刘云霞;周益奇;董妍;孙国新;

• 1:中国科学院生态环境研究中心

摘要(Abstract)：

采用接种和不接种菌根真菌(Glomus mosseae)两种模式,研究了菌根真菌对旱稻中砷积累的影响。结果表明接种菌根真菌能够明显提高旱稻地上部磷的含量(对照0.84g·kg-1,接种2.23g·kg-1)和地下部(对照0.76g·kg-1,接种1.04g·kg-1)对磷的吸收;降低地上部(对照2.40mg·kg-1,接种0.69mg·kg-1)和地下部(对照8.90mg·kg-1,接种4.87mg·kg-1)中砷的积累;提高磷从地下部向地上部的转运能力,从而有效抑制了砷从地下部到地上部的传输。进一步研究发现,菌根真菌还可以降低土壤溶液中AsIII和总砷含量,即菌根真菌能够降低水稻可获得的砷含量,从而减少砷对人体健康的威胁。

关键词(KeyWords)： 水稻;砷;菌根真菌

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(No.40973058);; 国家水体污染控制与治理科技重大专项(2009ZX07212-001-05)

作者(Author): 刘云霞;周益奇;董妍;孙国新;

Email:

DOI:

参考文献(References)：

• [1]Ontario Ministry of the Environment.Arsenic in the Environment[EB/OL].2001.http://www.ene.gov.on.ca/cons/3792e.htm
• [2]Meacher D M,Menzel D B,Dillencourt M D,et al.Estimation of multimedia inorganic arsenic intake in the US popula-tion[J].Human and Ecological Risk Assessment,2002,8(7):1697-1721
• [3]Meliker J R,Franzblau A,Slotnick M J,et al.Major contributors to inorganic arsenic intake in southeastern Michigan[J].International Journal of Hygiene and Environmental Health,2006,209(5):399411
• [4]Schoof R A,Yost L J,Crecelius E,et al.Dietary arsenic intake in Taiwanese districts with elevated arsenic in drinking water[J].Human and Ecological Risk Assessment,1998,4(1):117-135
• [5]Tsuji J S,Yost L J,Barraj L M,et al.Use of background inorganic arsenic exposures to provide perspective on risk as-sessment results[J].Regulatory Toxicology and Pharmacol-ogy,2007,48(1):5968
• [6]Williams P N,Raab A,Feldmann J,et al.Market basket survey shows elevated levels of As in south central U.S.pro-cessed rice compared to California:Consequences for human dietary exposure[J].Environmental Science&Technology,2007,41(7):21782183
• [7]Abedin M J,Cresser M S,Meharg A A,et al.Arsenic ac-cumulation and metabolism in rice(Oryza sativa L.)[J].Environmental Science&Technology,2002,36(5):962-968
• [8]陈怀满,郑春荣,涂从,等.中国土壤重金属污染现状与防治对策[J].人类环境杂志,1999,28(2):130-133Chen H M,Zheng C R,Xu C,et al.Metal pollution in soils in China:Status and countermeasures[J].AMBIO,1999,28(2):130133(in Chinese)
• [9]宋静,朱荫湄.土壤重金属污染修复技术[J].农业环境保护,1998,17(6):271-273
• [10]Khan A G,Kueck T M,Chaudrhry C S,et al.Role of plants,mycorrhizae and phytochelators in heavy metal con-taminated land remediation[J].Chemosphere,2000,41(12):197207
• [11]华建峰,林先贵,蒋倩.AM真菌对烟草砷吸收及根际pH的影响[J].生态环境学报,2009,18(5):1746-1752Hua J F,Lin X G,Jiang Q.Effects of arbuscular mycorrhizal inoculation on arsenic uptake and rhizosphere pH of Nicoti-ana tabacum L.[J].Ecology and Environmental Sciences,2009,18(5):17461752(in Chinese)
• [12]Leyval C,Turnau K,Haselwandter K.Effect of heavy metal pollution on mycorrhizal colonisation and function,physio-logical,ecological and applied aspects[J].Mycorrhiza,1997,7(3):139153
• [13]Zhu Y G,Laidlaw A S,Christie P,et al.The specificity of arbuscular mycorrhizal fungi in perennial ryegrass white clo-ver pasture,agriculture[J].Ecosystems and Environment,1999,77(3):211218
• [14]Chen B D,Tao H Q,Li X L,et al.The role of arbuscular mycorrhiza in zinc uptake by red clover growing in a calcare-ous soil spiked with various quantities of zinc[J].Chemo-sphere,2003,50(6):839846
• [15]Christie P,Li X L,Chen B D.Arbuscular mycorrhiza can depress translocation of zinc to shoots of host plants in soils moderately polluted with zinc[J].Plant and Soil,2004,261(1-2):209217
• [16]Galli U,Schuepp H,Brunold C.Heavy metal binding by mycorrhizal fungi[J].Physiologia Plantarum,1994,92(2):364-368
• [17]Janouskova M,Pavlikova D,Macek T,et al.Influence of arbuscular mycorrhiza on the growth and cadmium uptake of tobacco with inserted metallothionein gene[J].Applied Soil Ecology,2005,29(3):209214
