690-2692
- Griffin D W,Gibson III C J,Lipp E K,Riley K,Paul III J H,Rose J B.1999.Detection of viral pathogens by reversetranscriptase PCR and of microbial indicators by standard methodsin the canals of the Florida Keys[J].Appl Environ Microbiol,65:4118-4125
- Hafliger D,Hubner P,Luthy J.2000.Outbreak of viralgastroenteritis due to sewage-contaminated drinking water[J].IntJ Food Microbiol,54:123-126
- Hung T,Chen G,Wang C,Yao H,Fang Z,Chao T,Chou Z,Ye W,Chang X,Den S,Liong X,Chang W.1984.Waterborneoutbreak of rotavirus diarrhoea in adults in China caused by anovel rotavirus[J].Lancet,1(8387):1139-1142
- Jean J,Blais B,Darveau A,Fliss I.2002.Rapid detection ofhuman rotavirus using colorimetric nucleic acid sequence-basedimmunosorbent assay in amplification(NASBA)-enzyme-linked sewagetreatment effluent[J].FEMS Microbiol Lett,210:143-147
- Jiang S C,Chu W.2004.PCR detection of pathogenic viruses in southern California urban rivers[J].J Appl Microbiol,97:17-28
- Keswick B H,Gerba C P,DuPont H L,Rose J B.1984.Detection of enteric viruses in treated drinking water[J].ApplEnviron Microbiol,47:1290-1294
- Lodder W J,De Roda Husman A M.2005.Presence ofnoroviruses and other enteric viruses in sewage and surface watersin the Netherlands[J].Appl Environ Microbiol,3:1453-1461
- Myrmel M,Berg E M,Grinde B,Rimstad E.2006.Entericviruses in inlet and outlet samples from sewage treatment plants[J].J Water Health,4(2):197-209
- Parashar U D,Hummelman E G,Bresee J S,Miller M A,GlassR I.2003.Global illness and deaths caused by rotavirus diseasein children[J].Emerg Infect Dis,9:565-572
- Pusch D,Oh D Y,Wolf S,Dumke R,Schroter-Bobsin U,HohneM,Roske I,Schreier E.2005.Detection of enteric viruses andbacterial indicators in German environmental waters[J].Arch Virol,150:929-947
- Qian Y.2005.Virology of rotavirus and diagnosis in laboratory[C].The Proceeding of the Second International Workshop onRotavirus Vaccine.Beijing(in Chinese)
- Rzezutka A,Cook N.2004.Survival of human enteric viruses in theenvironment and food[J].FEMS Microbiology Reviews,28:441-453
- Soule H,Genoulaz O,Gratacap-Cavallier B,Chevallier P,Liu JX,Seigneurin J M.2000.Ultrafiltration and reverse transcription-polymerase chain reaction:an efficient process for poliovirus,rotavirus and hepatitis A virus detection in water[J].Water Res,34:1063-1067
- Ministry of Health of P.R.China.2001.GB5750-85Standardexamination methods for drinking water[S].Beijing(in Chinese)
- Tsai Y L,Tran B,Sangermano L R,Palmer C J.1994.Detection of poliovirus,hepatitis A virus,and rotavirus fromsewage and ocean water by triplex reverse transcriptase PCR[J].Appl Environ Microbiol,60:2400-2407
- Wang B,Fang Z Y,Gao Q.2005.The economic burden of diarreacaused by rotavirus in China[C].The Proceeding of the SecondInternational Workshop on Rotavirus Vaccine.Beijing(in Chinese)
- Wyn-Jones A P,Sellwood J.2001.Enteric viruses in the aquaticenvironment[J].J Appl Microbiol,91:945-962
- Zhang Z Z,Zhang Z,Liu Y X.1998.The study of a infectoncaused by novel adult rotaviruses in one university[J].DiseaseSurveillance,13:5-6(in Chinese)
- 钱渊.2005.轮状病毒的病毒学及实验室诊断[C].第二届国际轮状病毒疫苗研讨会.北京
- 中华人民共和国卫生部.2001.GB5750-85生活饮用水检验规范[S].北京
- 王蓓,方肇寅,高倩.2005.中国轮状病毒腹泻的经济负担浅析[C].第二届国际轮状病毒疫苗研讨会.北京
- 张志珍,张忠,刘玉欣.1998.某大学一起新型成人轮状病毒感染调查[J].疾病监测,13:5-6