铀胁迫对两种蓝藻生长及抗氧化酶活性的影响Effects of Uranium Stress on Growth and Activity of Antioxidant Enzymes of Two Cyanobacteria
唐东山;张容芳;周孙林;吕俊文;张晓文;谢水波;
摘要(Abstract):
研究了不同浓度铀(0.1、1.0、10.0和30.0mg·L-1)胁迫对满江红鱼腥藻(Anabaena azollae)和爪哇伪枝藻(Scytonemaj avanicum)生长及抗氧化酶活性的影响。结果表明,低浓度铀(0.1和1.0mg·L-1)条件下,两种蓝藻的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性及丙二醛(MDA)含量均无明显变化;叶绿素a含量和可溶性蛋白质含量均较对照上升,表明低浓度铀对A.azollae和S.javanicum均未形成明显胁迫,对生长表现为刺激和促进作用。10.0mg·L-1浓度的铀对A. azollae的生长影响不大,对S. javanicum却产生了明显的抑制作用。30.0mg·L-1铀胁迫下,两种蓝藻中的MDA显著增加,抗氧化酶系统受到破坏,生长严重受阻。实验结果显示,A. azollae和S. javanicum的耐铀极限分别在10.0~30.0mg·L-1和1.0~10.0mg·L-1之间,A. azollae的抗铀性比S. javanicum强。
关键词(KeyWords): 满江红鱼腥藻;爪哇伪枝藻;抗氧化酶
基金项目(Foundation): 湖南省自然科学基金(09JJ6046);; 湖南省高校重点实验室开放基金(09K071);; 国家自然科学基金项目(11175081)联合资助
作者(Author): 唐东山;张容芳;周孙林;吕俊文;张晓文;谢水波;
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