• [18]Tullio M,Pierandrei F,Salemo A,et al.Tolerance to cad-mium of vesicular arbuscular mycorrhizae spores isolated from a cadmium-polluted and unpolluted soil[J].Biology and Fertility of Soils,2003,37(4):211214
• [19]夏运生,陈保冬,朱永官,等.外加不同铁源和丛枝菌根对砷污染土壤上玉米生长及磷、砷吸收的影响[J].环境科学学报,2008,28(3):516-524Xia Y S,Chen B D,Zhu Y G,et al.Effects of different iron sources and arbuscular mycorrhiza on the growth and phos-phorus and arsenic uptake by maize plants(Zea mays L.)in As contaminated soil[J].Acta Scientiae Circumstantiae,2008,28(3):516524(in Chinese)
• [20]白建峰,林先贵,尹睿,等.砷污染土壤的生物修复研究进展[J].土壤,2007,39(5):692-700Bai J F,Lin X G,Yin R,et al.Bio-remediation of arsenic-polluted soil:Recent advancements[J].Soils,2007,39(5):692-700
• [21]Dong Y,Zhu Y G,Smith F A.Arbuscular mycorrhiza en-hanced arsenic resistance of both white clover(Trifolium re-pens Linn.)and ryegrass(Lolium perenne L.)plants in an arsenic contaminated soil[J].Environmental Pollution,2008,155(1):174181
• [22]Pearson J N,Jackobsen I.The relative contribution of hyphae and roots to phosphorus uptake by arbuscular mycor-rhizal plants measured by dual labelling with32P and33P[J].New Phytologist,1993,124(3):489494
• [23]Sun G X,Williams P N,Zhu Y G,et al.Survey of arsenic and its speciation in rice products such as breakfast cereals,rice crackers and Japanese rice condiments[J].Environ-ment International,2009,35(3):473475
• [24]Sun G X,Williams P N,Carey A M,et al.Inorganic arsenic in rice bran and its products are an order of magnitude higher than in bulk grain[J].Environmental Science&Technology,2008,42(19):75427546
• [25]Giovannetti M,Mosse B.An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots[J].New Phytologist,1980,84(3):489-500
• [26]陈保冬.丛枝菌根减轻宿主植物锌、镉毒害机理研究[D].北京:中国农业大学,博士学位论文,2002
• [27]Ahmed F R S,Killham K,Alexander I.Influences of arbuscular mycorrhizal fungus Glomus mosseae on growth and nu-trition of lentil irrigated with arsenic contaminated water[J].Plant and Soil,2006,258(1-2):3341
• [28]Liu Y,Zhu Y G,Chen B D,et al.Yield and arsenate up-take of arbuscular mycorrhizal tomato colonized by Glomus mosseae BEG167in As spiked soil under glasshouse condi-tions[J].Environment International,2005,31(6):867-873
• [29]张美庆,王幼珊.铅铜镉砷对VA真菌侵染小麦根的影响[J].农业新技术,1990,3:47-48
• [30]Chen B D,Xiao X Y,Zhu Y G,et al.The arbuscular my-corrhizal fungus Glomus mosseae gives contradictory effects on phosphorus and arsenic acquisition by Medicago sativa Linn[J].Science of the Total Environment,2007,379(2-3):226234
• [31]Duan G L,Zhu Y G,Tong Y P,et al.Characterization of arsenate reductase in the extract of roots and fronds of Chi-nese brake fern,an arsenic hyperaccumulatior[J].Plant Physiology,2005,138(1):461469
• [32]Meharg A A,Macnair M R.Suppression of the high-affinity phosphate-uptake system:A mechanism of arsenate tolerance on Holcus Lanatus L.[J].Journal of Experimental Botany,1992,43(4):519524
• [33]Rosen B P.Biochemistry of arsenic detoxification[J].FEBS Letters,2002,529(1):8692
• [34]Chen B D,Zhu Y G,Duan J,et al.Effects of the arbuscular mycorrhizal fungus Glomus mosseae on growth and metal uptake by four plant species in copper mine tailings[J].Environmental Pollution,2007,147(2):374380
• [35]Sharples J M,Meharg A A,Chambers S M,et al.Mechanism of arsenate resistance in the ericoid mycorrhizal fungus Hymenoscyphus ericae[J].Plant Physiology,2000,124(3):1327-1334
• [36]Sharples J M,Meharg A A,Chambers S M,et al.Evolution:Symbiotic solution to arsenic contamination[J].Na-ture,2000,404:951952
• [37]Ye W L,Wood B A,Stroud J L,et al.Arsenic speciation in phloem and xylem exudates of castor bean[J].Plant Physi-ology,2010,154(3):15051513
• [38]孙国新.大米及其制品中砷含量及形态研究与健康风险评价[D].北京:中国科学院生态环境研究中心,博士后出站报告,2008